Results 71 to 80 of about 45,603 (283)
The Power of CRISPR-Cas9-Induced Genome Editing to Speed Up Plant Breeding
International Journal of Genomics, 2016 Genome editing with engineered nucleases enabling site-directed sequence modifications bears a great potential for advanced plant breeding and crop protection.
Hieu X. Cao +3 more
doaj +1 more source
CRF: detection of CRISPR arrays using random forest [PDF]
PeerJ, 2017 CRISPRs (clustered regularly interspaced short palindromic repeats) are particular repeat sequences found in wide range of bacteria and archaea genomes. Several tools are available for detecting CRISPR arrays in the genomes of both domains.
Kai Wang, Chun Liang
doaj +2 more sources
A method for cloning and sequencing long palindromic DNA junctions [PDF]
Nucleic Acids Research, 2004 DNA sequences containing long adjacent inverted repeats (palindromes) are inherently unstable and are associated with many types of chromosomal rearrangements. The instability associated with palindromic sequences also creates difficulties in their molecular analysis: long palindromes (>250 bp/arm) are highly unstable in Escherichia coli, and cannot be
openaire +3 more sources
A Mass Spectrometry Approach Reveals Fatty Acid Isomerism in Tomato Cold Tolerance
Advanced Science, EarlyView.An enhanced mass spectrometry approach reveals fatty acids (FAs) and FA isomers that are critical for tomato cold tolerance. Applying specific FA isomers can partially rescue cold sensitivity in tomato mutant plants, highlighting the importance of FA isomers in plant cold stress adaptation.
Leelyn Chong +7 more
wiley +1 more source
PPS: A computing engine to find Palindromes in all Protein sequences [PDF]
Bioinformation, 2014 The primary structure of a protein molecule comprises a linear chain of amino acid residues. Certain parts of this linear chain are unique in nature and function. They can be classified under different categories and their roles studied in detail. Two such unique categories are the palindromic sequences and the Single Amino Acid Repeats (SAARs), which ...
Manickam Gurusaran +6 more
openaire +3 more sources
Engineered Cas12j‐8 is a Versatile Platform for Multiplexed Genome Modulation in Mammalian Cells
Advanced Science, EarlyView.Engineered through structure‐guided protein engineering, enCas12j‐8 significantly enhances the editing efficiency of compact Cas12j‐8 while maintaining high specificity. It enables efficient multiplexed genome editing and base editing using a single crRNA array, demonstrating broad applicability and therapeutic potential in genome engineering ...
Ru Meng +14 more
wiley +1 more source
Advanced Cancer Immunotherapy via SMARCAL1 Blockade Using a Glucose‐Responsive CRISPR Nanovaccine
Advanced Science, EarlyView.A glucose‐catalyzed chemodynamic strategy is proposed to activate therapeutic genome editing of SMARCAL1to improve cancer immunotherapy. This innovative design featuring Fenton reaction activity and glucose consumption ability, not only effectively synergizes to activate the cGAS‐STING pathway but also inhibits the opposing functions of interferon by ...
Yuwei Li +8 more
wiley +1 more source
Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration [PDF]
Exploration of Drug ScienceAim: Evaluate the selective cytotoxic effect of the palindromic sequence RWQWRWQWR and its analogues obtained by replacement of L-amino acids by D-amino acids or the functionalization by adding the RGD (integrin ligand motif) to the peptide.
Andrea Barragán-Cárdenas +7 more
doaj +1 more source
Advanced Science, EarlyView.DSBs are the most critical genomic lesions. Using the ChromInSight framework, It is revealed that 3D chromatin structure, rather than linear distance, shapes cluster‐scene between hub‐Nodes and DSB loci. The “spatial isolation–damage containment” hypothesis is proposed to illustrate how the genome responds to genomic damage and offer a powerful tool ...
Kang Xu +9 more
wiley +1 more source
Advanced Science, EarlyView.This study investigates subgenome evolution and polyploidy in Cypriniformes fish, revealing complex phylogenetic relationships and genomic dynamics. By applying advanced subgenome partitioning methods, it uncovers novel insights into transposon activity and gene fractionation.
Yunyun Lv +14 more
wiley +1 more source