Results 81 to 90 of about 80,959 (317)

CRF: detection of CRISPR arrays using random forest [PDF]

PeerJ, 2017
CRISPRs (clustered regularly interspaced short palindromic repeats) are particular repeat sequences found in wide range of bacteria and archaea genomes. Several tools are available for detecting CRISPR arrays in the genomes of both domains.
Kai Wang, Chun Liang
doaj   +2 more sources

Chromatic quasisymmetric functions

, 2016
We introduce a quasisymmetric refinement of Stanley's chromatic symmetric function. We derive refinements of both Gasharov's Schur-basis expansion of the chromatic symmetric function and Chow's expansion in Gessel's basis of fundamental quasisymmetric ...
Shareshian, John, Wachs, Michelle L.
core   +1 more source

Mammalian Proteome Profiling Reveals Readers and Antireaders of Strand‐Symmetric and ‐Asymmetric 5‐Hydroxymethylcytosine‐Modifications in DNA

Advanced Science, EarlyView.
We investigate by proteomics studies how strand‐symmetric and ‐asymmetric cytosine 5‐modifications in DNA are selectively recognized by the nuclear proteome. Using promoter probes with defined modification patterns, we identify tissue‐specific reader proteinsincluding MYC, MAX, and RFX5that discriminate 5‐hydroxymethylcytosine symmetry and sequence ...
Lena Engelhard, Zeyneb Vildan Cakil, Marlon S. Zambrano‐Mila, Simone Eppmann, Tye Gonzalez, Rasmus Linser, Petra Janning, Sidney Becker, Daniel Summerer   +8 more
wiley   +1 more source

CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning

BioTechniques, 2015
Restriction enzymes have two major limitations for cloning: they cannot cleave at any desired location in a DNA sequence and may not cleave uniquely within a DNA sequence. In contrast, the clustered regularly interspaced short palindromic repeat (CRISPR)–
Jia-Wang Wang, Amy Wang, Kunyu Li, Bangmei Wang, Shunqian Jin, Michelle Reiser, Richard F Lockey   +6 more
doaj   +1 more source

TriCON: A Carbon‐Based Triple‐Modal Nanoplatform for Pancreatic Cancer Therapy

Advanced Science, EarlyView.
We developed TriCON, a triple‐modality nanotherapeutic platform, to treat pancreatic ductal adenocarcinoma (PDAC) by synergizing gene editing, chemotherapy, and immunotherapy. TriCON utilizes CRISPR/Cas9 to target the poliovirus receptor (PVR), combined with nano‐encapsulated doxorubicin and checkpoint blockade. This approach achieved significant tumor
Xinyu Peng, Jiaxing Huang, Shengnan Lv, Jian Zhang, Liangliang Zhang, Huan Liu, Yan Liu, Zhen‐An Qiao, Feng Wei, Bao‐Lian Su   +9 more
wiley   +1 more source

TGTT and AACA: two transcriptionally active LTR retrotransposon subfamilies with a specific LTR structure and horizontal transfer in four Rosaceae species

Mobile DNA, 2017
Background Long terminal repeat retrotransposons (LTR-RTs) are major components of plant genomes. Common LTR-RTs contain the palindromic dinucleotide 5′-‘TG’–‘CA’-3′ motif at the ends.
Hao Yin, Xiao Wu, Dongqing Shi, Yangyang Chen, Kaijie Qi, Zhengqiang Ma, Shaoling Zhang   +6 more
doaj   +1 more source

Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli. [PDF]

PLoS ONE, 2012
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (cas) genes conform the CRISPR-Cas systems of various bacteria and archaea and produce degradation of invading nucleic acids containing sequences (protospacers) that
Cristóbal Almendros, Noemí M Guzmán, César Díez-Villaseñor, Jesús García-Martínez, Jesús García-Martínez, Francisco J M Mojica   +5 more
doaj   +1 more source

Conjugacy Growth and Conjugacy Width of Certain Branch Groups [PDF]

, 2014
The conjugacy growth function counts the number of distinct conjugacy classes in a ball of radius $n$. We give a lower bound for the conjugacy growth of certain branch groups, among them the Grigorchuk group.
Fink, Elisabeth
core  

CRISPR in MOF Formulation with Enhanced Stability, Activity, and Altered PAM Specificity for Broad‐Spectrum Diagnosis of Bacterial Sepsis

Advanced Science, EarlyView.
CRISPR‐FLEXMO (CRISPR with flexible PAM in MOF encapsulation) integrates a PAM‐relaxed Cas12a K607R enzyme with a manganese‐coordinated MOF for broad‐spectrum diagnosis of bacterial sepsis. The MOF enhances activity and preserves functionality under heat, solvent, and chaotropic stress, supporting long‐term room‐temperature storage.
Tathagata Pal, Zilong Liu, Meera G. Nair, Juhong Chen   +3 more
wiley   +1 more source

The Power of CRISPR-Cas9-Induced Genome Editing to Speed Up Plant Breeding

International Journal of Genomics, 2016
Genome editing with engineered nucleases enabling site-directed sequence modifications bears a great potential for advanced plant breeding and crop protection.
Hieu X. Cao, Wenqin Wang, Hien T. T. Le, Giang T. H. Vu   +3 more
doaj   +1 more source