Results 171 to 180 of about 253,620 (220)

Pancreatic Elastase 1 after Pancreatic Transplantation

Pancreas, 1991
Human pancreatic elastase 1 (E1) is a novel pancreas-specific proteinase that has not yet been investigated after pancreatic transplantation (PTx). Using a recently developed E1 ELISA, we studied the E1 serum curves in 36 type I diabetic patients subjected to PTx with enteric exocrine diversion from the pretransplant value up to 8 years after ...
R, Linder, A, Sziegoleit, C, Brattström, G, Tydén, C G, Groth   +4 more
openaire   +2 more sources

Human cationic, pancreatic elastase in acute pancreatitis

Scandinavian Journal of Clinical and Laboratory Investigation, 1993
Immunoreactive cationic pancreatic elastase (irPE) in serum and peritoneal exudate from 8 patients with severe acute pancreatitis was found mainly in complex with alpha-1-proteinase-inhibitor (alpha 1PI). Only minor amounts of irPE were found in a molecular form corresponding to the free proenzyme. The presence of alpha-2-macroglobulin (alpha 2M)-bound,
H O, Håkansson, A, Borgström, K, Ohlsson   +2 more
openaire   +2 more sources

Digestion of elastin by porcine pancreatic elastase I and elastase II

International Journal of Peptide and Protein Research, 1985
Elastin was fully solubilized by digestion with elastase I or elastase II. Each digest was separated into high‐molecular weight and low‐molecular weight fractions that were characterized by the correspondence to their amino acid content, N‐terminal sequence and C‐terminal amino acids. It was found that although the relative amount of amino acids in the
M, Vered, Y, Burstein, A, Gertler
openaire   +2 more sources

Purification and characterization of ovine pancreatic elastase

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 1998
Elastase was isolated from ovine pancreas and purified to homogeneity by two different procedures. One involved precipitation with ammonium sulphate, p-aminobenzamidine-Sepharose chromatography, CM-Sepharose ion exchange chromatography and S-300 Sephacryl chromatography.
L S, Erlendsson, H, Filippusson
openaire   +2 more sources

Distribution of pancreatic elastase and metalloproteinase in vertebrates

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1986
Elastase-like enzymes were detected as zymogens in all of the pancreatic extracts from the gummy shark, bullhead shark, angel shark, smooth hammerhead, bestel, rainbow trout, carp, eel, Japanese mackerel, yellowtail, sea bass, parrotfish, bullfrog, chicken, bluewhite dolphin, hog, rat, cat, and dog.
R, Yoshinaka, M, Sato, N, Tsuchiya, S, Ikeda   +3 more
openaire   +2 more sources

Inhibition of Pancreatic Elastase by Polyphenolic Compounds

Journal of Agricultural and Food Chemistry, 2010
Polyphenols are plant secondary metabolites commonly present in the human diet that possess the ability to bind and inhibit digestive proteins. In the present study, kinetic measurements of porcine pancreatic elastase (PPE) activity were determined using Suc-(Ala)(3)-p-nitroanilide as substrate and polyphenolic compounds as inhibitors.
Natercia F Bras, Rui Goncalves, Nuno Mateus, Pedro A Fernandes, Maria Joao Ramos, Victor Do Freitas   +5 more
openaire   +3 more sources

Elastase Activity in Human Pancreatic Secretions

Nature, 1962
IN 1949, Balo and Banga1 first reported the isolation of the pancreatic enzyme elastase, which was unique in its ability to render soluble elastin (an important structural component of arteries, veins, skin and lung). This enzyme was first believed to be mucolytic in action, but it is now established that it is also a potent proteolytic agent2.
S, TINDEL, I J, SCHNEIDER, D, SHAPIRA, D, STATE   +3 more
openaire   +2 more sources

ELISA for human pancreatic elastase 1

Clinical Biochemistry, 1989
A sandwich-type ELISA for human pancreatic elastase 1 was developed and assessed for clinical feasibility. Using highly purified reagents, the test seems to be as sensitive and specific as the well-documented Radio-immunoassay (RIA) for the detection of elevated elastase 1 serum levels and, consequently, would have the same indications described for ...
A, Sziegoleit, H, Knäpler, B, Peters
openaire   +2 more sources

Stabilization of Lyophilized Porcine Pancreatic Elastase

Pharmaceutical Research, 1993
Porcine pancreatic elastase, a well-characterized serine protease, has been used as a model to assess the effects of excessive humidity on solid-state stability of the lyophilized protein. Elastase lyophilized without excipients retained full activity immediately after freeze-drying but became denatured upon continued storage at 40 degrees C, 75 ...
B S, Chang, C S, Randall, Y S, Lee
openaire   +2 more sources

Comparison of the Elastolytic Effects of Human Leukocyte Elastase and Porcine Pancreatic Elastase

Biochemical and Biophysical Research Communications, 1976
Porcine and bovine elastins were digested by human leukocyte elastase and porcine pancreatic elastase. The enzymes showed similarities in the extent to which they digested elastin and the pattern and quantitative distribution of N-terminal amino acids in the digests.
R M, Senior, D R, Bielefeld, B C, Starcher   +2 more
openaire   +3 more sources