Results 11 to 20 of about 319,790 (256)

Electrophoretic Separation on an Origami Paper-Based Analytical Device Using a Portable Power Bank

Sensors, 2019
The electrophoresis of ampholytes such as amino acids on a paper device is difficult because of the variation of pH distribution in time. On the basis of this observation, we propose a paper-based analytical device (PAD) with origami structure.
Yu Matsuda, Katsunori Sakai, Hiroki Yamaguchi, Tomohide Niimi   +3 more
doaj   +1 more source

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

Proteomes, 2020
Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus, Cécile Lelong, Thierry Rabilloud   +2 more
doaj   +1 more source

Separation of dog serum lipoproteins by ultracentrifugation, dextran sulfate precipitation, and paper electrophoresis*

Journal of Lipid Research, 1961
The separation of dog serum lipoproteins by ultracentrifugation at a density of 1.063 is hindered by the failure of the high density fraction to accumulate in the bottom portion of the centrifuge tube.
Toshio Sakagami, D.B. Zilversmit
doaj   +1 more source

Flash-comet assay

MethodsX, 2020
In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
doaj   +1 more source

Results of conducting the primary seed production using the method of electrophoresis in the FSBSI “ARC “Donskoy

Зерновое хозяйство России, 2022
The current paper has presented the study and estimation results of winter bread wheat varieties in primary seed production (2019-2021) based on the method of electrophoresis.
T. I. Firsova, M. M. Kopus, Yu. G. Skvortsova, G. A. Filenko   +3 more
doaj   +1 more source

Quantitative analysis of unconjugated and conjugated bile acids in duodenal fluid by densitometry after paper electrophoresis

Journal of Lipid Research, 1973
A new paper electrophoretic method for the separation of bile acids into five groups, (1) unconjugated, (2) glycine conjugates and (3) taurine conjugates, and (4) and (5) the respective monosulfates, is described.
Neil Kaplowitz, Norman B. Javitt
doaj   +1 more source

A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection [PDF]

Royal Society Open Science, 2018
A plug-in electrophoresis microchip for large-scale use aimed at improving maintainability with low fabrication and maintenance costs is proposed in this paper.
Mingpeng Yang, Zhe Huang, Hui You
doaj   +1 more source

Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis

Clinical Proteomics, 2011
Introduction The vast difference in the abundance of different proteins in biological samples limits the determination of the complete proteome of a cell type, requiring fractionation of proteins and peptides before MS analysis.
Seshi Beerelli, Raja Kumaraguru, Chandramouli KH   +2 more
doaj   +1 more source

Western blotting of native proteins from agarose gels

BioTechniques, 2022
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta   +10 more
doaj   +1 more source

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

Micromachines, 2021
In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm2 and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA ...
Jesús David Urbano-Gámez, Francisco Perdigones, José Manuel Quero   +2 more
doaj   +1 more source