Results 11 to 20 of about 512,921 (340)

Evaluation of gel chromatography for plasma lipoprotein fractionation

open access: yesJournal of Lipid Research, 1970
The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained.
Teizo Sata   +3 more
doaj   +1 more source

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

open access: yes, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi   +103 more
core   +5 more sources

Serum lipoproteins of normal and cholesterol-fed rats

open access: yesJournal of Lipid Research, 1973
The density distribution of lipoproteins in rats fed chow or chow containing 1% cholesterol and 10% olive oil was studied. Lipoprotein fractions were prepared in the ultracentrifuge between narrow density bands within the density range of 1.006–1.21 and ...
Norman L. Lasser   +3 more
doaj   +1 more source

Quantitation of serum lipoproteins by electrophoresis on agarose gel: standardization in lipoprotein concentration units (mg/100 ml) by comparison with analytical ultracentrifugation

open access: yesJournal of Lipid Research, 1971
Lipoprotein electrophoresis on agarose gel has been modified to allow estimation of the absolute quantity of each fraction. The reproducibility of the method is illustrated by 12 determinations in a single day on serum from one normal subject: mean total
S.B. HULLEY   +5 more
doaj   +1 more source

Electrophoretic Separation on an Origami Paper-Based Analytical Device Using a Portable Power Bank

open access: yesSensors, 2019
The electrophoresis of ampholytes such as amino acids on a paper device is difficult because of the variation of pH distribution in time. On the basis of this observation, we propose a paper-based analytical device (PAD) with origami structure.
Yu Matsuda   +3 more
doaj   +1 more source

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

open access: yesProteomes, 2020
Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus   +2 more
doaj   +1 more source

Separation of dog serum lipoproteins by ultracentrifugation, dextran sulfate precipitation, and paper electrophoresis*

open access: yesJournal of Lipid Research, 1961
The separation of dog serum lipoproteins by ultracentrifugation at a density of 1.063 is hindered by the failure of the high density fraction to accumulate in the bottom portion of the centrifuge tube.
Toshio Sakagami, D.B. Zilversmit
doaj   +1 more source

Flash-comet assay

open access: yesMethodsX, 2020
In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
doaj   +1 more source

Results of conducting the primary seed production using the method of electrophoresis in the FSBSI “ARC “Donskoy

open access: yesЗерновое хозяйство России, 2022
The current paper has presented the study and estimation results of winter bread wheat varieties in primary seed production (2019-2021) based on the method of electrophoresis.
T. I. Firsova   +3 more
doaj   +1 more source

DNA electrophoresis studied with the cage model [PDF]

open access: yes, 2001
The cage model for polymer reptation, proposed by Evans and Edwards, and its recent extension to model DNA electrophoresis, are studied by numerically exact computation of the drift velocities for polymers with a length L of up to 15 monomers.
A. van Heukelum   +25 more
core   +5 more sources

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