Results 21 to 30 of about 19,430,262 (258)

Western blotting of native proteins from agarose gels

open access: yesBioTechniques, 2022
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma   +10 more
doaj   +1 more source

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

open access: yesMicromachines, 2021
In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm2 and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA ...
Jesús David Urbano-Gámez   +2 more
doaj   +1 more source

Stochastic modeling for the COMET-assay [PDF]

open access: yes, 2003
We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. Essential is the use of point process structures, renewal theory and reduction to intensity histograms for further data ...
Hösel, V.   +4 more
core   +1 more source

Agarose Gel Analysis of 15–40-kb PCR Amplimers

open access: yesBioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland   +2 more
doaj   +1 more source

Application of capillary electrophoresis to amino acid sequencing of peptide

open access: yes, 2021
Separations of twenty phenylthiohydantoin (PTH) amino acids, and amino acid derivatives, resulting from the Edman degradation of peptides and proteins, were optimized for peptide sequencing by capillary electrophoresis.
이공주
core   +1 more source

Protein electrophoretic migration data from custom and commercial gradient gels

open access: yesData in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller   +2 more
doaj   +1 more source

Analysis of biopharma raw materials by electrophoresis microchips with contactless conductivity detection [PDF]

open access: yes, 2009
Detailed information concerning the composition of the raw materials employed in the production of biologics is important for the efficient control and optimization of bioprocesses.
Benito-Lopez, Fernando   +4 more
core   +3 more sources

20 Years of Fatty Acid Analysis by Capillary Electrophoresis

open access: yesMolecules, 2014
A review taking into account the literature reports covering 20 years of fatty acid analysis by capillary electrophoresis is presented. This paper describes the evolution of fatty acid analysis using different CE modes such as capillary zone ...
Marcone Augusto Leal de Oliveira   +6 more
doaj   +1 more source

A Novel Gaussian Extrapolation Approach for 2D Gel Electrophoresis Saturated Protein Spots [PDF]

open access: yes, 2012
Analysis of images obtained from two-dimensional gel electrophoresis (2D-GE) is a topic of utmost importance in bioinformatics research, since commercial and academic software available currently has proven to be neither completely effective nor fully ...
Caiazzo, A.   +7 more
core   +1 more source

Configurable on-Chip Gel Electrophoresis in Inkjet 3D Printed Microfluidic Modules

open access: yesProceedings, 2017
Inkjet 3D printing is with success used for preparation microfluidics devices as a microfluidic chips for DNA separation. Utilized this technic help us to prepare a chip in shorter time and cheaper, but length of microchannel are limited by possibility ...
Rafał Walczak   +2 more
doaj   +1 more source

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