Results 21 to 30 of about 319,790 (256)

Agarose Gel Analysis of 15–40-kb PCR Amplimers

BioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland, Foner P. Curtis, Noriko Kusukawa   +2 more
doaj   +1 more source

Protein electrophoretic migration data from custom and commercial gradient gels

Data in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller, Brandon Roman, Eric M. Norstrom   +2 more
doaj   +1 more source

20 Years of Fatty Acid Analysis by Capillary Electrophoresis

Molecules, 2014
A review taking into account the literature reports covering 20 years of fatty acid analysis by capillary electrophoresis is presented. This paper describes the evolution of fatty acid analysis using different CE modes such as capillary zone ...
Marcone Augusto Leal de Oliveira, Brenda Lee Simas Porto, Isaura Daniele Leite Faria, Patricia Lopes de Oliveira, Patricia Mendonça de Castro Barra, Renata de Jesus Coelho Castro, Renata Takabayashi Sato   +6 more
doaj   +1 more source

Configurable on-Chip Gel Electrophoresis in Inkjet 3D Printed Microfluidic Modules

Proceedings, 2017
Inkjet 3D printing is with success used for preparation microfluidics devices as a microfluidic chips for DNA separation. Utilized this technic help us to prepare a chip in shorter time and cheaper, but length of microchannel are limited by possibility ...
Rafał Walczak, Krzysztof Adamski, Wojciech Kubicki   +2 more
doaj   +1 more source

The design of mechatronic system for gel electrophoresis [PDF]

MATEC Web of Conferences, 2020
The paper present the methodology to design and implement a mechatronic system for gel electrophoresis. It describes the steps towards the development of a prototype and presents its functions. The new automated system is characterized by high precision,
Tsagaris Apostolos, Theodoridis Konstantinos, Stergiopoulos Fotis, Bechtsis Dimitrios, Nikolaidis Nikolaos, Triantafyllidis Dimitrios, Papaoikonomou Asterios, Filelis Anastasios   +7 more
doaj   +1 more source

Paper ionophoretic technique in the study of biologically important beryllium(II) / cobalt(II)-homoserine binary complexes in solution

Macedonian Journal of Chemistry and Chemical Engineering, 2010
Quantitative indication of the process of forming a complex comes from the evaluation of the stability constants, which characterize the equilibria corresponding to the successive addition of ligands.
Brij Bhushan Tewari
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Analysis of Dynamic Protein Expression Data

Revstat Statistical Journal, 2005
Difference gel electrophoresis (DIGE) is the new gold standard analysing complex protein mixtures in proteomics. It is used for measuring the expression levels of proteins in different mixtures on the same two-dimensional electrophoresis (2-DE) gel.
Klaus Jung , Ali Gannoun , Barbara Sitek , Helmut E. Meyer , Kai Stühler, Wolfgang Urfer   +5 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Capillary Electrophoresis as a Monitoring Tool for Flow Composition Determination

Molecules, 2021
Flow analysis is the science of performing quantitative analytical chemistry in flowing streams. Because of its efficiency and speed of analysis, capillary electrophoresis (CE) is a prospective method for the monitoring of a flow composition withdrawn ...
Mihkel Kaljurand, Piret Saar-Reismaa, Merike Vaher, Jelena Gorbatsova, Jekaterina Mazina-Šinkar   +4 more
doaj   +1 more source