FEBS Open Bio, EarlyView.We generated and characterized clear cell renal cell carcinoma models using the patient‐derived RCC243 cell line—including cell culture, orthotopic, and metastatic tumors—via single‐cell RNA‐sequencing for comparisons between models and patient tumor datasets.
Richard Huang +9 more
wiley +1 more source
BioTechniques, 2017 Protocol Summary Here we present an ethanol fixation protocol that allows for the direct visualization of the natural fluorescence of reporter proteins while maintaining excellent tissue histology.
Akifumi Nakagawa +5 more
doaj +1 more source
Formalin fixation and paraffin embedding interfere with the preservation of optical metabolic assessments based on endogenous NAD(P)H and FAD two-photon excited fluorescence. [PDF]
Biomed Opt Express, 2023 Sánchez-Hernández A +2 more
europepmc +1 more source
Localization of complement factor H gene expression and protein distribution in the mouse outer retina. [PDF]
, 2015 To determine the localization of complement factor H (Cfh) mRNA and its protein in the mouse outer retina.Quantitative real-time PCR (qPCR) was used to determine the expression of Cfh and Cfh-related (Cfhr) transcripts in the RPE/choroid.
Estep, Jason +6 more
core +1 more source
FEBS Open Bio, EarlyView.Liver‐specific knockout of N6‐methyladenosine (m6A) methyltransferase METTL3 significantly accelerated hepatic tumor initiation under various oncogenic challenges, contrary to the previously reported oncogenic role of METTL3 in liver cancer cell lines or xenograft models. Mechanistically, METTL3 deficiency reduced m6A deposition on Manf transcripts and
Bo Cui +15 more
wiley +1 more source
Xylene versus Isopropanol for Paraffin Wax Processing of Lung Tissue
Applied SciencesThe microscopic observation of lung tissue is challenging due to its fragile nature. Xylene and isopropanol are common tissue-clearing reagents used before paraffin embedding, yet no studies have compared these two reagents in lung tissue processing. Due
Qi Wang +4 more
doaj +1 more source
Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding. [PDF]
Acta Histochem Cytochem, 2022 Mizutani Y +6 more
europepmc +1 more source
PENGARUH KOMBINASI CYCLOPHOSPHAMIDE - TRANSFER FACTOR TERHADAP SKOR SEL T CD4+ PADA ADENOCARSINOMA MAMMAE MENCIT C3H [PDF]
, 2011 Background : Transfer factor are extracts from Colostrum, which is considered as an immunostimulant oligoribonukleotida for alternative therapies. Breast cancer patients will experience a decline in the immune system.
Shodiq, M Ali
core
The impact of frailty syndrome on skeletal muscle histology: preventive effects of exercise
FEBS Open Bio, EarlyView.Frailty syndrome exacerbates skeletal muscle degeneration via increased ECM deposition and myofiber loss. This study, using a murine model, demonstrates that endurance exercise attenuates these histopathological alterations, preserving muscle integrity. Findings support exercise as a viable strategy to counteract frailty‐induced musculoskeletal decline
Fujue Ji +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.In the blood–testis barrier, occludin is crucial for tight junctions. This study demonstrates that occludin‐targeting short peptides disrupt junction integrity, inducing immune cell infiltration, tumor necrosis factor‐α/interleukin‐6 secretion and mitochondrial dysfunction, ultimately triggering apoptosis.
Heng Wang, Xiaofang Tan, Deyu Chen
wiley +1 more source