Results 71 to 80 of about 1,356,440 (380)

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

Immobilization of urease in alginate, paraffin and lac [PDF]

Journal of the Serbian Chemical Society, 2010
The enzyme urease (EC.3.5.1.5) from jack bean meal was immobilized by various techniques, such as entrapment in calcium alginate gel spheres in aqueous suspension; lac impregnated muslin cloth as dry films and by embedding in paraffin wax impregnated ...
ANITA BAHADUR, NEELAM MISHRA, KESPI PITHAWALA   +2 more
doaj  

Immunohistochemical Detection of Intermediate Filament Nestin

Acta Medica, 1998
Using Rat-401 monoclonal antibody and peroxidase immunohistochemistry we have detected IF nestin in developing and adult rat tissues. Although epitope recognized by Rat-401 antibody is relatively resistant to aldehyde f ixation and paraffin embedding ...
Jaroslav Mokrý, Stanislav Němeček
doaj   +1 more source

Exploration of heterogeneity and recurrence signatures in hepatocellular carcinoma

Molecular Oncology, EarlyView.
This study leveraged public datasets and integrative bioinformatic analysis to dissect malignant cell heterogeneity between relapsed and primary HCC, focusing on intercellular communication, differentiation status, metabolic activity, and transcriptomic profiles.
Wen‐Jing Wu, Jianchao Wang, Fuqing Chen, Xuefeng Wang, Bin Lan, Ruyi Fu, Hong Wen, Fangfang Chen, Wei Hong, Tian‐Yu Tang, Ying He, Gang Chen, Jianyin Zhou, Hai‐Long Piao, Di Chen, Shu‐Yong Lin   +15 more
wiley   +1 more source

The p53 Mutation/Deletion Profile in a Small Cohort of the Omani Population with Diffuse Large B-Cell Lymphoma

Sultan Qaboos University Medical Journal, 2014
Objectives: Mutations/deletions affecting the TP53 gene are considered an independent marker predicting a poor prognosis for patients with diffuse large B-cell lymphoma (DLBCL).
Yahya Tamimi, Sheikha Al-Harthy, Ibrahim Al-Haddabi, Mohammed Al-Kindi, Hamza Babiker, Mansour Al-Moundhri, Ikram Burney   +6 more
doaj  

The atypical KRASQ22K mutation directs TGF‐β response towards partial epithelial‐to‐mesenchymal transition in patient‐derived colorectal cancer tumoroids

Molecular Oncology, EarlyView.
TGF‐β has a complex role in cancer, exhibiting both tumor‐suppressive and tumor‐promoting properties. Using a series of differentiated tumoroids, derived from different stages and mutational background of colorectal cancer patients, we replicate this duality of TGF‐β in vitro. Notably, the atypical but highly aggressive KRASQ22K mutation rendered early‐
Theresia Mair, Philip König, Milena Mijović, Jessica Kalla, Anil Baskan, Loan Tran, Kristina Draganić, Pedro Morata Saldaña, Carlos Uziel Pérez Malla, Janette Pfneissl, Andreas Tiefenbacher, Julijan Kabiljo, Velina S. Atanasova, Lisa Wozelka‐Oltjan, Leonhard Müllauer, Michael Bergmann, Raheleh Sheibani‐Tezerji, Gerda Egger   +17 more
wiley   +1 more source

Gene expression analysis in specific cell populations of bovine blastocysts using laser capture microdissection [PDF]

, 2012
Laser capture microdissection (LCM) has become a powerful technique for the isolation of specific cell populations from heterogeneous tissues for gene expression analysis.
Burvenich, Christian, Cornillie, Pieter, De Spiegelaere, Ward, De Spiegeleer, Bart, Goossens, Karen, Peelman, Luc, Stevens, Mieke, Van Soom, Ann   +7 more
core  

Targeting the AKT/mTOR pathway attenuates the metastatic potential of colorectal carcinoma circulating tumor cells in a murine xenotransplantation model

Molecular Oncology, EarlyView.
Dual targeting of AKT and mTOR using MK2206 and RAD001 reduces tumor burden in an intracardiac colon cancer circulating tumor cell xenotransplantation model. Analysis of AKT isoform‐specific knockdowns in CTC‐MCC‐41 reveals differentially regulated proteins and phospho‐proteins by liquid chromatography coupled mass spectrometry. Circulating tumor cells
Daniel J. Smit, Thais Pereira‐Veiga, Helena Brauer, Michael Horn, Paula Nissen, Thomas Mair, Bente Siebels, Hannah Voß, Ruimeng Zhuang, Marie‐Therese Haider, Desiree Loreth, Margarita Iskhakova, Bele Lindemann, Julian Kött, Laure Cayrefourcq, Jasmin Wellbrock, Hartmut Schlüter, Klaus Pantel, Catherine Alix‐Panabières, Manfred Jücker   +19 more
wiley   +1 more source

Experimental Research on Heating Transfer Improvement of Materials that Change Phase in Tunnels in Cold Regions

Geofluids, 2023
In tunnel heating systems, phase change materials may minimize the consumption of conventional electric energy, which is very considerate in the field of tunnel heating in cold regions.
Xu Zhang, Dianwei Qi, Wanjiang Wang, Jiaqi Zhang, Hongchao Yan, Xiancheng Li   +5 more
doaj   +1 more source

TmaDB: a repository for tissue microarray data [PDF]

, 2005
Background: Tissue microarray (TMA) technology has been developed to facilitate large, genome-scale molecular pathology studies. This technique provides a high-throughput method for analyzing a large cohort of clinical specimens in a single experiment ...
Quirke, P, Sharma-Oates, A, Westhead, DR
core   +3 more sources