Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Automated Machine Learning Strategies for Multi-Parameter Optimisation of a Caesium-Based Portable Zero-Field Magnetometer. [PDF]
Sensors (Basel), 2023 Dawson R +8 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Mathematical model of the process of converting waste oils into diesel biofuel
Наукові доповіді Національного університету біоресурсів і природокористування УкраїниThe growing demand for renewable energy sources encourages the development of technologies for the production of biodiesel from waste, but the high content of free fatty acids in raw materials complicates transesterification, reduces fuel yield, and ...
R. Chuiuk
doaj +1 more source
How generalisable are material extrusion additive manufacturing parameter optimisation studies? A systematic review. [PDF]
Heliyon, 2022 Golab M, Massey S, Moultrie J.
europepmc +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Slope Entropy Characterisation: An Asymmetric Approach to Threshold Parameters Role Analysis
EntropySlope Entropy (SlpEn) is a novel method recently proposed in the field of time series entropy estimation. In addition to the well-known embedded dimension parameter, m, used in other methods, it applies two additional thresholds, denoted as δ and γ, to ...
Mahdy Kouka +2 more
doaj +1 more source
Modelling and parameter optimisation for performance evaluation of sequencing batch reactor for treating hospital wastewater. [PDF]
Biomass Convers Biorefin, 2022 Khan NA +6 more
europepmc +1 more source
Type I interferons modulate autophagy to shape gemcitabine response in pancreatic cancer cells
FEBS Open Bio, EarlyView.Type I interferons differentially modulate autophagy and the response of pancreatic cancer cells to gemcitabine. IFNα2b stimulates autophagic flux and protects cells from gemcitabine‐induced cell death, contributing to chemoresistance. In contrast, IFNβ1a inhibits autophagosome formation and enhances gemcitabine‐induced cell death, resulting in ...
Lucy E. Bonilla +10 more
wiley +1 more source
Patient-Specific Modelling and Parameter Optimisation to Simulate Dilated Cardiomyopathy in Children. [PDF]
Cardiovasc Eng Technol, 2022 Bozkurt S +3 more
europepmc +1 more source