We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Regular Partial Differential Equations [PDF]
Birkhoff, Garrett, Mullikin, Thomas
openaire +2 more sources
Hamiltonian simulation for nonlinear partial differential equation by Schrödingerization. [PDF]
Sasaki S, Endo K, Muramatsu M.
europepmc +1 more source
Efficient Methods for Parameter Estimation of Ordinary and Partial Differential Equation Models of Viral Hepatitis Kinetics. [PDF]
Churkin A +4 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A modified coupled partial differential equation method for synthetic aperture sonar interferogram denoising. [PDF]
Huang P, Fan Y, Wang M, Guo L.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Modeling Metabolite Kinetics with a Partial Differential Equation-Based Hepatic Sinusoidal Model and MALDI-Mass Spectrometry Imaging of Verapamil and Its Metabolites. [PDF]
Parise RJ +5 more
europepmc +1 more source
A spatial partial differential equation approach to addressing unit misalignments in Bayesian poisson space-time models. [PDF]
Sumetsky N +3 more
europepmc +1 more source

