Results 71 to 80 of about 64,617 (266)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Particle‐Trapping Injection Flux Tubes in Saturn's Magnetosphere and High‐Band Electron Cyclotron Harmonic Waves Therein

Geophysical Research Letters
Injection flux tubes, characterized by localized equatorial magnetic field enhancements and concomitant hot plasma populations, contribute to Saturn's magnetospheric convection cycle by transporting magnetic flux radially inward.
Ze‐Fan Yin, Zi‐Shuo Ren, Xu‐Zhi Zhou, Yi‐Xin Sun, Sheng‐Yi Ye, Xin‐Ya Duanmu, Ze‐Jun Hu, Michel Blanc, Yi‐Xin Hao, Elias Roussos, Chao Yue, Qiu‐Gang Zong   +11 more
doaj   +1 more source

flux of a beam of particles

Citation: 'flux of a beam of particles' in the IUPAC Compendium of Chemical Terminology, 5th ed.; International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.09251 • License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International for ...
openaire   +1 more source

Hydrodynamic Energy Flux in a Many-Particle System

Physics Letters A
In this letter, using energy transfers, we demonstrate a route to thermalization in an isolated ensemble of realistic gas particles. We performed a grid-free classical molecular dynamics simulation of two-dimensional Lenard-Jones gas. We start our simulation with a large-scale vortex akin to a hydrodynamic flow and study its non-equilibrium behavior ...
Rauoof Wani, Mahendra Verma, Shashwat Nirgudkar, Sanat Tiwari   +3 more
openaire   +2 more sources

The Influence of a Charge on a Charged Particle Flux [PDF]

Australian Journal of Physics, 1963
The variations in intensity of a charged particle flux when observed from a position adjacent to a point charge have been calculated. This was done to estimate the effect of a Coulomb field on charged particles in space. It is shown that for repulsion a region of complete shadow occurs which has a width, at a particular radius, dependent on the ...
openaire   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Reduction of calcium flux from the extracellular region and endoplasmic reticulum by amorphous nano-silica particles owing to carboxy group addition on their surface

Biochemistry and Biophysics Reports, 2017
Several studies have reported that amorphous nano-silica particles (nano-SPs) modulate calcium flux, although the mechanism remains incompletely understood.
Akira Onodera, Katsutoshi Yayama, Hideto Morosawa, Yukina Ishii, Yasuo Tsutsumi, Yuichi Kawai   +5 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source