Results 71 to 80 of about 18,366,547 (391)

New tools for automated high-resolution cryo-EM structure determination in RELION-3

eLife, 2018
Here, we describe the third major release of RELION. CPU-based vector acceleration has been added in addition to GPU support, which provides flexibility in use of resources and avoids memory limitations.
J. Zivanov, T. Nakane, B. Forsberg, D. Kimanius, W. Hagen, E. Lindahl, S. Scheres   +6 more
semanticscholar   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Solution Structures of Engineered Vault Particles [PDF]

SSRN Electronic Journal, 2018
Prior crystal structures of the vault have provided clues of its structural variability but are non-conclusive due to crystal packing. Here, we obtained vaults by engineering at the N terminus of rat major vault protein (MVP) an HIV-1 Gag protein segment and determined their near-atomic resolution (∼4.8 Å) structures in a solution/non-crystalline ...
Ding, Ke, Zhang, Xing, Mrazek, Jan, Kickhoefer, Valerie A, Lai, Mason, Ng, Hwee L, Yang, Otto O, Rome, Leonard H, Zhou, Z Hong   +8 more
openaire   +5 more sources

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Correlates of successful structure solution in cryo-EM single-particle reconstruction (SPR) [PDF]

, 2021
Dominika Borek, Raquel Bromberg, Tabitha Emde, Yirui Guo, Daniel A. Plymire, Bradley J. Quade, Zbyszek Otwinowski   +6 more
openalex   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Scalar dark matter explanation of the excess in the Belle II B + → K ++ invisible measurement

Journal of High Energy Physics
Recently Belle II reported the first measurement of B + → K + + invisible(inv), which is 2.7σ above the standard model (SM) prediction. If confirmed, this calls for new physics beyond SM.
Xiao-Gang He, Xiao-Dong Ma, Michael A. Schmidt, German Valencia, Raymond R. Volkas   +4 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

SPICES: a particle-based molecular structure line notation and support library for mesoscopic simulation

Journal of Cheminformatics, 2018
Simplified Particle Input ConnEction Specification (SPICES) is a particle-based molecular structure representation derived from straightforward simplifications of the atom-based SMILES line notation.
Karina van den Broek, Mirco Daniel, Matthias Epple, Hubert Kuhn, Jonas Schaub, Achim Zielesny   +5 more
doaj   +1 more source

ENERGETIC PARTICLE DIFFUSION IN STRUCTURED TURBULENCE [PDF]

The Astrophysical Journal, 2012
In the full-orbit particle simulations of energetic particle transport in plasmas, the plasma turbulence is typically described as a homogeneous superposition of linear Fourier modes. The turbulence evolution is, however, typically a nonlinear process, and, particularly in the heliospheric context, the solar wind plasma is inhomogeneous due to the ...
Laitinen, Timo, Dalla, Silvia, Kelly, James   +2 more
openaire   +2 more sources