Results 201 to 210 of about 214,804 (267)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

open access: yesFEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow   +12 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

open access: yesFEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez   +6 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Patch testing in children

Contact Dermatitis, 1989
Over a 3-year period, 533 children aged between 4 months and 14 years were patch-tested with a “pediatric” series of haptens. The children were affected with various types of eczema or with psoriasis of the hands (n = 31). In 78 positive children, there were 112 positive test reactions to various allergens.
Balato N, Lembo G, Patruno C, Ayala F
openaire   +4 more sources

To patch or not to patch: What is your threshold for patch testing?

American Journal of Contact Dermatitis, 1999
Patch testing is as much art as it is science; we all are influenced by our clinical experience as well as by the literature. In an effort to assist those new to this often underutilized technique, we have solicited comments from five experienced clinicians about when to patch test and when not to patch test.
B A, Brod   +4 more
openaire   +2 more sources

Pitfalls in patch testing

American Journal of Contact Dermatitis, 2000
Patch testing is an invaluable diagnostic tool in the evaluation of allergic contact dermatitis. While TrueTest has simplified the technique for many practitioners, there remains potential for error. We asked 4 experts to describe their approach to several dilemmas encountered in patch testing.
D, Elston   +3 more
openaire   +2 more sources

Pitfalls in patch testing

Expert Review of Dermatology, 2010
Allergic contact dermatitis is a common skin disease affecting both genders and individuals of any age. The patch test is the only useful in vivo method for the etiological diagnosis of allergic contact dermatitis. It is an apparently simple technique, but pitfalls may be encountered.
Patruno C   +3 more
openaire   +3 more sources

Patch testing technique

Journal of the American Academy of Dermatology, 1989
The patch test is a necessary tool to evaluate contact dermatitis and other pruritic dermatoses. To obtain accurate test results, however, high-quality test material must be used, the application technique must be correct, and the results must be evaluated with skill and criticism.
T, Fischer, I, Kihlman
openaire   +2 more sources

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