Results 91 to 100 of about 2,978,757 (308)
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Natural space of the micro-object
Vestnik Samarskogo Gosudarstvennogo Tehničeskogo Universiteta. Seriâ: Fiziko-Matematičeskie Nauki, 2011 The immutability of classical dynamical laws for the microscopic object in the space which coordinate axes are the transition matrix elements of the corresponding coordinates is proved. It is stated that measurement is a micro-object localization process
Aleksey Yu Samarin
doaj
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
The functional measure for the in–in path integral [PDF]
Classical and Quantum Gravity, 2015 The in-in path integral of a scalar field propagating in a fixed background is formulated in a suitable function space. The free kinetic operator, whose inverse gives the propagators of the in-in perturbation theory, becomes essentially self adjoint after imposing appropriate boundary conditions.
openaire +3 more sources
Pathways and pitfalls: a qualitative study of student experiences in biomedical science education
FEBS Open Bio, EarlyView.Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell +8 more
wiley +1 more source
Path Integrals for (Complex) Classical and Quantum Mechanics
Acta Polytechnica, 2011 An analysis of classical mechanics in a complex extension of phase space shows that a particle in such a space can behave in a way redolent of quantum mechanics; additional dimensions permit ‘tunnelling’ without recourse to instantons and time/energy ...
R. J. Rivers
doaj
Vestnik Samarskogo Gosudarstvennogo Tehničeskogo Universiteta. Seriâ: Fiziko-Matematičeskie Nauki, 2014 A quantum path integral was transformed into the real form using a complex representation of the time. Such procedure gives the possibility to specify measures for the sets of the virtual paths in continual integrals determining amplitudes of quantum ...
Natalia V Meleshko, Alexey Yu Samarin
doaj +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley +1 more source
Visual Analysis of Cylindrically Polarized Light Beams’ Focal Characteristics by Path Integral
Open Physics, 2019 To analyze the focal characteristics of cylindrically polarized beams, a visual analysis method is proposed. As known, the focal field can be described by three mutually perpendicular components, each one is the total contribution of all parts of the ...
Yue Cheng-feng, Wu Yong-bo, Tang Zhi-lie
doaj +1 more source