Results 61 to 70 of about 196,292 (260)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Resting State Connectivity Between Medial Temporal Lobe Regions and Intrinsic Cortical Networks Predicts Performance in a Path Integration Task

Frontiers in Human Neuroscience, 2018
Humans differ in their individual navigational performance, in part because successful navigation relies on several diverse abilities. One such navigational capability is path integration, the updating of position and orientation during movement ...
Sarah C. Izen, Elizabeth R. Chrastil, Elizabeth R. Chrastil, Elizabeth R. Chrastil, Chantal E. Stern, Chantal E. Stern   +5 more
doaj   +1 more source

Effects of visual inputs on neural dynamics for coding of location and running speed in medial entorhinal cortex

eLife, 2020
Neuronal representations of spatial location and movement speed in the medial entorhinal cortex during the ‘active’ theta state of the brain are important for memory-guided navigation and rely on visual inputs.
Holger Dannenberg, Hallie Lazaro, Pranav Nambiar, Alec Hoyland, Michael E Hasselmo   +4 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Visual landmarks sharpen grid cell metric and confer context specificity to neurons of the medial entorhinal cortex

eLife, 2016
Neurons of the medial entorhinal cortex (MEC) provide spatial representations critical for navigation. In this network, the periodic firing fields of grid cells act as a metric element for position.
José Antonio Pérez-Escobar, Olga Kornienko, Patrick Latuske, Laura Kohler, Kevin Allen   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

How reduction of theta rhythm by medial septum inactivation may covary with disruption of entorhinal grid cell responses due to reduced cholinergic transmission

Frontiers in Neural Circuits, 2013
Oscillations in the coordinated firing of brain neurons have been proposed to play important roles in perception, cognition, attention, learning, navigation, and sensory-motor control.
Praveen K. Pilly, Stephen eGrossberg
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Models of grid cell spatial firing published 2005-2011

Frontiers in Neural Circuits, 2012
Since the discovery of grid cells in rat entorhinal cortex, many models of their hexagonally-arrayed spatial firing fields have been suggested. We review the models and organize them according to the mechanisms they use to encode position, update the ...
Eric A Zilli
doaj   +1 more source