Results 161 to 170 of about 3,727,441 (314)

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Peptide Aptamers: Innovative Design and Applications in Pathogen Detection. [PDF]

Chembiochem
Wu S, Wu X, Wen J, Chen J, Liu T, Gu D.
europepmc   +1 more source

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

FEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li, Miao Xin, Xiao‐wen Cui, Shi‐yin Yu, Fei Meng, Min Cao, Xiao‐hui Xiong, Yi‐chen Lu   +7 more
wiley   +1 more source

RNA Sequencing Resolves Cryptic Pathogenic Variants in Mitochondrial Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial diseases are the most common inherited metabolic disorders, characterized by pronounced clinical and genetic heterogeneity that complicates molecular diagnosis. Although DNA‐based sequencing approaches have become standard in genetic testing, up to half of patients remain without a definitive diagnosis.
Zhimei Liu, Xin Duan, Fatemeh Peymani, Jia Wang, Chengjia Bao, Chaolong Xu, Ying Zou, Zixuan Zhang, Yunxi Zhang, Tongyue Li, Martin Pavlov, Junling Wang, Minhan Song, Tianyu Song, Xiaodi Han, Mingxi Sun, Danmin Shen, Ruoyu Duan, Huafang Jiang, Manting Xu, Holger Prokisch, Fang Fang   +21 more
wiley   +1 more source

Hydrogel-Based Biosensors for Early Pathogen Detection

Bacterial plant pathogens are a major challenge to agricultural production, causing substantial crop and economic losses worldwide. In Canada, plant disease is a significant threat to valuable crops, and as Canada’s largest vegetable crop, potatoes ...
Roka Pringle, Allie Goodyear, Ramin Imran   +2 more
core   +1 more source

An Autonomous Pathogen Detection System for Assessing Temporal Associations with Respiratory Pathogen Activity. [PDF]

Microorganisms
Liu Y, Wang N, Wang Y, Zhao K, Li H, Yan Y, Li F, Yang R, Liu S, Xie Z, He Q, Niu Y, Hu K.   +12 more
europepmc   +1 more source

Understanding Further the Phenotypic Spectrum of Central Nervous System Inflammatory Demyelinating Disorders Using Unsupervised Clustering

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Central nervous system (CNS) inflammatory demyelinating syndromes, including multiple sclerosis (MS), aquaporin‐4 antibody–positive neuromyelitis optica spectrum disorder (AQP4 + NMOSD), and myelin oligodendrocyte glycoprotein (MOG) antibody–associated disease (MOGAD), occasionally overlap.
Bade Gulec, Elif Everest, Melih Tutuncu, Ugur Bilge, Sabahattin Saip, Ugur Uygunoglu, Aksel Siva   +6 more
wiley   +1 more source

Integrating Aggregate Materials and Machine Learning Algorithms: Advancing Detection of Pathogen‐Derived Extracellular Vesicles

Extracellular vesicles (EVs) are essential for host–pathogen interactions, mediating processes such as immune modulation and pathogen survival. Pathogen‐derived EVs hold significant diagnostic potential because of their unique cargo, offering a wealth of
Cong Hu, Tony Y. Hu, Yun Su, Liang Dong, Lihan Lai, Zehong Peng, Wei Xue   +6 more
core   +1 more source

Ofatumumab in Myelin Oligodendrocyte Glycoprotein Antibody–Associated Disease: A Comparison With Rituximab

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the efficacy and safety of ofatumumab in patients with myelin oligodendrocyte glycoprotein antibody–associated disease (MOGAD), and compare it with rituximab. Methods We conducted a single–center, observational study including 22 MOGAD patients treated with ofatumumab and 21 treated with rituximab.
Yuxin Fan, Lei Zhou, Jingzi ZhangBao, Hongmei Tan, Zhouzhou Wang, Chao Quan   +5 more
wiley   +1 more source

Complementary Use of Shotgun Metagenomics and Multiplex PCR for Pathogen Detection on High-Touch Surfaces. [PDF]

Microorganisms
Ghiba MTA, Alsheikh-Hussain A, Alzahrani TE, Alhamdoosh M, Alhetheel AF, Hanif A, Eifan SA.   +6 more
europepmc   +1 more source