Results 101 to 110 of about 455,752 (296)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

“Should I stay or should I go”—a kinase delays escape of Candida glabrata from macrophages

mBio
Candida glabrata is an opportunistic fungal pathogen that causes both superficial and systemic infections in humans, accounting for 15%–25% of invasive candidiasis cases. Macrophages play a crucial role in antifungal immunity by internalizing C. glabrata;
Theresa Lange, Colin Clairet, Luisa Fischer, Raghav Vij, Johannes Sonnberger, Julia Mantke, Nadja Jablonowski, Eric Seemann, Britta Qualmann, Christophe d’Enfert, Lydia Kasper, Bernhard Hube, Sascha Brunke   +12 more
doaj   +1 more source

Biofilms formed by Candida albicans bloodstream isolates display phenotypic and transcriptional heterogeneity that are associated with resistance and pathogenicity [PDF]

, 2014
Background: Candida albicans infections have become increasingly recognised as being biofilm related. Recent studies have shown that there is a relationship between biofilm formation and poor clinical outcomes in patients infected with biofilm ...
Borghi, Elisa, Falleni, Monica, Jones, Brian, Lappin, David F., Nile, Christopher, Perdoni, Federica, Rajendran, Ranjith, Ramage, Gordon, Sherry, Leighann, Smith, Karen, Tosi, Delfina, Williams, Craig   +11 more
core   +4 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses [PDF]

, 2013
In recent years, high pathogenicity avian influenza (HPAI) virus, H5N1, low pathogenicity avian influenza (LPAI) virus, H9N2, and both HPAI and LPAI H7 viruses have proved devastating for the affected economies reliant on poultry industry, and have posed
Böttcher-Frieberthäuser, Eva, Cattoli, Giovanni, Ferrara, Francesca, Molesti, Eleonora, Temperton, Nigel, Terregino, Calogero   +5 more
core   +3 more sources

The role of lipid metabolism in neuronal senescence

FEBS Open Bio, EarlyView.
Disrupted lipid metabolism, through alterations in lipid species or lipid droplet accumulation, can drive neuronal senescence. However, lipid dyshomeostasis can also occur alongside neuronal senescence, further amplifying tissue damage. Delineating how lipid‐induced senescence emerges in neurons and glial cells, and how it contributes to ageing and ...
Dikaia Tsagkari, Eleftheria Panagiotidou, Nektarios Tavernarakis   +2 more
wiley   +1 more source

Gene flow contributes to diversification of the major fungal pathogen Candida albicans

Nature Communications, 2018
The fungal pathogen Candida albicans can undergo a parasexual process that may contribute to genetic diversity, but its actual relevance is unclear. Here, Ropars et al. analyse the genomic sequences of 182 C.
Jeanne Ropars, Corinne Maufrais, Dorothée Diogo, Marina Marcet-Houben, Aurélie Perin, Natacha Sertour, Kevin Mosca, Emmanuelle Permal, Guillaume Laval, Christiane Bouchier, Laurence Ma, Katja Schwartz, Kerstin Voelz, Robin C. May, Julie Poulain, Christophe Battail, Patrick Wincker, Andrew M. Borman, Anuradha Chowdhary, Shangrong Fan, Soo Hyun Kim, Patrice Le Pape, Orazio Romeo, Jong Hee Shin, Toni Gabaldon, Gavin Sherlock, Marie-Elisabeth Bougnoux, Christophe d’Enfert   +27 more
doaj   +1 more source

Identification of a new intestinal spirochete with pathogenicity for chickens [PDF]

, 1995
Two intestinal spirochete isolates obtained from chickens with diarrhea were examined by electron microscopy, biochemical tests, rRNA gene restriction pattern analysis, and multilocus enzyme electrophoresis.
Swayne, D.E, Eaton, K.A., Stoutenburg, J., Trott, D.J., Hampson, D.J., Jensen, N.S.   +5 more
core   +1 more source

Salmonella Typhi, Paratyphi A, Enteritidis and Typhimurium core proteomes reveal differentially expressed proteins linked to the cell surface and pathogenicity [PDF]

, 2019
Background: Salmonella enterica subsp. enterica contains more than 2,600 serovars of which four are of major medical relevance for humans. While the typhoidal serovars (Typhi and Paratyphi A) are human-restricted and cause enteric fever, non-typhoidal ...
Barbé, Barbara, Deborggraeve, Stijn, Gevaert, Kris, Jacobs, Jan, Saleh, Sara, Staes, An, Timmerman, Evy, Van Puyvelde, Sandra   +7 more
core   +2 more sources

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source