Results 11 to 20 of about 154,598 (309)

Isolation of Human PBMCs

open access: yesBio-Protocol, 2013
Peripheral blood mononuclear cells (PBMCs) are chiefly lymphocytes and monocytes. PBMCs are separated from the whole blood by a density gradient centrifugation method using Ficoll Histopaque.
Santosh Panda, Balachandran Ravindran
doaj   +3 more sources

miR-146a-5p Promotes the Inflammatory Response in PBMCs Induced by Microcystin-Leucine-Arginine

open access: yesJournal of Inflammation Research, 2023
Huiying Zhang,1,* Daojun Chen,1,2,* Qianqian Ji,1 Meiyan Yang,1 Rui Ding1 1Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, 230032, People’s Republic of China; 2School of
Zhang H, Chen D, Ji Q, Yang M, Ding R
doaj   +1 more source

In vitro Culture of Human PBMCs

open access: yesBIO-PROTOCOL, 2013
Peripheral blood mononuclear cells (PBMCs) consist of chiefly of lymphocytes and monocytes. Purified PBMCs are used in vitro to evaluate a variety of functions of lymphocytes viz; a) proliferation to mitogenic (PHA, Con-A) stimulation, b) monitoring of prior sensitisation in antigen recall assays by scoring lymphocyte proliferation, c ...
Santosh Panda, Balachandran Ravindran
openaire   +3 more sources

Lack of evidence of mimivirus replication in human PBMCs [PDF]

open access: yesMicrobes and Infection, 2018
The Acanthamoeba polyphaga mimivirus (APMV) was first isolated during a pneumonia outbreak in Bradford, England, and since its discovery many research groups devoted efforts to understand whether this virus could be associated to human diseases, in particular clinical signs and symptoms of pneumonia.
Abrahão, Jônatas   +4 more
openaire   +4 more sources

PBMC- 01b Isolation of human PBMC from Whole Blood v4 [PDF]

open access: yes, 2020
Separation and purification of PBMC from FRESH BLOOD: list of published work using this protocol Kustrimovic, N., Comi, C., Magistrelli, L., Rasini, E., Legnaro, M., Bombelli, R., Aleksic, I., Blandini, F., Minafra, B., Riboldazzi, G., Sturchio, A., Mauri, M., Bono, G., Marino, F., & Cosentino, M. (2018).
Marco Cosentino   +6 more
  +7 more sources

Discovery of isomiRs in PBMCs of diseased vis-à-vis healthy Indian water buffaloes [PDF]

open access: yesExRNA, 2019
Background: MicroRNA isoforms are the variants of a canonical miRNA-sequence with alteration at the 3` or 5` termini due to addition, deletion and/or substitution of nucleotide. The present study aims at identifying the isomiRs of the expressed miRNAs in
Jasdeep Kaur Dhanoa   +5 more
doaj   +1 more source

Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons [PDF]

open access: yesBioImpacts
Introduction: Patient-derived induced pluripotent stem cells (iPSCs) have been widely used as disease models to test new therapeutic strategies. Moreover, the regenerative potential of stem cells can be improved with the use of biologically active ...
Duong Thi Thuy Le   +5 more
doaj   +1 more source

PBMC Isolation v2 [PDF]

open access: yes, 2017
Commonly used protocol to isolate peripheral blood mononuclear cells from whole human blood or apheresis ...
Girija Goyal, Jaclyn Long
openaire   +1 more source

Vaccination of monkey #2425 with autologous PBMCs.

open access: yes, 2023
Monkey #2425 was subcutaneously inoculated twice with 3×106 MMC-treated autologous CD8+ cell-depleted PBMCs that had been cultured for 3 days, with doses spaced 2 months apart. A, B.
Poonam Grover (14559966)   +13 more
core   +1 more source

PBMC Isolation v2 [PDF]

open access: yes, 2019
This protocol includes the step-by-step protocol used to isolate human peripheral blood mononuclear cells (PBMCs) from fresh human whole peripheral blood using the Ficoll density gradient method. This method seperated cells based on sedimentation velocity and cellular density.
Dannielle DK Moore, Andre G Loxton
openaire   +1 more source

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