Results 161 to 170 of about 769,066 (216)

Phytomycobiomes and Ecosystem Services: Mechanisms, Evidence and Routes to Application. [PDF]

open access: yesJ Fungi (Basel)
Ansari RA   +7 more
europepmc   +1 more source

Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis

open access: yesInternational Journal of Food Microbiology, 2003
The use of Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) technique in identifying the microorganisms present in commercial probiotic yoghurts and lyophilised products was evaluated. Two reference ladders were assembled constituted by PCR-amplified V2-V3 regions of 16S rDNA from bacterial species generally used as ...
Sandra Torriani   +2 more
exaly   +4 more sources

Effect of DNA polymerases on PCR-DGGE patterns

International Biodeterioration and Biodegradation, 2013
Abstract PCR-based molecular biological techniques became fundamental in the analysis of microbial communities. One of the most popular techniques is PCR-DGGE wherein the outcome is greatly influenced by the DNA amplification process. A large number of different factors were described as key elements for successful amplification, however the ...
Margit Balázs
exaly   +2 more sources

PCR-DGGE fingerprinting: novel strategies for detection of microbes in food

Journal of Microbiological Methods, 2004
Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed:
Danilo Ercolini
exaly   +4 more sources

Rapid identification of Armillaria species by PCR–DGGE

Journal of Microbiological Methods, 2014
The molecular approach has been used to distinguish between Armillaria species because of the ambiguity when using morphological traits. PCR-DGGE using the fungal primers ITS3GC-ITS4 enables a selective separation of amplicons and results in a simple and rapid approach to discriminate in one-step the most common Armillaria species in Europe.
Longa, Claudia Maria Oliveira   +1 more
openaire   +3 more sources

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