Results 1 to 10 of about 33,491 (200)

Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis. [PDF]

open access: yesAppl Environ Microbiol, 2011
ABSTRACT Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3′ ends of PCR primers, 16 primer pairs and fingerprints were generated per community.
Portillo MC   +3 more
europepmc   +5 more sources

Denaturing gradient gel electrophoresis as a diagnostic tool in periodontal microbiology. [PDF]

open access: yesJ Clin Microbiol, 2006
ABSTRACT Bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. The aim of the present study was to show the potential of denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant ...
Zijnge V   +5 more
europepmc   +7 more sources

Statistical analyses of complex denaturing gradient gel electrophoresis profiles. [PDF]

open access: yesJ Clin Microbiol, 2005
ABSTRACT Studies using molecular techniques have demonstrated that a culture-based approach can severely underestimate the bacterial diversity in most environments. One of the molecular techniques that has been applied in microbial ecology is denaturing gradient gel electrophoresis (DGGE).
Gafan GP   +5 more
europepmc   +5 more sources

Silver Staining of Denaturing Gradient Gel Electrophoresis Gels [PDF]

open access: yesClinical Chemistry, 2000
The continuing progress in the identification and characterization of genes that cause human genetic diseases has greatly increased the requests for a method that can rapidly identify mutations in these genes. The introduction of PCR has made it possible to rapidly obtain a relatively large amount of single-copy genomic DNA that can be used for ...
D, Radojkovic, J, Kusic
openaire   +4 more sources

Online Tool for Analysis of Denaturing Gradient Gel Electrophoresis Profiles [PDF]

open access: yesApplied and Environmental Microbiology, 2004
ABSTRACT We present an online tool (EquiBands, http://www.univie.ac.at/IECB/limno/equibands/EquiBands.html ) that quantifies the matching of two bands considered to be the same in different samples, even when samples are applied to different denaturing gradient gel electrophoresis gels.
Huber, Florian, Peduzzi, Peter
openaire   +2 more sources

Non-denaturing polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinemia [PDF]

open access: yesJournal of Lipid Research, 2003
Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterized by cholesterol-enriched VLDL that migrates in the beta-position on agarose gels. The demonstration of a broad beta-band on agarose gel electrophoresis of plasma is an insensitive method ...
Dirk J. Blom   +3 more
openaire   +3 more sources

Assessment of Bacterial Diversity of Industrial Poultry Wastewater by Denaturing Gradient Gel Electrophoresis (DGGE) and the Cultivation Method in Order to Inform Its Reuse in Agriculture. [PDF]

open access: yesBiomed Res Int, 2022
Oueslati A   +11 more
europepmc   +1 more source

Comparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis .pdf

open access: yes, 2020
Background: Candida species are usually opportunistic organisms that cause acute to chronic infections when conditions in the host are favorable. Accurate identification of Candida species is an essential pre-requisite for improved therapeutic strategy.
openaire   +1 more source

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