BioTechniques, 1998 Transgenic mice and cell lines are frequently developed to study human disease. Accurate determination of transgene copy number and levels of mRNA are necessary to understand the phenotypic changes observed in these models.
M.D. Ringel +4 more
doaj +1 more source
Supplementing in the diet of lactating Holstein cows may naturally produce coenzyme Q10-enriched milk [PDF]
Asian-Australasian Journal of Animal Sciences, 2018 Objective To examine the effects of Rhodobacter sphaeroides (R. sphaeroides) supplementation as a direct-fed microbial (DFM) on rumen fermentation in dairy cows and on coenzyme Q10 (CoQ10) transition into milk, an in vitro rumen simulation batch culture ...
Gui-Seck Bae +6 more
doaj +1 more source
Effect of bacterial inoculation, plant genotype and developmental stage on root-associated and endophytic bacterial communities in potato (Solanum tuberosum) [PDF]
, 2010 Beneficial bacteria interact with plants by colonizing the rhizosphere and roots followed by further spread through the inner tissues, resulting in endophytic colonization.
Overbeek, L.S., van +4 more
core +1 more source
Effects of short-term fasting on rumen microbiota and rumen fermentation characteristics [PDF]
Asian-Australasian Journal of Animal Sciences, 2019 Objective Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture ...
Jong Nam Kim +7 more
doaj +1 more source
Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis. [PDF]
J Clin Microbiol, 2011 We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing ...
Matussek A +6 more
europepmc +2 more sources
PHYLOGENETIC ANALYSIS OF BACTERIAL COMMUNITIES IN PANCURAN 7 BATURRADEN HOT SPRING [PDF]
, 2006 The diversity of the bacterial communities supported by culturing and capturing through 0.2 m-pore-size filter was studied. The Pancuran 7 hot spring has temperature at around 52°C and pH 7.
Mulyani, N.S. +5 more
core +1 more source
Microbial succession during thermophilic digestion: the potential of Methanosarcina sp.
PLoS ONE, 2014 A distinct succession from a hydrolytic to a hydrogeno- and acetotrophic community was well documented by DGGE (denaturing gradient gel electrophoresis) and dHPLC (denaturing high performance liquid chromatography), and confirmed by qPCR (quantitative ...
Paul Illmer +4 more
doaj +1 more source
Advances in Traceability Typing and Identification of Foodborne Pathogens
Shipin gongye ke-ji, 2022 Foodborne pathogens are the main factors affecting food quality and safety. Timely and accurate strain typing data enable rapid detection of outbreak clusters, informing ongoing infection control and public health responses, which are important for ...
Yao LIU +6 more
doaj +1 more source
Development of the preterm gut microbiome in twins at risk of necrotising enterocolitis and sepsis [PDF]
, 2013 The preterm gut microbiome is a complex dynamic community influenced by genetic and environmental factors and is implicated in the pathogenesis of necrotising enterocolitis (NEC) and sepsis.
Christopher J Stewart +37 more
core +1 more source
Cell Lysis Method Affects Assessment of Microbial Diversity Based on Ribotyping Analysis
Microbiology Indonesia, 2010 The microbial community in Kawah Hujan, Kamojang, West Java, Indonesia, was analyzed using 16S-rRNA-gene-sequencing combining with denaturing-gradient-gel electrophoresis (DGGE) technique.
HENI YOHANDINI +3 more
doaj +1 more source