Online Tool for Analysis of Denaturing Gradient Gel Electrophoresis Profiles [PDF]
Applied and Environmental Microbiology, 2004 ABSTRACT We present an online tool (EquiBands, http://www.univie.ac.at/IECB/limno/equibands/EquiBands.html ) that quantifies the matching of two bands considered to be the same in different samples, even when samples are applied to different denaturing gradient gel electrophoresis gels.
Huber, Florian, Peduzzi, Peter
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BioTechniques, 1998 Transgenic mice and cell lines are frequently developed to study human disease. Accurate determination of transgene copy number and levels of mRNA are necessary to understand the phenotypic changes observed in these models.
M.D. Ringel +4 more
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Supplementing in the diet of lactating Holstein cows may naturally produce coenzyme Q10-enriched milk [PDF]
Asian-Australasian Journal of Animal Sciences, 2018 Objective To examine the effects of Rhodobacter sphaeroides (R. sphaeroides) supplementation as a direct-fed microbial (DFM) on rumen fermentation in dairy cows and on coenzyme Q10 (CoQ10) transition into milk, an in vitro rumen simulation batch culture ...
Gui-Seck Bae +6 more
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Effects of short-term fasting on rumen microbiota and rumen fermentation characteristics [PDF]
Asian-Australasian Journal of Animal Sciences, 2019 Objective Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture ...
Jong Nam Kim +7 more
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Microbial succession during thermophilic digestion: the potential of Methanosarcina sp.
PLoS ONE, 2014 A distinct succession from a hydrolytic to a hydrogeno- and acetotrophic community was well documented by DGGE (denaturing gradient gel electrophoresis) and dHPLC (denaturing high performance liquid chromatography), and confirmed by qPCR (quantitative ...
Paul Illmer +4 more
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Advances in Traceability Typing and Identification of Foodborne Pathogens
Shipin gongye ke-ji, 2022 Foodborne pathogens are the main factors affecting food quality and safety. Timely and accurate strain typing data enable rapid detection of outbreak clusters, informing ongoing infection control and public health responses, which are important for ...
Yao LIU +6 more
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Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles [PDF]
Journal of Clinical Microbiology, 2005 ABSTRACT Studies using molecular techniques have demonstrated that a culture-based approach can severely underestimate the bacterial diversity in most environments. One of the molecular techniques that has been applied in microbial ecology is denaturing gradient gel electrophoresis (DGGE).
Gafan, G P +5 more
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Cell Lysis Method Affects Assessment of Microbial Diversity Based on Ribotyping Analysis
Microbiology Indonesia, 2010 The microbial community in Kawah Hujan, Kamojang, West Java, Indonesia, was analyzed using 16S-rRNA-gene-sequencing combining with denaturing-gradient-gel electrophoresis (DGGE) technique.
HENI YOHANDINI +3 more
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Spatiotemporal heterogeneity of plankton communities in Lake Donghu, China, as revealed by PCR-denaturing gradient gel electrophoresis and its relation to biotic and abiotic factors [PDF]
, 2008 The 16S and 18S rRNA genes of planktonic organisms derived from five stations with nutrient gradients in Lake Donghu, China, were studied by PCR-denaturing gradient gel electrophoresis (DGGE) fingerprinting, and the relationships between the genetic ...
Yan, Qingyun +3 more
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BioTechniques, 2000 PCR-based codominant genetic markers were developed by using primer sequences designed from cDNA clones of loblolly pine (Pinus taeda L.). Such markers offer certain advantages relative to simple-sequence repeat (SSR), also known as short-tandem repeat ...
Berhanu Temesgen +2 more
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