Analysis of the bacterial communities in the rhizosphere of Chrysanthemum via denaturing gradient gel electrophoresis of PCR- amplified 16S rRNA as well as DNA fragments coding for 16S rRNA [PDF]
The effect of developing chrysanthemum roots on the presence and activity of bacterial populations in the rhizosphere was examined by using culture-independent methods.
Anneke Keijzer +11 more
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Dysbetalipoproteinemia, an uncommon but highly atherogenic mixed hyperlipidemia due to the accumulation of remnants of triglyceride-rich lipoproteins, is characterized by cholesterol-enriched VLDL that migrates in the β-position on agarose gels.
Dirk J. Blom +3 more
doaj +1 more source
Double-Gradient DGGE for Optimized Detection of DNA Point Mutations
A novel technique is reported for screening point mutations in genomic DNA: double gradient, denaturing gradient gel electrophoresis (DG-DGGE). Unlike conventional DGGE, which exploits a single gradient of denaturing chemicals (typically urea and ...
L. Cremonesi +4 more
doaj +1 more source
The host genotype affects the bacterial community in the human gastrointestinal tract [PDF]
The gastrointestinal (GI) tract is one of the most complex ecosystems consisting of microbial and host cells. It is suggested that the host genotype, the physiology of the host and environmental factors affect the composition and function of the ...
Vos, W.M., de +4 more
core +1 more source
Accumulation of T cells carrying identical T-cell receptors (TCR) is associated with a number of immunological and nonimmunological diseases. Therefore, it is of interest to be able to analyze complex T-cell populations for the presence of clonally ...
P. thor Straten +5 more
doaj +1 more source
Paenibacillus brasilensis sp nov., a novel nitrogen-fixing species isolated from the maize rhizosphere in Brazil [PDF]
Sixteen nitrogen-fixing strains isolated from the rhizosphere of maize planted in Cerrado soil, Brazil, which showed morphological and biochemical characteristics similar to the gas-forming Paenibacillus spp., were phenotypically and genetically ...
Seldin, L. +3 more
core +1 more source
Epsilonproteobacteria in Humans, New Zealand
Using PCR–denaturing gradient gel electrophoresis, we examined 49 fecal samples from healthy volunteers and 128 diarrhea specimens to assess the distribution of Epsilonproteobacteria that might be routinely overlooked.
Angela J. Cornelius +5 more
doaj +1 more source
The effect of a multispecies probiotic on the composition of the faecal microbiota and bowel habits in chronic obstructive pulmonary disease patients treated with antibiotics [PDF]
Short-term antibiotic treatment profoundly affects the intestinal microbiota, which may lead to sustained changes in microbiota composition. Probiotics may restore such a disturbance.
Jonkers, D. +17 more
core +1 more source
Endophytic Colonization of Potato (Solanum tuberosum L.) by a Novel Competent Bacterial Endophyte, Pseudomonas putida Strain P9, and Its Effect on Associated Bacterial Communities [PDF]
Pseudomonas putida strain P9 is a novel competent endophyte from potato. P9 causes cultivar-dependent suppression of Phytophthora infestans. Colonization of the rhizoplane and endosphere of potato plants by P9 and its rifampin-resistant derivative P9R ...
Overbeek, L.S., van +23 more
core +1 more source
Application of Denaturing Gradient Gel ElectrophoresisApplication of Denaturing Gradient Gel Electrophoresis(DGGE) Methods on Parent-Offspring Relationship of the Coral Pocillopora damicornis [PDF]
DGGE (Denaturing Gradient Gel Electrophoresis) is the most powerful methods for mutation detection currentlyavailable. In DGGE, DNA fragments of the same length but with different sequences can be separated.
Permata W, Diah, Hirose, M, Hidaka , M
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