Results 11 to 20 of about 39,830 (289)

Comparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis .pdf

, 2020
Background: Candida species are usually opportunistic organisms that cause acute to chronic infections when conditions in the host are favorable. Accurate identification of Candida species is an essential pre-requisite for improved therapeutic strategy.
Parisa Mohammadi (6950810)
openaire   +2 more sources

Effect of vermicompost on changes in the bacterial community in maize rhizosphere [PDF]

Journal of Central European Agriculture, 2016
The aim of the study was to observe changes in the diversity of bacterial community in maize rhizosphere influenced by organic and mineral fertilization.
Eva HALENÁROVÁ, Juraj MEDO, Silvia KOVÁCSOVÁ, Ivana CHAROUSOVÁ, Jana MAKOVÁ, Jakub ELBL, Jaroslav ZÁHORA, Soňa JAVOREKOVÁ   +7 more
doaj   +1 more source

Denaturing Gradient Gel Electrophoresis (DGGE) Analysis Indicating Increased Microbial Diversity in Landfill Area Near Conserved Wetland [PDF]

Nature Environment and Pollution Technology, 2023
The ecological balance of an ecosystem has a relation to its biodiversity. Although it has been established that biodiversity and ecological stability are related, generalization about the exact nature of this relation remains elusive and more so in ...
M. B. Chakraborty, S. R. Patgiri, A. S. Rahman, A. Dasgupta and G. Pegu
doaj   +1 more source

Diversity of bacteria on fresh eggshell surface

Zhongguo shipin weisheng zazhi, 2020
Objective To understand the composition of microbiota on the surface of fresh eggshell and its change over time. Methods The samples were collected from three cities and tested at different time points.
Yi SHI, Wenjuan LI, IWei SH, Huan LI, Siruo ZHANG, Huan ZHANG, Dongli LIU, Guozhu MA   +7 more
doaj   +1 more source

Temperature-Programmed Capillary Electrophoresis for Detection of DNA Point Mutations

BioTechniques, 1996
A method for unambiguous determination of point mutations in genomic DNA, based on electrophoresis in thin capillaries, is reported here. The method is based on the principle of temperature gradient gel electrophoresis (TGGE), a variant of denaturing ...
Cecilia Gelfi, Laura Cremonesi, Maurizio Ferrari, Pier Giorgio Righetti   +3 more
doaj   +1 more source

Analysis of genetic diversity of Trypanosoma cruzi: an application of riboprinting and gradient gel electrophoresis methods

Memorias do Instituto Oswaldo Cruz, 2000
Analysis of restriction fragment length polymorphism (RFLP) profiles derived from digestion of polymerase chain reaction (PCR) products of the ribosomal 18S from Trypanosoma cruzi yields a typical `riboprint' profile that can vary intraspecifically.
JR Stothard, IA Frame, HJ Carrasco, MA Miles   +3 more
doaj   +1 more source

Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis. [PDF]

J Clin Microbiol, 2011
We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing ...
Matussek A, Stark L, Dienus O, Aronsson J, Mernelius S, Löfgren S, Lindgren PE.   +6 more
europepmc   +2 more sources

Double-Gradient DGGE for Optimized Detection of DNA Point Mutations

BioTechniques, 1997
A novel technique is reported for screening point mutations in genomic DNA: double gradient, denaturing gradient gel electrophoresis (DG-DGGE). Unlike conventional DGGE, which exploits a single gradient of denaturing chemicals (typically urea and ...
L. Cremonesi, S. Firpo, M. Ferrari, P.G. Righetti, C. Gelfi   +4 more
doaj   +1 more source

Epsilonproteobacteria in Humans, New Zealand

Emerging Infectious Diseases, 2012
Using PCR–denaturing gradient gel electrophoresis, we examined 49 fecal samples from healthy volunteers and 128 diarrhea specimens to assess the distribution of Epsilonproteobacteria that might be routinely overlooked.
Angela J. Cornelius, Stephen Chambers, John Aitken, Stephanie M. Brandt, Beverley Horn, Stephen L.W. On   +5 more
doaj   +1 more source

Detection and Characterization of αβ-T-Cell Clonality by Denaturing Gradient Gel Electrophoresis (DGGE)

BioTechniques, 1998
Accumulation of T cells carrying identical T-cell receptors (TCR) is associated with a number of immunological and nonimmunological diseases. Therefore, it is of interest to be able to analyze complex T-cell populations for the presence of clonally ...
P. thor Straten, A. Barfoed, T. Seremet, I. Saeterdal, J. Zeuthen, P. Guldberg   +5 more
doaj   +1 more source