Results 221 to 230 of about 85,409 (256)

Comprehensive typing of DQB1 alleles by PCR‐RFLP

Tissue Antigens, 1994
Abstract: The protocols represented in this report can resolve all 22 DQB1 alleles. The second exon of DQB1 was subjected to PCR using two group‐specific primers to obtain DQB1 group 1 (DQ5 and DQ6) and group 2 (DQ2, DQ3, DQ4) specific amplified products, respectively.
D P, Sengar, R, Goldstein
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Automated, PCR-RFLP Genotyping of the Urokinase Gene

Genetic Testing, 2000
The urokinase-type plasminogen activator (u-PA) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease (CAD) (Grenett et aL, 1998). Recently, a common genetic polymorphism in the untranslated region of the u-PA gene was shown to be associated with syptomatic CAD.
S M, Sell, F, Blasi, F, Booyse
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Identification of clinically relevant yeasts by PCR/RFLP

Journal of Microbiological Methods, 2004
For molecular diagnosis of fungal disease using DNA amplification procedures in the routine laboratory, choice of appropriate target structures and rapid and inexpensive identification of amplification products are important prerequisites. Most diagnostic procedures described thus far are characterized by limited applicability, considerable cost for ...
Anja, Trost, Barbara, Graf, Jan, Eucker, Orhan, Sezer, Kurt, Possinger, Ulf B, Göbel, Thomas, Adam   +6 more
openaire   +2 more sources

Discrimination of Penaeid Shrimps with PCR-RFLP Analysis

Journal of Shellfish Research, 2008
Abstract A rapid polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was designed to examine genetic differentiation of some penaeid shrimps. Three hundred and fifty-six bp of cytochrome-b gene, a specific part of mitochondrial genome, was amplified with PCR to figure out differences between shrimp species Penaeus ...
HİSAR, OLCAY, Hisar, Sukriye Aras, Mol, Suhendan, AKSAKAL, ERCÜMENT, YANIK, TELAT   +4 more
openaire   +3 more sources

Molecular characterization of potato virus YN isolates by PCR-RFLP

European Journal of Plant Pathology, 1996
Based on the sequence polymorphism in the 5′ terminal part of the viral genome, a range of PVYN isolates were characterized by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). Three pairs of primers selected in the 5′ non-translated and P1 protein region were tested.
Glais, L., Kerlan, Camille, Tribodet, Michel, Marie-Jeanne, Véronique, Robaglia, Christophe, Astier-Manifacier, S.   +5 more
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PCR, RFLP

2003
Tehnika umnažanja DNA, lančana reakcija polimerazom (PCR), revolucionarna je metoda današnjice. Metoda omogućava eksponencijalno umnažanje ciljnog dijela DNA u uvjetima in vitro. Pokazala se najspecifičnijom i najosjetljivijom za molekularno-genetičke analize. Postoje različiti pristupi samog umnažanja: 1. jednostruki PCR, 2. višestruki PCR, 3.
Grce, Magdalena, Matovina, Mihaela, Husnjak, Koraljka, Milutin, Nina   +3 more
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Differentiation of sturgeon species by PCR-RFLP

Food Research International, 1999
Abstract A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction ...
Christian Wolf, Philipp Hübner, Jürg Lüthy   +2 more
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Differentiation of H. pylori Strains Using PCR RFLP

2003
Helicobacter pylori strains have been shown to display considerable heterogeneity with respect to DNA sequence. Diverse restriction fragment length polymorphism (RFLP) patterns are generated among strains by restriction endonuclease digestion of whole chromosomal DNA (1-3), digestion of specific polymerase chain reaction (PCR) products (4), or ...
H L, Mobley, P A, Foxall
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Diagnosis of Penicillin Resistance by PCR-RFLP

2003
Streptococcus pneumoniae is an important human pathogen causing a wide spectrum of disease including pneumonia, otitis media, bacteraemia, and meningitis. It is a significant cause of morbidity and mortality worldwide and now penicillin resistance is becoming an ever increasing problem (1-2). Initially, all S.
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Human platelet antigen‐1 (Zw) typing using PCR‐RFLP

Transfusion Medicine, 1993
Summary. The agreement between human platelet antigen‐1 typing with polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) and typing with a serological ELISA method was evaluated. A total of 82 individuals were typed and an absolute correlation was found between the two typing methods.
B R, Andersen, J, Georgsen, H O, Madsen, E, Taaning, N, Grunnet, A, Svejgaard   +5 more
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