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Collaborative exploration of genetic resources of papaya and mountain papayas for their imprrovement and promotion [PDF]
Caetano, Creuci Maria+21 more
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PCR-RFLP for Aspergillus Species [PDF]
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is the most simple method for single-nucleotide change detection. It is widely used in the detection and differentiation between mycotoxigenic species. It is based on PCR amplification of a target region containing the variant site of the studied species followed by ...
Ali Atoui, Ali Atoui, André El Khoury
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Molecular characterization of potato virus YN isolates by PCR-RFLP
European Journal of Plant Pathology, 1996Based on the sequence polymorphism in the 5′ terminal part of the viral genome, a range of PVYN isolates were characterized by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). Three pairs of primers selected in the 5′ non-translated and P1 protein region were tested.
Glais, L.+5 more
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ABO genotyping by PCR-RFLP and cloning and sequencing
Anthropologischer Anzeiger, 2004A refined PCR-RFLP based method was established to genotype ABO blood groups. The main objective of this study was to make the techniques also suitable for working with degraded DNA. Specific primer design was carried out to choose fragments shorter than 200 bp as necessary in forensic and archaeological applications.
Haak, W., Burger, J., Alt, K.
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Differentiation of sturgeon species by PCR-RFLP
Food Research International, 1999Abstract A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction ...
Philipp Hübner+2 more
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Discrimination of Penaeid Shrimps with PCR-RFLP Analysis
Journal of Shellfish Research, 2008Abstract A rapid polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was designed to examine genetic differentiation of some penaeid shrimps. Three hundred and fifty-six bp of cytochrome-b gene, a specific part of mitochondrial genome, was amplified with PCR to figure out differences between shrimp species Penaeus ...
HİSAR, OLCAY+4 more
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Comprehensive typing of DQB1 alleles by PCR‐RFLP
Tissue Antigens, 1994Abstract: The protocols represented in this report can resolve all 22 DQB1 alleles. The second exon of DQB1 was subjected to PCR using two group‐specific primers to obtain DQB1 group 1 (DQ5 and DQ6) and group 2 (DQ2, DQ3, DQ4) specific amplified products, respectively.
Rose Goldstein, Dharmendra P.S. Sengar
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Identification of common species of dermatophytes by PCR-RFLP
Journal of Huazhong University of Science and Technology [Medical Sciences], 2005To establish a simple, sensitive and effective technique for the identification of six common dermatophytes, polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) targeting Topoisomerase II gene were used. The DNA of 6 common dermatophytes was amplified by primer dPsD1 and then primers dPsD2.
He Ganlin+3 more
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Automated, PCR-RFLP Genotyping of the Urokinase Gene
Genetic Testing, 2000The urokinase-type plasminogen activator (u-PA) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease (CAD) (Grenett et aL, 1998). Recently, a common genetic polymorphism in the untranslated region of the u-PA gene was shown to be associated with syptomatic CAD.
F. Booyse, Susan M. Sell, F. Blasi
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