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การตรวจวินิจฉัยแยกชนิดเชื้อบิดในไก่ด้วยวิธี PCR-RFLP
โรคบิดในไก่เป็นโรคโปรโตซัวมีสาเหตุมาจากเชื้อบิดในสกุล Eimeria โดยทั่วไปพื้นฐานของการตรวจแยกชนิดเชื้อบิดในไก่เป็นการแยกจากลักษณะรูปร่างของเชื้อบิดและบริเวณตำแหน่งในลำไส้ของไก่ที่ติดเชื้อบิด ซึ่งจากหลักการนี้พบว่าการตรวจแยกความแตกต่างชนิดของเชื้อบิดในบางครั้งให้ผลไม่น่าเชื่อถือ จึงได้มีการพัฒนานำวิธี Polymerase Chain Reaction-Restriction Fragment Length ...openaire +1 more source
Identification of common species of dermatophytes by PCR-RFLP
Journal of Huazhong University of Science and Technology [Medical Sciences], 2005To establish a simple, sensitive and effective technique for the identification of six common dermatophytes, polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) targeting Topoisomerase II gene were used. The DNA of 6 common dermatophytes was amplified by primer dPsD1 and then primers dPsD2.
Ganlin, He +3 more
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A PstI PCR-RFLP at the goat CSN1S2 gene.
Animal genetics, 1999By means of PCR the DNA region spanning from the 17th to the 18th exon of the goat CSN1S2 gene was amplified. PCR products were digested with PstI endonuclease. Digestion products showed a triallelic polymorphism (A, B and C). The frequencies of A, B and C alleles in 155 goats of undefined type reared in Southern Italy were 0.31, 0.40 and 0.29 ...
RAMUNNO, LUIGI +5 more
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HLA‐DRB5 genotyping by PCR‐RFLP
Tissue Antigens, 1994J, Trejaut, H, Dunckley
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[PCR-RFLP analysis of the genus Saccharomonospora].
Wei sheng wu xue bao = Acta microbiologica Sinica, 2004PCR-RFLP, a molecular method of polyphasic taxonomy of the genus Saccharomonospora was introduced. The feasibility of the application of this method at the species level in the taxonomy and identification of Saccharomonospora were discussed. The results showed that PCR-RFLP analysis is valuable for the determination of taxonomic position in the genus ...
Z, Lu, Z, Liu
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