Results 241 to 250 of about 82,842 (255)
Some of the next articles are maybe not open access.
Identification of clinically relevant yeasts by PCR/RFLP
Journal of Microbiological Methods, 2004For molecular diagnosis of fungal disease using DNA amplification procedures in the routine laboratory, choice of appropriate target structures and rapid and inexpensive identification of amplification products are important prerequisites. Most diagnostic procedures described thus far are characterized by limited applicability, considerable cost for ...
Thomas Adam +6 more
openaire +3 more sources
PCR-RFLP analysis of cpDNA in the genus Abies
Theoretical and Applied Genetics, 1999We used PCR-RFLP analysis of the chloroplast DNA of the genus Abies (family Pinaceae), to determine if the method could be employed to detect inter-specific variation in this genus and to study how the variation was distributed in different regions of the genome.
Parducci Laura, Szmidt Alfred E
openaire +2 more sources
A rapid PCR–RFLP method for the identification of Lophius species
European Food Research and Technology, 2012The seven Anglerfish species, which belong to the genus Lophius, have a different value on the market, worldwide. If whole fishes can be identified by their morphological characteristics, they become indistinguishable when prepared or processed. In this study, a rapid method based on polymerase chain reaction–restriction fragment length polymorphism ...
ARMANI, ANDREA +5 more
openaire +3 more sources
Differentiation of anaerobic polycentric fungi by rDNA PCR-RFLP [PDF]
Folia Microbiologica, 2006 The suitability of restriction fragment length polymorphism (RFLP) analysis of the ribosomal DNA cluster for discriminating two genera of anaerobic polycentric fungi, Orpinomyces and Anaeromyces, was determined. Three PCR-amplified DNA fragments--nuclear small subunit (SSU; 18S rDNA), the nuclear large subunit (LSU; 28S rDNA) and internal transcribed ...
Jakub Mrázek +2 more
openaire +2 more sources
Diagnosis of Penicillin Resistance by PCR-RFLP
2003Streptococcus pneumoniae is an important human pathogen causing a wide spectrum of disease including pneumonia, otitis media, bacteraemia, and meningitis. It is a significant cause of morbidity and mortality worldwide and now penicillin resistance is becoming an ever increasing problem (1-2). Initially, all S.
openaire +3 more sources
PCR- RFLP for Identification of Bluetongue Virus Serotypes
Southwestern Entomologist, 2015Abstract. Bluetongue virus is the etiological agent of a disease characterized by bleeding in domestic and wild ruminants. Different viral strains can infect a ruminant species and severity of infection is variable. Transmission of the virus under natural conditions is through the bite of a mosquito of the genus Culicoides (Ceratopogonidae), but also ...
Rafael González-Alvarez +12 more
openaire +2 more sources
Diversity of soil fungi studied by PCR-RFLP of ITS
Mycological Research, 2000Ribosomal DNA sequences provide new molecular approaches to characterise fungal species from the environment. This study presents a culture-independent approach to assessing fungal diversity by direct amplification, cloning, restriction digestion and sequencing of internal transcribed spacers (ITS).
Viaud, Muriel +2 more
openaire +3 more sources
Differentiation of H. pylori Strains Using PCR RFLP
2003Helicobacter pylori strains have been shown to display considerable heterogeneity with respect to DNA sequence. Diverse restriction fragment length polymorphism (RFLP) patterns are generated among strains by restriction endonuclease digestion of whole chromosomal DNA (1-3), digestion of specific polymerase chain reaction (PCR) products (4), or ...
Harry L. T. Mobley, Paul A. Foxall
openaire +3 more sources
HLA‐DRB5 genotyping by PCR‐RFLP
Tissue Antigens, 1994Heather Dunckley, Jean A Trejaut
openaire +2 more sources