Results 251 to 260 of about 3,745,037 (345)

Dating PCR [PDF]

Nature Biotechnology, 1990
openaire   +2 more sources

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Multimodal deep learning for predicting neoadjuvant treatment outcomes in breast cancer: a systematic review. [PDF]

Biol Direct
Krasniqi E, Filomeno L, Arcuri T, Ferretti G, Gasparro S, Fulvi A, Roselli A, D'Onofrio L, Pizzuti L, Barba M, Maugeri-Saccà M, Botti C, Graziano F, Puccica I, Cappelli S, Pelle F, Cavicchi F, Villanucci A, Paris I, Calabrò F, Rea S, Costantini M, Perracchio L, Sanguineti G, Takanen S, Marucci L, Greco L, Kayal R, Moscetti L, Marchesini E, Calonaci N, Blandino G, Caravagna G, Vici P.   +33 more
europepmc   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

Methodology and application of multiplex PCR-dipstick DNA chromatography for the detection of eight respiratory bacterial pathogens. [PDF]

Front Cell Infect Microbiol
Hu L, Wang X, Li Q.
europepmc   +1 more source

Co‐overexpression of the caloric restriction‐induced mitochondrial factors PGC‐1α and MIPEP upregulates Phospho1 expression in adipocytes

FEBS Open Bio, EarlyView.
Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu, Kanari Taki, Asuka Hayami, Komachi Kato, Yuka Nozaki, Yuhei Mizunoe, Takumi Narita, Ryoichi Mori, Yoshikazu Higami, Masaki Kobayashi   +9 more
wiley   +1 more source

LINC00323 variant is associated with increased risk of essential tremor

Annals of Clinical and Translational Neurology, EarlyView.
Abstract Essential tremor (ET) is a common adult movement disorder, with accumulating evidence suggesting that genetic factors primarily account for ET risk. However, replication studies on the genetic variants have yielded inconsistent results. In our case–control study, we show that the LINC00323 variant, identified in a European GWAS study, is ...
Brendan Tan, Ebonne Ng, Qiao Yang Sun, Aaron Mai, Ling‐Ling Chan, Thomas Welton, Kumar M. Prakash, Kay Yaw Tay, Wing Lok Au, Louis C.S. Tan, Eng‐King Tan, Bin Xiao   +11 more
wiley   +1 more source

A Highly Sensitive Multiplex Antibody Assay Reduces Window Period for Detection of <i>Babesia microti</i> Infection. [PDF]

Open Forum Infect Dis
Meredith S, Puri A, Majam VF, Zheng H, Oakley MS, Tonnetti L, Stramer SL, Kumar S.   +7 more
europepmc   +1 more source

A Novel CHMP2B Splicing Variant in Atypical Presentation of Familial Frontotemporal Lobar Degeneration

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT C‐truncating variants in the charged multivesicular body protein 2B (CHMP2B) gene are a rare cause of frontotemporal lobar degeneration (FTLD), previously identified only in Denmark, Belgium, and China. We report a novel CHMP2B splice‐site variant (c.35‐1G>A) associated with familial FTLD in Spain. The cases were two monozygotic male twins who
Sara Rubio‐Guerra, Sara Bernal, David Almenta, Josefina Pérez‐Blanco, Valle Camacho, Isabel Sala, Mª Belén Sánchez‐Saudinós, Jesús García Castro, Judit Selma‐González, Miguel Ángel Santos‐Santos, Álvaro Carbayo, Janina Turon‐Sans, Ricard Rojas‐Garcia, Daniel Alcolea, Juan Fortea, Alberto Lleó, Oriol Dols‐Icardo, Ignacio Illán‐Gala   +17 more
wiley   +1 more source

Establishment of Cre/LoxP-mediated multifunctional reporter knock-in rats with the CRISPR system. [PDF]

PLoS One
Nakamura K, Ito S, Ohguchi Y, Jimbo T, Wada Y, Nakajima R, Kanou M, Yamana K, Ueda H.   +8 more
europepmc   +1 more source