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ExCyto PCR amplification. [PDF]

open access: yesPLoS ONE, 2010
ExCyto PCR cells provide a novel and cost effective means to amplify DNA transformed into competent bacterial cells. ExCyto PCR uses host E. coli with a chromosomally integrated gene encoding a thermostable DNA polymerase to accomplish robust, hot-start ...
Vinay Dhodda   +9 more
doaj   +5 more sources

Reduction of heteroduplex formation in PCR amplification

open access: yesBiologia Plantarum, 2010
Heteroduplex formation is known to occur during mixed-template polymerase chain reaction (PCR) using universal primers, and may represent a serious problem in several PCR-based analyses.
E. Michu   +3 more
doaj   +3 more sources

Optimization of PCR amplification of maize microsatellite loci [PDF]

open access: yesGenetics and Molecular Biology, 2000
Maize (Zea mays L.) microsatellite loci are useful as genetic markers because they are numerous, occur in every chromosome, and have a high content of polymorphism information.
Ogliari Juliana Bernardi   +2 more
doaj   +5 more sources

Evaluation of the genetic relatedness of Bacteroides fragilis isolates by TRs analysis [PDF]

open access: yesIranian Journal of Basic Medical Sciences, 2020
Objective(s): Human gastrointestinal tract harbors a variety of bacteria with vital roles in human health. Bacteroides fragilis is considered one of the dominant constituents of gut microflora which can act as an opportunistic pathogen leading to various
Niloofar Khodaei   +7 more
doaj   +1 more source

Identification of S-Allele Based Self-incompatibility of Turkish Pear Gene Resources

open access: yesJournal of Agricultural Sciences, 2022
Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus (S-allele) has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple ...
Merve Dilek Karataş   +6 more
doaj   +1 more source

Screening of highly efficient photosynthetic hybrids of Oryza officinalis and analysis of their photosynthetic pathway genes

open access: yesPhotosynthetica, 2021
Hereditary properties of strong growth and huge accumulation of biomass in Oryza officinalis exhibit a great potential; however, the genes that code for its high photosynthesis performance are not established.
E.X. LI   +7 more
doaj   +1 more source

Comparison of methods to extract PCR-amplifiable DNA from fruit, herbal and black teas

open access: yesCzech Journal of Food Sciences, 2021
The success of polymerase chain reaction (PCR) assay depends on template deoxyribonucleic acid (DNA) being sufficient with respect to both quantity and quality.
Eliška Čermáková   +3 more
doaj   +1 more source

An evaluation of direct PCR amplification [PDF]

open access: yesCroatian Medical Journal, 2014
To generate complete DNA profiles from blood and saliva samples deposited on FTA® and non-FTA® paper substrates following a direct amplification protocol.Saliva samples from living donors and blood samples from deceased individuals were deposited on ten different FTA® and non-FTA® substrates.
Daniel E. Hall, Reena Roy
openaire   +3 more sources

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