Results 1 to 10 of about 1,169,901 (341)

ExCyto PCR amplification. [PDF]

open access: yesPLoS ONE, 2010
ExCyto PCR cells provide a novel and cost effective means to amplify DNA transformed into competent bacterial cells. ExCyto PCR uses host E. coli with a chromosomally integrated gene encoding a thermostable DNA polymerase to accomplish robust, hot-start ...
Vinay Dhodda   +9 more
doaj   +4 more sources

Primer design versus PCR bias in methylation independent PCR amplifications [PDF]

open access: hybridEpigenetics, 2009
Many protocols in methylation studies utilize one primer set to generate a PCR product from bisulfite modified template regardless of its methylation status (methylation independent amplification MIP). However, proportional amplification of methylated and unmethylated alleles is hard to achieve due to PCR bias favoring amplification of unmethylated ...
Tomasz K. Wojdacz   +2 more
openalex   +4 more sources

Identification of S-Allele Based Self-incompatibility of Turkish Pear Gene Resources

open access: yesJournal of Agricultural Sciences, 2022
Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus (S-allele) has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple ...
Merve Dilek Karataş   +6 more
doaj   +1 more source

Evaluation of the genetic relatedness of Bacteroides fragilis isolates by TRs analysis [PDF]

open access: yesIranian Journal of Basic Medical Sciences, 2020
Objective(s): Human gastrointestinal tract harbors a variety of bacteria with vital roles in human health. Bacteroides fragilis is considered one of the dominant constituents of gut microflora which can act as an opportunistic pathogen leading to various
Niloofar Khodaei   +7 more
doaj   +1 more source

Screening of highly efficient photosynthetic hybrids of Oryza officinalis and analysis of their photosynthetic pathway genes

open access: yesPhotosynthetica, 2021
Hereditary properties of strong growth and huge accumulation of biomass in Oryza officinalis exhibit a great potential; however, the genes that code for its high photosynthesis performance are not established.
E.X. LI   +7 more
doaj   +1 more source

Comparison of methods to extract PCR-amplifiable DNA from fruit, herbal and black teas

open access: yesCzech Journal of Food Sciences, 2021
The success of polymerase chain reaction (PCR) assay depends on template deoxyribonucleic acid (DNA) being sufficient with respect to both quantity and quality.
Eliška Čermáková   +3 more
doaj   +1 more source

Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology

open access: yesGuangdong nongye kexue, 2023
【Objective】The study aims to explore the composition, sequence number and diversity of microbial community in Zhuhai Wailingding Marine Ranch, analyze the sensitivity and reducing power of eDNA technology, with a view to providing references for the ...
Nengwei ZANG   +5 more
doaj   +1 more source

Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia

open access: yesJurnal Ilmiah Perikanan dan Kelautan, 2023
Highlight Research • The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples.
Hatika Hairani   +6 more
doaj   +1 more source

Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata)

open access: yesJurnal Riset Biologi dan Aplikasinya, 2021
Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences,
Chumaidatul Choiriyah, S.Si   +4 more
doaj   +1 more source

Home - About - Disclaimer - Privacy