Bioinformatics and Biology InsightsThe polymerase chain reaction (PCR) amplification process of deoxyribonucleic acid (DNA) libraries can introduce bias in the sequence ratios. Consequently, several recent genomic and transcriptomic methods employing next-generation sequencing (NGS ...
Ryoga Suzuki +4 more
doaj +1 more source
A snapshot on molecular technologies for diagnosing FAdV infections
Frontiers in Veterinary ScienceFowl adenoviruses (FAdV) are prevalent in chickens worldwide, responsible for several poultry diseases, including inclusion body hepatitis (IBH), hepatitis-hydropericardium syndrome (HHS), and gizzard erosion (GE), which result in significant economic ...
Amina Kardoudi +6 more
doaj +1 more source
Excessive addition split peak formed by the non-templated nucleotide addition property of Taq DNA polymerase after PCR amplification. [PDF]
Front Bioeng Biotechnol, 2023 Zhou Y, Bo F, Tian T, Wu B, Zhu B.
europepmc +1 more source
Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin
Molecular Oncology, EarlyView.The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla +10 more
wiley +1 more source
Long-range PCR amplification-based targeted enrichment & next generation sequencing: A cost-effective testing strategy for lysosomal storage disorders. [PDF]
Indian J Med Res, 2023 Vanaja MC +3 more
europepmc +1 more source
Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity
Molecular Oncology, EarlyView.Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung +17 more
wiley +1 more source
SARS-CoV-2 variants of concern are associated with lower RT-PCR amplification cycles between January and March 2021 in France. [PDF]
Int J Infect Dis, 2021 Roquebert B +8 more
europepmc +2 more sources
Real-Time fast PCR amplification using designated and conventional real time thermal cycler systems: COVID-19 perspective. [PDF]
PLoS One, 2022 Hossain MW +7 more
europepmc +1 more source
Molecular Technique to Understand Deep Microbial Diversity [PDF]
Current sequencing-based and DNA microarray techniques to study microbial diversity are based on an initial PCR (polymerase chain reaction) amplification step.
Vaishampayan, Parag A. +1 more
core +1 more source
A multiplex quantitative real time PCR to detect Xanthomonas axonopodis pv.allii from onion seeds : [P2-26] [PDF]
, 2010 Bacterial blight of onion is a seed-borne emerging disease threatening world onion production, and causing damage to other Allium crops. Its causal agent, #Xanthomonas axonopodis pv.
Escalon, Aline +3 more
core