Results 131 to 140 of about 709,414 (333)

Efficient PCR Amplification Protocol of Nuclear Microsatellites for Exuviae-Derived DNA of Cicada, Yezoterpnosia nigricosta. [PDF]

Front Insect Sci, 2021
Yumoto K, Kanbe T, Saito Y, Kaneko S, Tsuda Y.   +4 more
europepmc   +1 more source

Experimental Analysis of XPCR-based protocols [PDF]

arXiv, 2017
This paper reports some experimental results validating in a broader context a variant of PCR, called XPCR, previously introduced and tested on relatively short synthetic DNA sequences. Basic XPCR technique confirmed to work as expected, to concatenate two genes of different lengths, while a library of all permutations of three different genes ...
arxiv  

Red blood cells could protect miRNAs from degradation or loss thanks to Argonaute 2 binding

FEBS Open Bio, EarlyView.
The present work investigates the presence and the stability of miRNAs into RBCs, both native and engineered. The observed stability is due to the Ago2 bound resulting in minimal RISC. Indeed, if the miRNA‐Ago2 complex is present, miRNAs are protected from release or degradation and they are biologically active. Thus, RBCs can act as miRNA carriers for
Elena Perla, Faiza Abbas, Luigia Rossi, Mauro Magnani, Sara Biagiotti   +4 more
wiley   +1 more source

DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting [PDF]

, 1994
Igor Brikun, Kęstutis Sužiedėlis, Douglas E. Berg   +2 more
openalex   +1 more source

Characterisation of the role played by ELMO1, GPR141 and the intergenic polymorphism rs918980 in Fuchs' dystrophy in the Indian population

FEBS Open Bio, EarlyView.
This study reports the upregulation of ELMO1 and GPR141 in human Fuchs' endothelial corneal dystrophy (FECD) corneal endothelium and ultraviolet A‐induced FECD mice model. A genetic association of an intergenic single nucleotide polymorphism rs918980 present between both genes is observed with FECD in the Indian population.
Susmita Sharma, Samar Kumar Basak, Sujata Das, Debasmita Pankaj Alone   +3 more
wiley   +1 more source

Mutagenic oligonucleotide-directed PCR amplification (Mod-PCR): an efficient method for generating random base substitution mutations in a DNA sequence element. [PDF]

, 1993
Lillian W. Chiang, Iulia A. Kovari, Martha M. Howe   +2 more
openalex   +1 more source

Prediction of PCR amplification from primer and template sequences using recurrent neural network. [PDF]

Sci Rep, 2021
Kayama K, Kanno M, Chisaki N, Tanaka M, Yao R, Hanazono K, Camer GA, Endoh D.   +7 more
europepmc   +1 more source

Mapping Hsp104 interactions using cross‐linking mass spectrometry

FEBS Open Bio, EarlyView.
This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal, Karolina Michalska, Andrzej Joachimiak, Lukasz A. Joachimiak   +3 more
wiley   +1 more source

A DNA extraction method that allows reliable PCR amplification of MLO DNA from "difficult" plant host species. [PDF]

, 1994
Karen Gibb, Anna Padovan
openalex   +1 more source

A Novel Method to Construct Binary CRISPR Vectors for Plant Transformation by Single Round of PCR Amplification. [PDF]

Bio Protoc, 2021
Li K, Wang Y, Fang C.
europepmc   +1 more source