Results 141 to 150 of about 709,414 (333)

Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria

FEBS Open Bio, EarlyView.
The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao, Takato Ozaki, Hirofumi Fukuda, Yu Kanesaki, Munehiko Asayama   +4 more
wiley   +1 more source

DETECTION OF EPIDERMAL GROWTH-FACTOR RECEPTOR AND C-ERBB-2 GENE AMPLIFICATION IN TRANSITIONAL-CELL BLADDER-CARCINOMA USING THE DIFFERENTIAL PCR TECHNIQUE [PDF]

, 1994
Vassilis G. Gorgoulis, Christos Tsatsanis, Brad Ozanne, Demetrios�� Spandidos   +3 more
openalex   +1 more source

Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS‐PAGE with low acrylamide concentration

FEBS Open Bio, EarlyView.
SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel, Qian He, Yixin Kang, Dianbao Zhang, Min Gao, Minglin Wang, Kexin Fan, Jingwen Xiong, Shaobo Wu, Botao Fa, Zhengtao Xiao, Yingfang Niu, Jun Yao, Yilei Zhang   +13 more
wiley   +1 more source

Interspersed repetitive sequence (IRS)-PCR amplification of pulsed-field gel fractionated DNA to derive markers from the incontinentia pigmenti 1 (IP1) locus [PDF]

, 1993
Eric N. Burright, Jerome L. Gorski
openalex   +1 more source

Direct PCR amplification from saliva sample using non-direct multiplex STR kits for forensic DNA typing. [PDF]

Sci Rep, 2021
Shrivastava P, Jain T, Kumawat RK.
europepmc   +1 more source

On subcellular distribution of the zinc finger 469 protein (ZNF469) and observed discrepancy in the localization of endogenous and overexpressed ZNF469

FEBS Open Bio, EarlyView.
ZNF469 regulates the expression of genes encoding extracellular matrix proteins. Endogenous ZNF469 is predominantly cytoplasmic, while in transfected cells, it forms aggregates reminiscent of biomolecular condensates, located mainly in the nucleus. These condensates exhibit overlapping staining with proteasomes and are also associated with the mitotic ...
Anne Elisabeth Christensen Mellgren, Ileana Cristea, Thomas Stevenson, Endy Spriet, Per Morten Knappskog, Stig Ove Bøe, Harald Kranz, Sushma N. Grellscheid, Eyvind Rødahl   +8 more
wiley   +1 more source

Genetic Fingerprinting of <i>Bacillus thuringiensis</i> Isolates by Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)

, 1970
Gyan S. Sahukhal, Upendra Thapa Shrestha, Binod Lekhak   +2 more
openalex   +2 more sources

Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications [PDF]

, 1992
Quin Chou, Marion Russell, David Birch, Jonathan Raymond, Will Bloch   +4 more
openalex   +1 more source

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio, EarlyView.
ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
wiley   +1 more source

Direct amplification of cDNA inserts from λ libraries using the cloning-adapter as primer for PCR

, 1989
Ulla Rasmussen, Paul Basset, Jean‐Yves Daniel   +2 more
openalex   +2 more sources