Results 11 to 20 of about 1,169,901 (341)
Fast Thermocycling in Custom Microfluidic Cartridge for Rapid Single-Molecule Droplet PCR
Sensors, 2023 The microfluidic droplet polymerase chain reaction (PCR), which enables simultaneous DNA amplification in numerous droplets, has led to the discovery of various applications that were previously deemed unattainable.
Hirokazu Takahara +2 more
doaj +1 more source
Inside the Black Box: What Makes SELEX Better?
Molecules, 2019 Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications.
Natalia Komarova, Alexander Kuznetsov
doaj +1 more source
Microfluidic DNA amplification - a review [PDF]
, 2009 The application of microfluidic devices for DNA amplification has recently been extensively studied. Here, we review the important development of microfluidic polymerase chain reaction (PCR) devices and discuss the underlying physical principles for the ...
Ozdemir, Pinar, Zhang, Yonghao
core +1 more source
Robust CTAB-activated charcoal protocol for plant DNA extraction
Acta Agriculturae Slovenica, 2006 DNA extracted from plants rich in polyphenols and/or polysaccharides is often problematic when subjected to polymerase chain reaction, especially when mature tissues are used for DNA extraction.
Mitja KRIŽMAN +4 more
doaj +1 more source
An improved method for extraction of microbial DNA from alkaline-saline soil
Terra Latinoamericana, 2021 A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández +9 more
doaj +1 more source
Point of Care Diagnosis of Multiple Schistosome Parasites: Species-specific DNA Detection in Urine by Loop-mediated Isothermal Amplification (LAMP) [PDF]
, 2017 Schistosomes are easily transmitted and high chance of repeat infection, so if control strategies based on targeted mass drug administration (MDA) are to succeed it is essential to have a test that is sensitive, accurate and simple to use.
Anyan, William K. +6 more
core +2 more sources
Improved PCR Amplification of Broad Spectrum GC DNA Templates. [PDF]
PLoS ONE, 2016 Many applications in molecular biology can benefit from improved PCR amplification of DNA segments containing a wide range of GC content. Conventional PCR amplification of DNA sequences with regions of GC less than 30%, or higher than 70%, is complex due
Nicholas Guido +3 more
doaj +1 more source
Target enrichment using parallel nanoliter quantitative PCR amplification [PDF]
, 2014 Background: Next generation targeted resequencing is replacing Sanger sequencing at high pace in routine genetic diagnosis. The need for well validated, high quality enrichment platforms to complement the bench-top next generation sequencing devices is ...
De Wilde, Bram +7 more
core +2 more sources
Random Mutagenesis by Whole-Plasmid PCR Amplification
BioTechniques, 1998 Random mutagenesis has become a powerful means of studying the effects of a large number of permutations of a particular DNA sequence. As a prime example, libraries of randomized cDNA clones, when translated into their corresponding proteins, can be ...
Asit Parikh, F. Peter Guengerich
doaj +1 more source
An evaluation of direct PCR amplification [PDF]
Croatian Medical Journal, 2014 To generate complete DNA profiles from blood and saliva samples deposited on FTA® and non-FTA® paper substrates following a direct amplification protocol.Saliva samples from living donors and blood samples from deceased individuals were deposited on ten different FTA® and non-FTA® substrates.
Hall, Daniel E., Roy, Reena
openaire +2 more sources