Results 11 to 20 of about 709,414 (333)

Cross-Genera PCR Amplification of DNA from Apicomplexan Parasites [PDF]

open access: yesJournal of Arthropod-Borne Diseases, 2018
Background: The discovery of an unexpected genetic sequence raised doubts about the specificity of a primer pair targeting Babesia spp. and Theileria spp. This study aimed to check the specificity of this primer pair.
Philippe Gil de Mendonça
doaj   +2 more sources

Research on Bacteria Diversity of Marine Ranch in Zhuhai Wailingding Based on eDNA Technology

open access: yesGuangdong nongye kexue, 2023
【Objective】The study aims to explore the composition, sequence number and diversity of microbial community in Zhuhai Wailingding Marine Ranch, analyze the sensitivity and reducing power of eDNA technology, with a view to providing references for the ...
Nengwei ZANG   +5 more
doaj   +1 more source

Optimization of Various ITS rDNA Amplification Protocol of Yeast Isolated from Giant Honey Beehives (Apis dorsata)

open access: yesJurnal Riset Biologi dan Aplikasinya, 2021
Indonesia is a country with high variability of microorganisms, including bacteria, yeast, and fungi. Yeast isolates could be isolated from the honeycomb of Apis dorsata. Molecular approaches were used to identify yeast using ribosomal DNA gene sequences,
Chumaidatul Choiriyah, S.Si   +4 more
doaj   +1 more source

Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia

open access: yesJurnal Ilmiah Perikanan dan Kelautan, 2023
Highlight Research • The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples.
Hatika Hairani   +6 more
doaj   +1 more source

An improved method for extraction of microbial DNA from alkaline-saline soil

open access: yesTerra Latinoamericana, 2021
A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (
Valentín Pérez-Hernández   +9 more
doaj   +1 more source

Inside the Black Box: What Makes SELEX Better?

open access: yesMolecules, 2019
Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications.
Natalia Komarova, Alexander Kuznetsov
doaj   +1 more source

PCR Based Molecular Detection of the Gyr-B-2 Gene from the Klebsiella Sp. Isolates from Patients who were Suffering with Pneumonia and Urinary Tract Infections (UTIs) [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2013
Purpose: Detection of the virulence gene is a key component in determining the pathogenicity of any isolates, because these genes act multi-functionally and multi-factorially.
Md. Javed Foysal   +2 more
doaj   +1 more source

Ultrasensitive THz Biosensor for PCR-free cDNA detection based on frequency selective surfaces [PDF]

open access: yesBiomedical Optics Express, vol. 11, no. 1, 2020, p. 448, 2020
THz technologies are a powerful tool for label-free detection of biomolecules. However, significant reduction of the lower detection limit is required to apply THz-sensors in biomedical diagnosis. This paper reports an ultrasensitive THz-biosensor based on asymmetric double split ring resonators (aDSRR) for the direct label- and PCR-free detection of ...
arxiv   +1 more source

Fast Thermocycling in Custom Microfluidic Cartridge for Rapid Single-Molecule Droplet PCR

open access: yesSensors, 2023
The microfluidic droplet polymerase chain reaction (PCR), which enables simultaneous DNA amplification in numerous droplets, has led to the discovery of various applications that were previously deemed unattainable.
Hirokazu Takahara   +2 more
doaj   +1 more source

Robust CTAB-activated charcoal protocol for plant DNA extraction

open access: yesActa Agriculturae Slovenica, 2006
DNA extracted from plants rich in polyphenols and/or polysaccharides is often problematic when subjected to polymerase chain reaction, especially when mature tissues are used for DNA extraction.
Mitja KRIŽMAN   +4 more
doaj   +1 more source

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