PCR Based Molecular Detection of the Gyr-B-2 Gene from the Klebsiella Sp. Isolates from Patients who were Suffering with Pneumonia and Urinary Tract Infections (UTIs) [PDF]
Journal of Clinical and Diagnostic Research, 2013 Purpose: Detection of the virulence gene is a key component in determining the pathogenicity of any isolates, because these genes act multi-functionally and multi-factorially.
Md. Javed Foysal +2 more
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Gene-specific monitoring of T7-based RNA amplification by real-time quantitative PCR
BioTechniques, 2003 T7-based RNA amplification is applied when there is insufficient RNA. The overall extent of amplification can be measured spectrophotometrically (i.e., quantifying RNA yields), but this measurement does not provide information about the RNA amplification
Sandra G. Heil +4 more
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An advanced and efficient asymmetric PCR method for microarray applications
Frontiers in Bioengineering and Biotechnology, 2022 The sensitivity of a PCR based biochip assay relies on the efficiency of PCR amplicons in binding to the microarray spots. The essential factor determining the sensitivity is the amount of single stranded (ss) amplicons available for biochip ...
Suresh Reddy Banda +7 more
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Following basal stem rot in young oil palm plantings [PDF]
, 2005 The PCR primer GanET has previously been shown to be suitable for the specific amplification of DNA from Ganoderma boninense. A DNA extraction and PCR method has been developed that allows for the amplification of the G.
CA Levesque +17 more
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Multiplex STR amplification sensitivity in a silicon microchip [PDF]
, 2018 The demand for solutions to perform forensic DNA profiling outside of centralized laboratories is increasing. We here demonstrate highly sensitive STR amplification using a silicon micro-PCR (mu PCR) chip.
Colle, Frederik +8 more
core +2 more sources
‘Next‐base’ effect on PCR amplification
Environmental Microbiology Reports, 2011 Summary The base adjacent to the 3′ end of universal PCR primers targeting the 16S rRNA gene is often variable and apparently biases the microbial community composition as represented by PCR‐based surveys. To test this hypothesis, four templates of 44 bases each and two complementary primers (21 bases) were designed to differ only in ...
Ben-Dov, Eitan +2 more
openaire +4 more sources
Comparative evaluation of linear and exponential amplification techniques for expression profiling at the single-cell level [PDF]
, 2011 Background: Single-cell microarray expression profiling requires 10(8)-10(9)-fold amplification of the picogram amounts of total RNA typically found in eukaryotic cells.
Livesey, FJ, Subkhankulova, T
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Mutations and amplification of oncogenes in endometrial cancer [PDF]
, 1999 Alterations in oncogenes are critical steps in the development of endometrial cancer. To investigate the potential clinical relevance of the amplification of the oncogenes c-erbB2, c-myc, and int-2 and the mutation of K-ras in endometrial cancer, 112 ...
An, Han-Xiang +5 more
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The prognostic impact of EGFR, ErbB2 and MET gene amplification in human gastric carcinomas as measured by quantitative Real-Time PCR [PDF]
, 2015 Purpose: Identification of critical genes which play pivotal roles in controlling tumor growth and survival will establish the basis for developing therapeutic targets. In this study, we focused on frequencies of EGFR, ErbB2 and MET gene amplification in
Farazmandfar, T. +4 more
core +1 more source
BioTechniques, 1998 Previously we described a PCR protocol for detecting the inversion in the factor VIII gene, which is a common cause of Hemophilia A. This PCR assay is challenging due to the size of the amplification (10–12 kb), the varying GC content (30%–80%) and the ...
Qiang Liu, Steve S. Sommer
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