Results 21 to 30 of about 709,414 (333)
Random Mutagenesis by Whole-Plasmid PCR Amplification
BioTechniques, 1998 Random mutagenesis has become a powerful means of studying the effects of a large number of permutations of a particular DNA sequence. As a prime example, libraries of randomized cDNA clones, when translated into their corresponding proteins, can be ...
Asit Parikh, F. Peter Guengerich
doaj +1 more source
An advanced and efficient asymmetric PCR method for microarray applications
Frontiers in Bioengineering and Biotechnology, 2022 The sensitivity of a PCR based biochip assay relies on the efficiency of PCR amplicons in binding to the microarray spots. The essential factor determining the sensitivity is the amount of single stranded (ss) amplicons available for biochip ...
Suresh Reddy Banda +7 more
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Improved PCR Amplification of Broad Spectrum GC DNA Templates. [PDF]
PLoS ONE, 2016 Many applications in molecular biology can benefit from improved PCR amplification of DNA segments containing a wide range of GC content. Conventional PCR amplification of DNA sequences with regions of GC less than 30%, or higher than 70%, is complex due
Nicholas Guido +3 more
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Optimized protocol for DNA extraction from ancient skeletal remains using Chelex-100 [PDF]
, 2021 PCR-based analysis of skeletonized human remains is a common aspect in both forensic human identification as well as Ancient DNA research. In this, both areas not merely utilize very similar methodology, but also share the same problems regarding quantity and quality of recovered DNA and presence of inhibitory substances in samples from excavated ...
arxiv +1 more source
MegaPlex PCR: a strategy for multiplex amplification [PDF]
Nature Methods, 2007 'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair.
Meuzelaar, Linda Strömqvist +4 more
openaire +3 more sources
Current and perspective sensing methods for monkeypox virus: a reemerging zoonosis in its infancy [PDF]
arXiv, 2022 Objectives The review is dedicated to evaluate the current monkeypox virus (MPXV) detection methods, discuss their pros and cons, and provide recommended solutions to the problems. Methods The literature for this review is identified through searches in PubMed, Web of Science, Google Scholar, ResearchGate, and Science Direct advanced search for ...
arxiv
Dimethyl sulfoxide improves sensitivity and specificity of RT-PCR and qRT-PCR amplification of low-expressed transgenes [PDF]
Archives of Biological Sciences, 2012 The expression of transgenes in a host plant may be low for a number of reasons. Both low expression and poor specificity of amplification were encountered during analysis of the expression of the Arabidopsis cytokinin oxidase/dehydrogenase (AtCKX1 ...
Trifunović Milana +6 more
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Cloning, expression, purification and characterization of Leishmania tropica PDI-2 protein
Open Life Sciences, 2016 In Leishmania species, protein disulfide isomerase (PDI) is an essential enzyme that catalyzes thiol-disulfide interchange. The present work describes the isolation, cloning, sequencing and expression of the pdI-2 gene.
Ali Dina +3 more
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Gene-specific monitoring of T7-based RNA amplification by real-time quantitative PCR
BioTechniques, 2003 T7-based RNA amplification is applied when there is insufficient RNA. The overall extent of amplification can be measured spectrophotometrically (i.e., quantifying RNA yields), but this measurement does not provide information about the RNA amplification
Sandra G. Heil +4 more
doaj +1 more source
Detection of short DNA sequences with DNA nanopores [PDF]
arXiv, 2021 Several studies suggest strong correlation between different types of cancer and the relative concentration of short circulating RNA sequences (miRNA). Because of short length and low concentration, miRNA detection is not easy. Standard methods such as RT-PCR require both the standard PCR amplification step and a preliminary additional step of reverse ...
arxiv