Results 301 to 310 of about 1,169,901 (341)

MYCN Amplification by Differential PCR

Pediatric Hematology and Oncology, 1993
A method is described to estimate MYCN (N-myc) oncogene amplification in neuroblastoma by the technique of differential polymerase chain reaction (PCR). The technique is quicker than conventional Southern blotting techniques and does not require radioactive materials.
S N, Huddart, J R, Mann, A G, McGukin, R, Corbett   +3 more
openaire   +2 more sources

PCR-amplification of D2S44 alleles

International Journal of Legal Medicine, 1994
A strategy for PCR-amplification and sequencing of the flanking regions in the polymorphism D2S44 (YNH24) has been developed based on the investigations of Edwards et al. (1991). The flanking regions of the YNH24 probe were successfully amplified and two distinct PCR products with fragment sizes of 180 and 250 bp obtained.
A, Möller, C, Puers, B, Brinkmann
openaire   +2 more sources

PCR-amplification of GC-rich regions: 'slowdown PCR'

Nature Protocols, 2008
The polymerase chain reaction (PCR) technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures.
Ulrich H, Frey, Hagen S, Bachmann, Jürgen, Peters, Winfried, Siffert   +3 more
openaire   +2 more sources

Gene Amplification and qRT-PCR

2014
This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell
Cerith, Jones, Alain, Filloux
openaire   +2 more sources

Applied Biosystems’ GlobalFiler™ PCR Amplification Kit

2023
The GlobalFiler™ PCR Amplification Kit is one of the most sensitive kits that exist today that makes the PCR amplification of human DNA possible. PCR amplification using this specific kit makes millions of copies of 24 specific target sequences in the DNA, called markers or loci.
Georgia Williams, Megan M. Foley, Kelly L. Knight   +2 more
openaire   +2 more sources

PCR microfluidic devices for DNA amplification

Biotechnology Advances, 2006
The miniaturization of biological and chemical analytical devices by micro-electro-mechanical-systems (MEMS) technology has posed a vital influence on such fields as medical diagnostics, microbial detection and other bio-analysis. Among many miniaturized analytical devices, the polymerase chain reaction (PCR) microchip/microdevices are studied ...
Chunsun, Zhang, Jinliang, Xu, Wenli, Ma, Wenling, Zheng   +3 more
openaire   +2 more sources

Enzymatic Amplification of RNA by PCR (RT‐PCR)

Current Protocols in Molecular Biology, 2001
AbstractMethods for enzymatic amplification of RNA by the polymerase chain reaction (RT‐PCR) are highlighted in this unit. The is especially useful for rare RNAs because all steps (annealing, reverse transcription, and amplification) are performed under optimal conditions, thereby maximizing efficiency and recovery.
openaire   +2 more sources

PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification

2015
The field of molecular biology was revolutionized with the development of the polymerase chain reaction (PCR). This chapter defines PCR, reverse transcription PCR (RT-PCR), real-time PCR, digital PCR and isothermal amplification. Within each subject a brief overview of the process is given along with the required reagents or components and highlighted ...
Rachel A. Bartholomew, Janine R. Hutchison, Timothy M. Straub, Douglas R. Call   +3 more
openaire   +1 more source

PCR Amplification of Base‐Modified DNA

Current Protocols in Chemical Biology, 2018
AbstractAn efficient PCR amplification of various templates (short 57‐mer, random 67‐ and 82‐mer, and long DNA) with base‐modified nucleoside triphosphates is presented here. Using 5‐substituted pyrimidine and 7‐substituted‐7‐deaza‐ or 8‐substituted purine nucleoside triphosphates as substrates for thermostable DNA polymerases [Taq and Vent (exo ...
Eremeeva, Elena, Herdewijn, Piet
openaire   +3 more sources

Non-PCR Amplification Techniques

2018
Signal, probe, and non-PCR target amplification methods have been developed as viable alternatives to PCR testing for infectious agents. These signal amplification methods include hybrid capture and Invader, as well as target amplification methods such as transcription-mediated amplification, strand displacement amplification, loop-mediated isothermal ...
Rosemary C. She, Ted E. Schutzbank, Elizabeth M. Marlowe   +2 more
openaire   +1 more source