Results 311 to 320 of about 1,169,901 (341)

Multiplex PCR Amplification of Ancient DNA

2011
Multiplex PCR allows the simultaneous amplification of up to dozens of target fragments in a single PCR. It is therefore a powerful tool to obtain many kilobases of continuous sequence from minute amounts of ancient DNA (aDNA), which usually must be amplified in multiple short and overlapping fragments.
Mathias, Stiller, Tara L, Fulton
openaire   +2 more sources

PCR Amplification of DNA

Journal of Chemical Education, 1994
A polymerase chain reaction (PCR) student exercise using a teacup thermocycler and a commercially available thermocycler.
openaire   +1 more source

PCR-mediated whole genome amplification of phytoplasmas

Journal of Microbiological Methods, 2004
A method was developed for genome analysis of phytoplasmas, bacterial plant pathogens that cannot be cultivated in vitro in cell-free media. The procedure includes a CsCl-bisbenzimide gradient buoyant centrifugation followed by polymerase chain reaction (PCR)-mediated whole genome amplification.
GARCIA CHAPA M., A. BATLLE, REKAB, Djaouida, RUIZ ROSQUETE M., FIRRAO, Giuseppe   +4 more
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Touchdown PCR for increased specificity and sensitivity in PCR amplification

Nature Protocols, 2008
Touchdown (TD) PCR offers a simple and rapid means to optimize PCRs, increasing specificity, sensitivity and yield, without the need for lengthy optimizations and/or the redesigning of primers. TD-PCR employs an initial annealing temperature above the projected melting temperature (T(m)) of the primers being used, then progressively transitions to a ...
Korbie, D. J., Mattick, J. S.
openaire   +3 more sources

PCR amplification reactions in parasitology

Journal of Microbiological Methods, 1995
The development of PCR systems for the detection and identification of pathogenic human parasites has only recently started. Evaluation of most of these methods is still lacking. Most likely these methods will need considerable improvement before reliable diagnostic methods become available.
Henk L. Smits, Rudy A. Hartskeerl
openaire   +1 more source

PCR amplification of antibody genes

Methods, 1991
A general method for directly obtaining the DNA sequence of the variable regions of any immunoglobulin chain by using a mixture of oligomer primers and the polymerase chain reaction (PCR) is described. Mixed oligonucleotide primers corresponding to the 5′ signal peptide or framework 1 and a conserved 3′ constant region primer were used for enzymatic ...
James W. Larrick, Kirk E. Fry
openaire   +1 more source

Quintuplex PCR-amplification of microsatellites.

Nihon hoigaku zasshi = The Japanese journal of legal medicine, 1996
We tested the potential usefulness of quintuplex PCR-amplification of STRs (D16S537, D8S320, FGA, D11S554 and THO1) for paternity testing, first by constructing 20 false family trios, each with a non-biological father, to estimate the actual exclusion rate, and next by a retrospective study of 20 paternity cases which had been previously tested by RFLP
W, Wang, M, Fukuda, T, Kishida, Y, Tamaki   +3 more
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Amplification of Genomic DNA by PCR

2003
The polymerase chain reaction (PCR) is used to amplify a segment of DNA that lies between two regions of known sequence (1-3). It requires two oligonucleotide primers that flank the DNA fragment to be amplified and employs repeated cycles of heat denaturation of the DNA, annealing of the primers to their complementary sequences, and extension of the ...
openaire   +2 more sources

Non-PCR Target Amplification Techniques

2012
In 1983 while driving up a mountain road, Dr Kary Mullis envisioned the concept of the polymerase chain reaction (PCR). These scientific “driving” thoughts completely revolutionized biology and bolstered an entire biotechnology industry, resulting in the creation of new biotechnology companies and jobs.
Rosemary C. She, Elizabeth M. Marlowe
openaire   +1 more source

The Limit of PCR Amplification

Journal of Theoretical Biology, 1996
D S, Dimitrov, M A, Apostolova
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