Results 61 to 70 of about 30,761 (276)

Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]

open access: yes, 2019
Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan   +4 more
core   +1 more source

Directed Functionalization of Recombinant Spider Silk Nonwoven Membranes with Antibodies Using Non‐Canonical Amino Acids

open access: yesAdvanced Materials, EarlyView.
A recombinant spider silk protein was functionalized by incorporating genetically encoded bio‐orthogonal reactive azido‐groups. The azido‐functionalized proteins were produced in a scalable biotechnological process, and particles, films, as well as nanofibers were established as anchorage for fully functional antibodies via site‐specific bio‐orthogonal
Claudia Lacombe   +4 more
wiley   +1 more source

Penggunaan Osmotikum Dalam Menunda Kerusakan Benih Kakao (Theobroma Cacao. L) [PDF]

open access: yes, 2014
Cacao seeds are recalcitrant seeds quickly lose its growing when not immediately dikecambahkan after the last of the parent plant. Cocoa seeds can not be dried below the critical water and not tolerant of low temperatures.
Nengsih, Y. (Yulistiati)
core  

Mitochondria‐Specific Protein Delivery by Protease‐Triggered Release in Plants with Single‐Walled Carbon Nanotubes

open access: yesAdvanced Materials Technologies, EarlyView.
Polymer‐coated carbon nanotubes were engineered as protease‐responsive nanocarriers for protein delivery in intact plants. GFP cargo can be released by cytosolic phytaspase cleavage and subsequently targeted to mitochondria via an N‐terminal sequence, enabling controlled intracellular protein delivery without tissue damage and demonstrating stable ...
Simon Sau Yin Law   +3 more
wiley   +1 more source

Physiological conditioning of pearl millet seeds [PDF]

open access: yes
A pesquisa foi realizada com o objetivo de avaliar o condicionamento fisiológico de sementes de três lotes de milheto do cultivar BRS 1501. Os tratamentos consistiram da testemunha, sementes hidratadas em água pura (potencial zero) e em oito potenciais ...
NOVEMBRE, Ana Dionisia da Luz Coelho   +1 more
core   +1 more source

Crystallization and preliminary crystallographic analysis of the DNA gyrase B protein from B-stearothermophilus [PDF]

open access: yes, 1996
DNA gyrase B (GyrB) from B. stearothermophilus has been crystallized in the presence of the non-hydrolyzable ATP analogue, 5'-adenylpl-beta-gamma-imidodiphosphate (ADPNP), by the dialysis method.
Brannigan, J A   +4 more
core   +2 more sources

Dual‐Functional Ingestible Passive Capsules for High‐Throughput Intestinal Sampling with Sealed Containment and Targeted Drug Delivery

open access: yesAdvanced Robotics Research, EarlyView.
To address challenges in high‐throughput intestinal sampling with sealed containment and target drug delivery, we developed a dual‐functional ingestible passive capsule with a dual‐triggered control system based on pH‐response and mechanical actuation.
Libing Huang   +9 more
wiley   +1 more source

Germinação de sementes de Caesalpinia pyramidalis Tul. (Catingueira) submetidas a deficiência hídrica

open access: yesRevista Árvore, 2011
A disponibilidade hídrica é um dos principais fatores ambientais capaz de influenciar o processo de germinação de sementes. Nessas condições, a habilidade das sementes em manter sua viabilidade durante o armazenamento pode ser uma vantagem adaptativa de ...
Cimille Gabrielle Cardoso Antunes   +5 more
doaj   +1 more source

RESPON MORFOLOGIS BEBERAPA GENOTIPE DAN VARIETAS PADI (ORYZA SATIVA L.) AKIBAT KEKERINGAN DENGAN KONSENTRASI PEG 6000 PADA FASE PERKECAMBAHAN [PDF]

open access: yes, 2016
RINGKASANPenelitian ini bertujuan untuk mengetahui respon morfologis beberapa genotip dan varietas padi pada kondisi kekeringan oleh PEG (Polyethillen Glicol) 6000 sebagai indikator ketahanan genotipe dan varietas terhadap kekeringan, serta interaksi ...
Muzakir
core  

Disengaging the Engine: Histone Deacetylases 1 and 2‐Mediated Acetylation of Hexokinase‐2 Regulates Energy Metabolism in Microglia Following Intracerebral Hemorrhage

open access: yesAdvanced Science, EarlyView.
This study demonstrates that HDAC1/2 knockout in microglia alleviates neurological deficits, preserves white matter, and accelerates hematoma clearance after ICH. HDAC1/2 inhibition reduces HK2 acetylation, shifts metabolism from glycolysis to fatty acid oxidation, reduces inflammation, and enhances phagocytosis.
Zhiwen Jiang   +9 more
wiley   +1 more source

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