Results 271 to 280 of about 362,157 (307)

Isolation and characterization of a peptide chain initiation factor from Krebs II ascites tumor cells

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
Abstract A protein factor promoting the binding of fMet-tRNA Met f to 40-S ribosomal subunits or to 80-S ribosomes was isolated from the 0.5 M KCl ribosomal wash of Krebs II ascites tumor cells. By means of hydroxyapatite column chromatography, this factor could be separated from another accompanying protein which bound fMet-tRNA Met f ...
Eich, F, Drews, J
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Peptide Chain Elongation: Discrimination against the Initiator Transfer RNA by Microbial Amino-acid Polymerization Factors

Nature, 1968
The amino-acid polymerization factors S1 and S3 form complexes with most aminoacyl-tRNA species, including met-tRNAM, but not F-met-tRNAF and not met-tRNAF. This discrimination may be a basis for avoiding one kind of ambiguity in translation.
Y, Ono, A, Skoultchi, A, Klein, P, Lengyel   +3 more
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Protein synthesis in rabbit reticulocytes: Control of protein synthesis initiation by a Met-tRNAfMet deacylase and peptide chain initiation factors

Biochemical and Biophysical Research Communications, 1973
Abstract The 0.5M KCl wash of rabbit reticulocyte ribosomes (I fraction) catalyzes the deacylation of Met-tRNA f Met . Upon DEAE-cellulose column chromatography, the deacylase activity elutes with the 0.1M KCl wash of the column (f1) and is well-resolved from the peptide chain initiation factors (1–3). The deacylase activity is specific for Met-tRNA
N K, Gupta, R J, Aerni
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Phosphorylation of only serine-51 in protein synthesis initiation factor-2 is associated with inhibition of peptide-chain initiation in reticulocyte lysates

Biochemical and Biophysical Research Communications, 1991
We have examined the phosphorylation of the alpha-subunit of initiation factor-2 (eIF-2 alpha) in reticulocyte lysates in which translational shut-off was induced by haem-deficiency or by double-stranded RNA. To maximise the phosphorylation of eIF-2 alpha, lysates were supplemented with the broad spectrum phosphatase inhibitor microcystin.
N T, Price, G I, Welsh, C G, Proud
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Inhibiting the Interaction Between von Willebrand Factor and Collagen Using Novel Peptides to Prevent Thrombosis Initiation

The FASEB Journal, 2022
The field of peptide pharmaceuticals is gaining important prominence. Peptides have an attractive pharmacological profile and excellent safety, tolerability and efficacy in humans which makes them ideal candidates for new drugs. The macrocyclic arrangements of the amino acids in peptides can provide added stability, therefore the use of cyclic or ...
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Control of Protein Synthesis. Phosphorylation and Dephosphorylation of Eukaryotic Peptide Initiation Factor 2

1981
Differentiation, virus infection, or certain pathological conditions are examples of physiologic events that cause dramatic qualitative and quantitative changes in protein synthesis. It has been assumed and directly shown in several model systems that these changes involve a block in peptide initiation. Systems in which some of the underlying molecular
G. Kramer, N. Grankowski, B. Hardesty
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In Vitro Growth-Promoting Activity of Porcine Mammary Secretions: Initial Characterization and Relationship to Known Peptide Growth Factors

Journal of Animal Science, 1987
We have examined the in vitro growth-promoting properties and growth factor content of porcine mammary secretions. Defatted, porcine colostrum stimulated the proliferation of fibroblast and epithelial-like cell lines of diverse species origins in serum-free medium and cellular DNA synthesis (4- to 119-fold) as monitored by uptake of 3H-thymidine into ...
K, Cera, D C, Mahan, F A, Simmen
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In vitro synthesis of collagen peptides on fetal and neonatal rat skin polysomes by rabbit reticulocyte initiation factors

Archives of Biochemistry and Biophysics, 1974
Abstract Salt-washed polysomes isolated from fetal or neonatal rat skin were approximately 50% as active as rabbit reticulocyte salt-washed polysomes (per A 260 unit) in the incorporation of [ 14 C]leucine in a protein-synthesizing system derived entirely from rabbit reticulocytes.
P M, Prichard, G W, Staton, K R, Cutroneo   +2 more
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A Rationally Designed Antimicrobial Peptide from Structural and Functional Insight ofClostridium difficileTranslation Initiation Factor 1

2023
ABSTRACTA significant increase of hospital-acquired bacterial infections during the COVID-19 pandemic has become an urgent medical problem.Clostridioides difficileis an urgent antibiotic-resistant bacterial pathogen and a leading causative agent of nosocomial infections. The increasing recurrence ofC.
Elvira Alanis, Faith Aguilar, Niaz Banaei, Frank B. Dean, Miguel Alanis, James M. Bullard, Yonghong Zhang   +6 more
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Synthetic peptide probe folding initiation sites in platelet factor-4: stable chain reversal found within the hydrophobic sequence LIATLKNGRKISL

Biochemistry, 1994
Platelet factor-4 (PF4) is a 70-residue protein which contains a 3-stranded antiparallel beta-sheet domain on to which is folded a C-terminal alpha-helix and an aperiodic N-terminal region. In this study, three peptides derived from the beta-sheet (residues 24-46 and 38-57) and helix (residues 57-70) domains have been synthesized and studied in aqueous
E, Ilyina, R, Milius, K H, Mayo
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