Results 41 to 50 of about 910,956 (264)
Performing SELEX experiments in silico
The Journal of Chemical Physics, 2017 Due to the sequence-dependent nature of the elasticity of DNA, many protein-DNA complexes and other systems in which DNA molecules must be deformed have preferences for the type of DNA sequence they interact with. SELEX (Systematic Evolution of Ligands by EXponential enrichment) experiments and similar sequence selection experiments have been used ...
Wondergem, A.J. +2 more
openaire +4 more sources
Innovative Methods for Assessing Students’ Performance in the ESP Classroom [PDF]
Ovidius University Annals: Economic Sciences Series, 2018 Learning is usually assessed through performance, i.e. what students can do with the acquiredknowledge and skills. In its turn, assessment (formal or informal, high- or low-stakes, anonymousor public, individual or collective) is an integral part of the ...
Nădrag Lavinia +1 more
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FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Zhileng xuebao, 2018 To solve the problem of heating in electric vehicle air-conditioning systems and suppressing the high discharge temperature of the compressor in winter, a quasi-two-stage compression heat pump system for electric vehicles has been designed using flash ...
Tang Jingchun +3 more
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Case Studies in Construction MaterialsThe cementitious paste containing a high volume of calcined clay exhibits poor fluidity due to the thixotropic nature of the clay suspension. The study focuses on the impact of the stepwise addition of PCE and protein-based alkaline retarder (PAR) on the
Jun Ren +8 more
doaj +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Zhileng xuebao, 2015 Based on the climate characteristics of the Yangtze River in China and the disadvantages of radiation air conditioning, an energy-saving and temperature-controlled fresh air dehumidifier of radiation air conditioning was designed to remove fresh air load
Chen Jianbo +4 more
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