Results 171 to 180 of about 2,525 (205)

Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

Journal of Visualized Experiments, 2021
Mitochondrial substrate flux is a distinguishing characteristic of each cell type, and changes in its components such as transporters, channels, or enzymes are involved in the pathogenesis of several diseases. Mitochondrial substrate flux can be studied using intact cells, permeabilized cells, or isolated mitochondria.
Moustafa, Elkalaf, Karolína, Vaněčková, Pavla, Staňková, Zuzana, Červinková, Jan, Polák, Otto, Kučera   +5 more
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Age-development changes in susceptibility of erythrocytes to perfringolysin O

Mechanisms of Ageing and Development, 1982
Susceptibility to perfringolysin O of erythrocytes from mice of different ages was examined. Erythrocytes of mice younger than 5 weeks' old were more resistant to the toxin than those of young adult and adult mice. Erythrocytes of aged mice were about 3.5 times more susceptible to the toxin than erythrocytes from 4-week-old mice.
Saito, M, Ando, S, Tanaka, Y, Nagai, Y, Mitsui, K, Hase, J   +5 more
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Activation of phosphatidic acid metabolism of human erythrocyte membranes by perfringolysin O

Biochemical and Biophysical Research Communications, 1986
The effect of perfringolysin O on the lipid metabolism of human erythrocyte membranes was investigated. Erythrocytes were prelabeled with [3H]arachidonic acid and [32P]inorganic phosphate. In the presence of calcium ion(5.5 mM), the effect of perfringolysin O on lipid metabolism was very similar to that of an calcium-ionophore A23187. In the absence of
M, Saito, S, Ando, K, Mitsui, Y, Homma, T, Takenawa   +4 more
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Ring formation of perfringolysin o as revealed by negative stain electron microscopy

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1979
Abstract Perfringolysin O revealed ring- and arc-shaped structures in the absence of cholesterol by negative staining electron microscopy, while before activation with cysteine it showed indistinct arcs and irregularly curved sticks but no rings. These structures were observed only at high concentrations (more than 17 000 hemolytic units per ml) and ...
K, Mitsui, T, Sekiya, S, Okamura, Y, Nozawa, J, Hase   +4 more
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Effects of cholesterol evulsion on susceptibility to perfringolysin o of human erythrocytes

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1982
Human erythrocytes preincubated with a phosphatidylcholine suspension (preincubated cells) showed decreased susceptibility to perfringolysin O, the decrease being strongly affected by preincubation time and temperature, and the phosphatidyl choline concentration.
K, Mitsui, Y, Saeki, J, Hase
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Perfringolysin O, a cholesterol-binding cytolysin, as a probe for lipid rafts

Anaerobe, 2004
Gaining an understanding of the structural and functional roles of cholesterol in membrane lipid rafts is a critical issue in studies on cellular signaling and because of the possible involvement of lipid rafts in various diseases. We have focused on the potential of perfringolysin O (theta-toxin), a cholesterol-binding cytolysin produced by ...
Yoshiko, Ohno-Iwashita, Yukiko, Shimada, A Abdul, Waheed, Masami, Hayashi, Mitsushi, Inomata, Megumi, Nakamura, Mikako, Maruya, Shintaro, Iwashita   +7 more
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Helical crystallization on nickel–lipid nanotubes: Perfringolysin O as a model protein

Journal of Structural Biology, 2005
To facilitate purification and subsequent structural studies of recombinant proteins the most widely used genetically encoded tag is the histidine tag (His-tag) which specifically binds to N-nitrilotriacetic-acid-chelated nickel ions. Lipids derivatized with a nickel-chelating head group can be mixed with galactosylceramide glycolipids to prepare lipid
Thanh X, Dang, Ronald A, Milligan, Rodney K, Tweten, Elizabeth M, Wilson-Kubalek   +3 more
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Activation of the Calcium Permeability of Erythrocyte Membranes by Perfringolysin O

The Journal of Biochemistry, 1983
Calcium ions inhibited perfringolysin O-induced hemolysis at a concentration lower than 1 mM, but not the hemolysis by digitonin at 10 mM. The introduction of calcium ions into ghosts inhibited the lysis more strongly than the addition of calcium ions outside ghosts.
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Modifications in Perfringolysin O Domain 4 Alter the Cholesterol Concentration Threshold Required for Binding

Biochemistry, 2012
Changes in the cholesterol content of cell membranes affect many physiological and pathological events, including the formation of arterial plaques, the entry of virus into cells, and receptor organization. Measuring the trafficking and distribution of cholesterol is essential to understanding how cells regulate sterol levels in membranes ...
Johnson, Benjamin B., Moe, Paul C., Wang, David, Rossi, Kathleen, Trigatti, Bernardo L., Heuck, Alejandro P.   +5 more
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Quantitative Measurement of Cholesterol in Cell Populations Using Flow Cytometry and Fluorescent Perfringolysin O*

2017
Methods to quantify intracellular cholesterol are valuable for the study of its trafficking and storage in normal cells and in lysosomal storage disorders. Traditionally, cholesterol has been tracked using the small molecule, filipin. Filipin can be difficult to visualize and visualization can be cytotoxic as it requires UV illumination.
Jian, Li, Peter L, Lee, Suzanne R, Pfeffer   +2 more
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