Results 41 to 50 of about 645,807 (300)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Optimal medium use for continuous high density perfusion processes [PDF]

, 1992
Büntemeyer H, Wallerius C, Lehmann J. Optimal medium use for continuous high density perfusion processes. Cytotechnology. 1992;9(1-3):59-67.For maintenance of high cell density in continuous perfusion processes not only feeding with substrates but also ...
Lehmann, Jürgen, Büntemeyer, Heino, Wallerius, Claus   +2 more
core   +1 more source

Perfusion-weighted FREE maps using a FLASH sequence at 3T with a flip angle of 40°.

, 2022
A temporal perfusion-weighted MIP is showing the maximal amplitude of the entire heart cycle for each voxel (a) and the perfusion-weighted map (b) is presenting the maximum median amplitude within the parenchyma ROI.
Frank Wacker (281957), Agilo Luitger Kern (14135251), Marcel Gutberlet (456685), Mike P. Wattjes (6239945), Norman Kornemann (14135254), Nima Mahmoudi (6534023), Jens Vogel-Claussen (313462), Andreas Voskrebenzev (4669540), Filip Klimeš (9928454), Julian Glandorf (9928451)   +9 more
core   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Reference values and physiological characterization of a specific isolated pig kidney perfusion model [PDF]

, 2007
Background: Models of isolated and perfused kidneys are used to study the effects of drugs, hazardous or toxic substances on renal functions. Since physiological and morphological parameters of small laboratory animal kidneys are difficult to compare to ...
Unger Volker, David A Groneberg, Fischer, Axel, Unger, Volker, Groneberg, Jan David Alexander, Groneberg David A, Volker Unger, Grosse-Siestrup Christian, Fehrenberg Claudia, Meissler, Michael, Axel Fischer, Christian Grosse-Siestrup, Fischer Axel, Meissler Michael, Michael Meissler, Grosse-Siestrup, Christian, Fehrenberg, Claudia, Claudia Fehrenberg   +17 more
core   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

High‐Resolution MRI Revealed Different Etiology‐Specific Associations With Cerebral Infarction in Adult Moyamoya Vasculopathy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective High‐resolution MRI enables detailed assessment of intracranial vessel wall pathology in moyamoya vasculopathy. We aimed to classify adult moyamoya vasculopathy etiologies using high‐resolution MRI and to examine subtype‐specific associations between high‐resolution MRI features and ischemic infarction.
Guangsong Han, Jiachun Pan, Yuehui Hong, Xiaoyuan Fan, Ming Yao, Lixin Zhou, Yicheng Zhu, Feng Feng, Jun Ni   +8 more
wiley   +1 more source

Three-dimensional cell culture and tissue engineering in a T-CUP (tissue culture under perfusion) [PDF]

, 2007
The aim of this study was to develop and validate a simple and compact bioreactor system for perfusion cell seeding and culture through 3-dimensional porous scaffolds.
Früh, Jennifer-Annika, Timmins, Nicholas E., Heberer, Michael, Martin, Ivan, Scherberich, Arnaud, Jakob, Marcel   +5 more
core  

Perfusion-weighted FREE map using a bSSFP sequence at 3T with a flip angle of 70°.

, 2022
A temporal perfusion-weighted MIP is showing the maximal amplitude of the entire heart cycle for each voxel (a) and the perfusion-weighted map (b) is presenting the maximum median amplitude within the parenchyma ROI.
Frank Wacker (281957), Agilo Luitger Kern (14135251), Marcel Gutberlet (456685), Mike P. Wattjes (6239945), Norman Kornemann (14135254), Nima Mahmoudi (6534023), Jens Vogel-Claussen (313462), Andreas Voskrebenzev (4669540), Filip Klimeš (9928454), Julian Glandorf (9928451)   +9 more
core   +1 more source

Choroid Plexus Enlargement and USPIO‐Based Inflammatory Feature in Cerebral Small Vessel Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The choroid plexus (CP) is a key component of the blood–cerebrospinal fluid barrier (BCSFB), but its mechanism of action in cerebral small vessel disease (CSVD) remains unclear. This study investigated CP volume (CPV) alterations and their association with conventional imaging markers in CSVD and explored the underlying role of ...
Yongqiang Qu, Xiaoning Ren, Cuihua Fan, Chun Li, Lijie Zhang, Zaiqiang Zhang, Shaowu Li, Zhongmeng Lai, Bin Cai, Ying Fu, Yi Lin, Zhibao Zhu   +11 more
wiley   +1 more source