Results 1 to 10 of about 26,737 (217)

Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening [PDF]

Frontiers in Microbiology, 2019
Proteins that contain disulfide bonds mainly mature in the oxidative environment of the eukaryotic endoplasmic reticulum or the periplasm of Gram-negative bacteria. In E.
Alexandros Karyolaimos, Henry Ampah-Korsah, Tamara Hillenaar, Anna Mestre Borras, Katarzyna Magdalena Dolata, Susanne Sievers, Katharina Riedel, Robert Daniels, Jan-Willem de Gier   +8 more
doaj   +2 more sources

Highly efficient export of a disulfide‐bonded protein to the periplasm and medium by the Tat pathway using CyDisCo in Escherichia coli [PDF]

MicrobiologyOpen, 2023
High‐value heterologous proteins produced in Escherichia coli that contain disulfide bonds are almost invariably targeted to the periplasm via the Sec pathway as it, among other advantages, enables disulfide bond formation and simplifies downstream ...
Klaudia Arauzo‐Aguilera, Mirva J. Saaranen, Colin Robinson, Lloyd W. Ruddock   +3 more
doaj   +2 more sources

The Bacteroid Periplasm in Soybean Nodules Is an Interkingdom Symbiotic Space [PDF]

Molecular Plant-Microbe Interactions, 2017
The functional role of the periplasm of nitrogen-fixing bacteroids has not been determined. Proteins were isolated from the periplasm and cytoplasm of Bradyrhizobium diazoefficiens bacteroids and were analyzed using liquid chromatography tandem mass ...
Kent N. Strodtman, Severin E. Stevenson, James K. Waters, Thomas P. Mawhinney, Jay J. Thelen, Joseph C. Polacco, David W. Emerich   +6 more
doaj   +2 more sources

c-Type Cytochrome Assembly Is a Key Target of Copper Toxicity within the Bacterial Periplasm

mBio, 2015
In the absence of a tight control of copper entrance into cells, bacteria have evolved different systems to control copper concentration within the cytoplasm and the periplasm.
Anne Durand, Asma Azzouzi, Marie-Line Bourbon, Anne-Soisig Steunou, Sylviane Liotenberg, Akinori Maeshima, Chantal Astier, Manuela Argentini, Shingo Saito, Soufian Ouchane   +9 more
doaj   +2 more sources

Periplasmic protein quality control at atomic level in live cells [PDF]

Nature Communications
The periplasm of gram-negative bacteria facilitates critical functions, including nutrient uptake, cell wall metabolism, antibiotic resistance, and virulence.
Lisandro J. González, Francisco J. Hita, Letizia Pontoriero, Roberta Pierattelli, Andres Binolfi, Alejandro J. Vila   +5 more
doaj   +2 more sources

Cefoperazone induces esterase B expression by EstR and esterase B enhances cefoperazone activity at the periplasm

International Journal of Medical Microbiology, 2020
The occurrence of antibiotic resistance bacteria has become a major threat to public health. We have recently discovered a transcriptional activator that belongs to MarR family, EstR, and an esterase B (EstB) with a newly proposed de-arenethiolase ...
Neal Toewiwat, Wirongrong Whangsuk, Poonsakdi Ploypradith, Skorn Mongkolsuk, Suvit Loprasert   +4 more
doaj   +2 more sources

Reducing the Periplasmic Glutathione Content Makes Escherichia coli Resistant to Trimethoprim and Other Antimicrobial Drugs

Microbiology Spectrum, 2021
Although glutathione (GSH) has been shown to influence the antimicrobial effects of many kinds of antibiotics, little is known about its role in relation to trimethoprim (TMP), a widely used antifolate. In this study, several genes related to glutathione
Yajing Song, Zhen Zhou, Jing Gu, Junmei Yang, Jiaoyu Deng   +4 more
doaj   +1 more source

How Teichoic Acids Could Support a Periplasm in Gram-Positive Bacteria, and Let Cell Division Cheat Turgor Pressure

Frontiers in Microbiology, 2021
The cytoplasm of bacteria is maintained at a higher osmolality than the growth medium, which generates a turgor pressure. The cell membrane (CM) cannot support a large turgor, so there are two possibilities for transferring the pressure to the ...
Harold P. Erickson
doaj   +1 more source

Specific N-terminal amino acids potentiate the periplasmic expression of single-chain variable fragments in Escherichia coli

BioTechniques, 2023
Single-chain variable fragments (ScFvs) are important in therapy, diagnosis and research because of their elevated antigen affinity and low immunogenicity.
Yoshiro Hanyu, Mieko Kato
doaj   +1 more source

Vacuuming the Periplasm [PDF]

Journal of Bacteriology, 2005
Ever since their discovery multidrug resistance (MDR) transporters have provided a plethora of fascinating and challenging topics for fundamental and applied research. Recognized first as a cause of resistance of tumor cells to anticancer agents (reviewed in reference 5), they were later discovered in all living organisms, from bacteria to humans.
Olga, Lomovskaya, Maxim, Totrov
openaire   +2 more sources