Results 11 to 20 of about 26,737 (217)
BioTechniques, 2022 High-yield expression of quality antibody fragments is indispensable for research and diagnosis. Most recombinant antibody fragments are expressed in Escherichia coli using liquid cultures; however, their yields and quality are often poor.
Yoshiro Hanyu, Mieko Kato
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Osmoregulated Periplasmic Glucans [PDF]
EcoSal Plus, 2017 Among all the systems developed by enterobacteria to face osmotic stress, only osmoregulated periplasmic glucans (OPGs) were found to be modulated during osmotic fluxes. First detected in 1973 by E.P. Kennedy’s group in a study of phospholipid turnover in Escherichia coli , OPGs have been shown across alpha, beta ...
Bontemps-Gallo, Sébastien +2 more
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Pushing the Envelope: The Mysterious Journey Through the Bacterial Secretory Machinery, and Beyond
Frontiers in Microbiology, 2021 Gram-negative bacteria are contained by an envelope composed of inner and outer-membranes with the peptidoglycan (PG) layer between them. Protein translocation across the inner membrane for secretion, or insertion into the inner membrane is primarily ...
Luca A. Troman, Ian Collinson
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Frontiers in Bioengineering and Biotechnology, 2020 Recently, we engineered a tunable rhamnose promoter-based setup for the production of recombinant proteins in E. coli. This setup enabled us to show that being able to precisely set the production rate of a secretory recombinant protein is critical to ...
Alexandros Karyolaimos +8 more
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The NtrYX Two-Component System Regulates the Bacterial Cell Envelope
mBio, 2020 Activity of the NtrYX two-component system has been associated with important processes in diverse bacteria, ranging from symbiosis to nitrogen and energy metabolism.
Kimberly C. Lemmer +9 more
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A Novel Salmonella Periplasmic Protein Controlling Cell Wall Homeostasis and Virulence
Frontiers in Microbiology, 2021 Horizontal gene transfer has shaped the evolution of Salmonella enterica as pathogen. Some functions acquired by this mechanism include enzymes involved in peptidoglycan (PG) synthesis and remodeling.
Juan J. Cestero +5 more
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eLife, 2022 The peptidoglycan cell wall is a predominant structure of bacteria, determining cell shape and supporting survival in diverse conditions. Peptidoglycan is dynamic and requires regulated synthesis of new material, remodeling, and turnover – or autolysis –
Anna Isabell Weaver +7 more
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The ColM Family, Polymorphic Toxins Breaching the Bacterial Cell Wall
mBio, 2018 Bacteria host an arsenal of antagonism-mediating molecules to combat for ecologic space. Bacteriocins represent a pivotal group of secreted antibacterial peptides and proteins assisting in this fight, mainly eliminating relatives.
Maarten G. K. Ghequire +2 more
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Frontiers in Microbiology, 2021 Bacterial C-type haem-copper oxidases in the cbb3 family are widespread in microaerophiles, which exploit their high oxygen-binding affinity for growth in microoxic niches. In microaerophilic pathogens, C-type oxidases can be essential for infection, yet
Nitanshu Garg +6 more
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Engineered fluorescent proteins illuminate the bacterial periplasm
Computational and Structural Biotechnology Journal, 2012 The bacterial periplasm is of special interest whenever cell factories are designed and engineered. Recombinantely produced proteins are targeted to the periplasmic space of Gram negative bacteria to take advantage of the authentic N-termini, disulfide ...
Thorben Dammeyer, Philip Tinnefeld
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