Results 191 to 200 of about 26,737 (217)
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Periplasmic Chaperones and Prolyl Isomerases
EcoSal Plus, 2018The biogenesis of periplasmic and outer membrane proteins (OMPs) in Escherichia coli is assisted by a variety of processes that help with their folding and transport to their final destination in the cellular envelope.
Stull, Frederick +2 more
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1994
Publisher Summary This chapter discusses periplasm. It addresses current views on the structural and functional organization of the periplasm. Periplasm is a concept pertaining to the envelope of Gram-negative bacteria, and comprises the molecules and ions that are localized within the space between the inner membrane (IM) and outer membrane (OM ...
Manfred E. Bayer, Margret H. Bayer
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Publisher Summary This chapter discusses periplasm. It addresses current views on the structural and functional organization of the periplasm. Periplasm is a concept pertaining to the envelope of Gram-negative bacteria, and comprises the molecules and ions that are localized within the space between the inner membrane (IM) and outer membrane (OM ...
Manfred E. Bayer, Margret H. Bayer
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Selection for a periplasmic factor improving phage display and functional periplasmic expression
Nature Biotechnology, 1998The efficiency of both phage display in Escherichia coli and periplasmic expression of recombinant proteins may be limited by the same periplasmic folding steps. To search for E. coli factors that improve the efficiency of both procedures, a library of E. coli proteins was coexpressed in a phagemid vector that contained a poorly folding single-chain Fv
H, Bothmann, A, Plückthun
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Microbial Drug Resistance, 1996
ABSTRACT A brief overview of thin sections of cryopreserved walls from select eubacteria will be presented to suggest that all bacteria have functional periplasms, but that these are not necessarily confined to a periplasmic space such as found in typical gram-negative bacteria.
T J, Beveridge, J L, Kadurugamuwa
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ABSTRACT A brief overview of thin sections of cryopreserved walls from select eubacteria will be presented to suggest that all bacteria have functional periplasms, but that these are not necessarily confined to a periplasmic space such as found in typical gram-negative bacteria.
T J, Beveridge, J L, Kadurugamuwa
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Control of Expression of a Periplasmic Nickel Efflux Pump by Periplasmic Nickel Concentrations
BioMetals, 2005There is accumulating evidence that transenvelope efflux pumps of the resistance, nodulation, cell division protein family (RND) are excreting toxic substances from the periplasm across the outer membrane directly to the outside. This would mean that resistance of Gram-negative bacteria to organic toxins and heavy metals is in fact a two-step process ...
Gregor, Grass +2 more
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Structural Biology of Periplasmic Chaperones
2009Proteins often require specific helper proteins, chaperones, to assist with their correct folding and to protect them from denaturation and aggregation. The cell envelope of Gram-negative bacteria provides a particularly challenging environment for chaperones to function in as it lacks readily available energy sources such as adenosine 5' triphosphate (
William J, Allen +2 more
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The charasome periplasmic space
Protoplasma, 1981Charasome structure, with special attention to the periplasmic space, is examined using various techniques. The periplasmic space appears electron lucent in sections stained with 2% aqueous uranyl acetate and lead citrate. When sections are stained with saturated methanolic uranyl acetate a densely staining central core can be seen within the ...
V. R. Franceschi, W. J. Lucas
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Trends in Biotechnology, 2001
Researchers at the University of Texas (Austin, TX, USA) have developed a new technique for screening proteins expressed in bacterial periplasm that avoids the need for protein display (Nature Biotechnology, June issue). Periplasmic expression with cytometric screening (PECS) screens proteins in the periplasm by incubating them with a fluorescent ...
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Researchers at the University of Texas (Austin, TX, USA) have developed a new technique for screening proteins expressed in bacterial periplasm that avoids the need for protein display (Nature Biotechnology, June issue). Periplasmic expression with cytometric screening (PECS) screens proteins in the periplasm by incubating them with a fluorescent ...
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Periplasmic Screening for Artificial Metalloenzymes
2016Artificial metalloenzymes represent an attractive means of combining state-of-the-art transition metal catalysis with the benefits of natural enzymes. Despite the tremendous recent progress in this field, current efforts toward the directed evolution of these hybrid biocatalysts mainly rely on the laborious, individual purification of protein variants ...
Jeschek, Markus +2 more
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2014
Before 1980, just two types of bacterial nitrate reductase were widely recognized: these were the soluble, cytoplasmic nitrate reductases involved in nitrate assimilation and the membrane-associated respiratory nitrate reductases. It rapidly became apparent that, in contrast to the more conserved components of the membrane-associated respiratory ...
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Before 1980, just two types of bacterial nitrate reductase were widely recognized: these were the soluble, cytoplasmic nitrate reductases involved in nitrate assimilation and the membrane-associated respiratory nitrate reductases. It rapidly became apparent that, in contrast to the more conserved components of the membrane-associated respiratory ...
openaire +1 more source