Results 21 to 30 of about 26,737 (217)
β-lactamase (Bla) Reporter-based System to Study Flagellar Type 3 Secretion in Salmonella
Bio-Protocol, 2023 Export of type 3 secretion (T3S) substrates is traditionally evaluated using trichloroacetic acid (TCA) precipitation of cultured cell supernatants followed by western blot analysis of the secreted substrates.
Fabienne Chevance, Kelly Hughes
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Frontiers in Microbiology, 2020 Bacterial resistance to antibiotics is a major concern worldwide, leading to an extensive search for alternative drugs. Promising candidates are antimicrobial peptides (AMPs), innate immunity molecules, shown to be highly efficient against multidrug ...
Gal Kapach +7 more
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A periplasm in Bacillus subtilis [PDF]
Journal of Bacteriology, 1995 The possibility of there being a periplasm in Bacillus subtilis, in the distinct cell compartment bounded by the cytoplasmic membrane and the thick cell wall, has been investigated quantitatively and qualitatively. Cytoplasmic, membrane, and protoplast supernatant fractions were obtained from protoplasts which were prepared isotonically from cells ...
R, Merchante, H M, Pooley, D, Karamata
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Defining the function of OmpA in the Rcs stress response
eLife, 2020 OmpA, a protein commonly found in the outer membrane of Gram-negative bacteria, has served as a paradigm for the study of β-barrel proteins for several decades.
Kilian Dekoninck +10 more
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Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli
Frontiers in Bioengineering and Biotechnology, 2021 Main reasons to produce recombinant proteins in the periplasm of E. coli rather than in its cytoplasm are to -i- enable disulfide bond formation, -ii- facilitate protein isolation, -iii- control the nature of the N-terminus of the mature protein, and -iv-
Alexandros Karyolaimos +1 more
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eLife, 2022 The cell envelope of Gram-negative bacteria consists of two membranes surrounding a periplasm and peptidoglycan layer. Molecular machines spanning the cell envelope depend on spatial constraints and load-bearing forces across the cell envelope and ...
Eric Mandela +13 more
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mSphere, 2020 Two-component systems control periplasmic Cu+ homeostasis in Gram-negative bacteria. In characterized systems such as Escherichia coli CusRS, upon Cu+ binding to the periplasmic sensing region of CusS, a cytoplasmic phosphotransfer domain of the sensor ...
Lorena Novoa-Aponte +3 more
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50 Years of Giese Reaction – a Personal View
Angewandte Chemie, EarlyView.The Giese‐Reaction celebrates its 50. Birthday, it is a cyclic radical chain reaction centered around C,C‐bond formation in a three component synthesis. This minireview speaks about the birth of the name‐reaction, explains how the complete understanding of its reaction mechanism stimulates new methods in organic synthesis, and why it can proceed in ...
Martin Spichty +4 more
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Nitrate and periplasmic nitrate reductases [PDF]
Chem. Soc. Rev., 2014 The nitrate anion is a simple, abundant and relatively stable species, yet plays a significant role in global cycling of nitrogen, global climate change, and human health. Although it has been known for quite some time that nitrate is an important species environmentally, recent studies have identified potential medical applications.
Courtney, Sparacino-Watkins +2 more
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Oxidative folding: recent developments
Biomolecular Concepts, 2011 Disulfide bond formation in proteins is an effective tool of both structure stabilization and redox regulation. The prokaryotic periplasm and the endoplasmic reticulum of eukaryotes were long considered as the only compartments for enzyme mediated ...
Szarka András, Bánhegyi Gábor
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