Results 41 to 50 of about 26,737 (217)

Energy coupling in bacterial periplasmic permeases [PDF]

Journal of Bacteriology, 1990
Bacterial active transport systems (permeases) can be broadly divided into two classes with regard to their mechanism of energy coupling: those energized by electrochemical ion gradients and those energized by substrate-level phosphorylation. The distribution of the permeases according to this classification is matched by their fundamentally different ...
G F, Ames, A K, Joshi
openaire   +2 more sources

Excess copper catalyzes protein disulfide bond formation in the bacterial periplasm but not in the cytoplasm [PDF]

, 2023
Stefanie S. Eben, James A. Imlay
openalex   +1 more source

Expanding the Toolbox for Inducible Protein Expression With Automation‐enabled Generation of Glycomimetics

Chemistry – A European Journal, EarlyView.
Inducible protein expression is a cornerstone of many aspects of industrial and molecular biotechnological processes. 2′‐fucosyl isopropyl‐β‐D‐thiogalactopyranose (2′F‐IPTG) and 2′‐fucosyl isobutyl‐C‐galactoside (2′F‐IBCG) mimics were explored for protein expression studies.
Ashley E. DeYong, Keevan C. Marion, Murat Ozturk, Sanjeeva Kumar Murali, Fatima Enam, Thomas J. Mansell, Nicola L. B. Pohl   +6 more
wiley   +1 more source

A robust fractionation method for protein subcellular localization studies in Escherichia coli

BioTechniques, 2019
Fractionation in Gram-negative bacteria is used to identify the subcellular localization of proteins, in particular the localization of exported recombinant proteins.
Gilles Malherbe, David Paul Humphreys, Emma Davé   +2 more
doaj   +1 more source

Engineered small metal‐binding protein tag improves the production of recombinant human growth hormone in the periplasm of Escherichia coli

FEBS Open Bio, 2020
Fusion proteins play an important role in the production of recombinant proteins in Escherichia coli. They are mostly used for cytoplasmic expression since they can be designed to increase the solubility of the target protein, which then can be easily ...
David A. Perez‐Perez, Elizeth Pioquinto‐Avila, Eder Arredondo‐Espinoza, Jose Ruben Morones‐Ramirez, Isaias Balderas‐Renteria, Xristo Zarate   +5 more
doaj   +1 more source

Disulfide-compatible phage-assisted continuous evolution in the periplasmic space

Nature Communications, 2021
The directed evolution of antibodies yields important tools for research and therapy. Here the authors develop a periplasmic phage-assisted continuous evolution platform for improvement of protein-protein interactions in the disulfidecompatible E.
Mary S. Morrison, Tina Wang, Aditya Raguram, Colin Hemez, David R. Liu   +4 more
doaj   +1 more source

Folding mechanisms of periplasmic proteins

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2014
More than one fifth of the proteins encoded by the genome of Escherichia coli are destined to the bacterial cell envelope. Over the past 20years, the mechanisms by which envelope proteins reach their three-dimensional structure have been intensively studied, leading to the discovery of an intricate network of periplasmic folding helpers whose members ...
Goemans, Camille, Denoncin, Katleen, Collet, Jean-François   +2 more
openaire   +2 more sources

Engineered Probiotics: The Next‐Generation Therapeutics to Combat Antibiotic‐Resistant Bacterial Infections

Food Frontiers, EarlyView.
Engineered probiotics secretes fusion proteins which potentially neutralized the toxins secreted by other microbial communities. ABSTRACT On a global scale, the escalating burden of infectious diseases, predominantly attributed to bacterial pathogens, especially drug‐resistant strains, has progressed into a critical concern for clinical management and ...
Indu Singh, Raz Abdulqadir, Indira Kumari Verma, Yashika Awasthi, Arun Ratn, Juhi Sharma, Manmeet Rawat, Megh Singh Dhakad, Jasbir Dalal, Yash Gupta, Bhisham Narayan Singh, Divakar Sharma   +11 more
wiley   +1 more source

Teichoic acids in the periplasm and cell envelope of Streptococcus pneumoniae

eLife
Teichoic acids (TA) are linear phospho-saccharidic polymers and important constituents of the cell envelope of Gram-positive bacteria, either bound to the peptidoglycan as wall teichoic acids (WTA) or to the membrane as lipoteichoic acids (LTA).
Mai Nguyen, Elda Bauda, Célia Boyat, Cédric Laguri, Céline Freton, Anne Chouquet, Benoit Gallet, Morgane Baudoin, Yung-Sing Wong, Christophe Grangeasse, Christine Moriscot, Claire Durmort, André Zapun, Cecile Morlot   +13 more
doaj   +1 more source

From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity

Small, EarlyView.
Antimicrobial peptides (AMPs) are promising candidates for next‐generation antibiotics, acting through mechanisms such as membrane disruption and intracellular targeting. This review examines how variations in bacterial membrane composition critically influence AMP activity.
Paolo Rossetti, Marius F.W. Trollmann, Christina Wichmann, Thomas Gutsmann, Christian Eggeling, Rainer A. Böckmann   +5 more
wiley   +1 more source