Results 71 to 80 of about 26,737 (217)

Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region

Microbial Cell Factories, 2020
Background Recombinant proteins are often engineered with an N-terminal signal peptide, which facilitates their secretion to the oxidising environment of the periplasm (gram-negative bacteria) or the culture supernatant (gram-positive bacteria).
Kiavash Mirzadeh, Patrick J. Shilling, Rageia Elfageih, Alister J. Cumming, Huanhuan L. Cui, Maja Rennig, Morten H. H. Nørholm, Daniel O. Daley   +7 more
doaj   +1 more source

Protein Target Highlights in CASP16: Insights From the Structure Providers

Proteins: Structure, Function, and Bioinformatics, Volume 94, Issue 1, Page 25-50, January 2026.
ABSTRACT This article presents an in‐depth analysis of selected CASP16 targets, with a focus on their biological and functional significance. The authors highlight the most relevant features of the target proteins and discuss how well these were reproduced in the submitted predictions.
Leila T. Alexander, Océane M. Follonier, Andriy Kryshtafovych, Kim Abesamis, Sabrina Bibi‐Triki, Henry G. Box, Cécile Breyton, Françoise Bringel, Loic Carrique, Alessio d'Acapito, Gang Dong, Rebecca DuBois, Deborah Fass, Juliana Martinez Fiesco, Daniel R. Fox, Jonathan M. Grimes, Rhys Grinter, Matthew Jenkins, Roman Kamyshinsky, Jeremy R. Keown, Gerald Lackner, Michael Lammers, Shiheng Liu, Andrew L. Lovering, Tomas Malinauskas, Benoît Masquida, Gottfried J. Palm, Christian Siebold, Tiantian Su, Ping Zhang, Z. Hong Zhou, Krzysztof Fidelis, Maya Topf, John Moult, Torsten Schwede   +34 more
wiley   +1 more source

Characterization of YdgH: a mediator of beta-lactam susceptibility in Enterobacterales

Microbiology Spectrum
Beta-lactam antibiotics are often the treatment of choice for serious bacterial infections. In a previous screen for novel genetic mediators affecting beta-lactam susceptibility, we discovered that deletion of ydgH, a conserved gene of unknown function ...
Jacob E. Lazarus, Matthew K. Waldor, David C. Hooper   +2 more
doaj   +1 more source

OmpA_C‐Like Domain of PorE Is Essential for PorE Function in the Type IX Secretion System (T9SS) of Porphyromonas gingivalis and Some T9SS Cargo Proteins Are Secreted in a PorE‐Independent Manner

Microbiology and Immunology, Volume 70, Issue 1, Page 15-26, January 2026.
ABSTRACT The periodontal pathogen Porphyromonas gingivalis secretes gingipains, highly hydrolytic proteases, to the cell surface or external environment via a type IX secretion system (T9SS). PorE is one of the T9SS component proteins essential for gingipain secretion. It consists of four domains: TPR, WD40, CRD, and OmpA_C‐like.
Takashi Tominaga, Hideharu Yukitake, Paul D. Veith, Dhana G. Gorasia, Takao Ayuse, Takuro Sanuki, Eric C. Reynolds, Koji Nakayama, Mariko Naito, Mikio Shoji   +9 more
wiley   +1 more source

Cross‐Feeding of Carbon and Nitrogen Between Aquificales and Thermus in Hot Springs

Environmental Microbiology, Volume 28, Issue 1, January 2026.
Pampinifervens and Thermus populations are co‐distributed in hot springs in Yellowstone and Iceland. Genome reconstruction suggests Pampinifervens Flu05 cross feeds Thermus organic carbon and nitrogen, a phenomenon that was verified via co‐culture studies.
Lisa M. Keller, Daniel R. Colman, Andri Stefánsson, Eric S. Boyd   +3 more
wiley   +1 more source

Architecture and Assembly of Periplasmic Flagellum

Microbiology Spectrum, 2019
ABSTRACT Periplasmic flagella are complex nanomachines responsible for distinctive morphology and motility of spirochetes. Although bacterial flagella have been extensively studied for several decades in the model systems Escherichia coli and Salmonella enterica , our understanding ...
Yunjie, Chang, Jun, Liu
openaire   +3 more sources

A Cytochrome c‐Containing Periplasmic Nitrate Reductase in the Acetogen Sporomusa ovata

Environmental Microbiology, Volume 28, Issue 1, January 2026.
Sporomusa ovata, a cytochrome‐containing, acetogenic bacterium, conserves energy by reduction of carbon dioxide to acetate. Nitrate serves as additional electron acceptor and is reduced to ammonium, resulting in increased growth and reduced acetate production.
Lara M. Waschinger, Anja Poehlein, Rolf Daniel, Florian P. Rosenbaum, Volker Müller   +4 more
wiley   +1 more source

Comparison of Predatory Phenotypes and Genotypes Between Bdellovibrio sp. BIS2 and Bacteriovorax sp. HI3 Isolated From the Same Freshwater Environment

Environmental Microbiology, Volume 28, Issue 1, January 2026.
Bdellovibrio and like organisms (BALOs) have gained attraction owing to their ecological and practical significance. This study comparatively investigated two BALO strains from the same freshwater pond, Bdellovibrio sp. BIS2 and Bacteriovorax sp. HI3, and revealed significant variation in their predatory behaviours and associated genetic profiles ...
Tomomi Sugiyama, Tsubasa Kojima, Naoki A. Uemura, Daisuke Nakane, Hideo Dohra, Daisuke Inoue, Michihiko Ike   +6 more
wiley   +1 more source

A Periplasmic Antimicrobial Peptide-Binding Protein Is Required for Stress Survival in Vibrio cholerae

Frontiers in Microbiology, 2019
Vibrio cholerae must sense and respond appropriately to stresses encountered in the aquatic environment and the human host. One stress encountered in both environments is exposure to antimicrobial peptides (AMPs), produced as a part of the innate immune ...
Jessica Saul-McBeth, Jyl S. Matson
doaj   +1 more source

Impact of Copper on the Physiology and Transcriptome of Methylosinus Trichosporium OB3b Grown on Either Methane or Methanol

Environmental Microbiology, Volume 28, Issue 1, January 2026.
Methylosinus trichosporium OB3b can grow on methanol in the absence but not presence of copper due: (1) overgeneration of reducing power from methanol oxidation; (2) formate accumulation leading to acidification of the growth medium and inactivation of formate dehydrogenase; and (3) lack of expression of potential oxidative stress defence genes ...
Peng Peng, Jeremy D. Semrau
wiley   +1 more source