Disulfide-compatible phage-assisted continuous evolution in the periplasmic space [PDF]
Nature Communications, 2021 The directed evolution of antibodies yields important tools for research and therapy. Here the authors develop a periplasmic phage-assisted continuous evolution platform for improvement of protein-protein interactions in the disulfidecompatible E.
Mary S. Morrison +4 more
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Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space [PDF]
Nature Communications, 2018 No structural data for the bacterial type IX secretion system (T9SS) are available so far. Here, the authors present the crystal structures of the periplasmic domains from two major T9SS components PorM and GldM, which span most of the periplasmic space,
Philippe Leone +8 more
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Functional Properties of Mouse Chitotriosidase Expressed in the Periplasmic Space of Escherichia coli. [PDF]
PLoS ONE, 2016 Chitotriosidase (Chit1) is an enzyme associated with various diseases, including Gaucher disease, chronic obstructive pulmonary disease, Alzheimer disease and cystic fibrosis.
Masahiro Kimura +13 more
doaj +6 more sources
Author Correction: Disulfide-compatible phage-assisted continuous evolution in the periplasmic space [PDF]
Nature Communications, 2021 Mary S. Morrison +4 more
doaj +5 more sources
Effects of adding poly-histidine tag on stability, antimicrobial activity and safety of recombinant buforin I expressed in periplasmic space of Escherichia coli [PDF]
Scientific Reports, 2023 The lack of cost-effective methods for producing antimicrobial peptides has made it impossible to use their high potential as a new and powerful class of antimicrobial agents. In recent years, extensive research has been conducted to decrease the cost of
Sahar Roshanak +5 more
doaj +4 more sources
Overexpression of Recombinant Human Beta Interferon (rhINF-β) in Periplasmic Space of Escherichia coli. [PDF]
Iran J Pharm Res, 2014 Human Interferon β (INF-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. In this study an expression vector was designed and constructed for expression of human INF-β-1b either in shake flasks or bench top bioreactor.
Morowvat MH +4 more
europepmc +5 more sources
Thioredoxin-linked "Thiol Peroxidase" from Periplasmic Space of Escherichia coli [PDF]
Journal of Biological Chemistry, 1995 Three different molecular masses (24, 22, and 20 kDa) of antioxidant proteins were purified in Escherichia coli. These proteins exhibited the preventive effects against the inactivation of glutamine synthetase activity and the cleavage of DNA by a metal-catalyzed oxidation system capable of generating reactive oxygen species.
Mee‐Kyung Cha, Ha-Kun Kim, Il-Han Kim
openalex +4 more sources
The Bacteroid Periplasm in Soybean Nodules Is an Interkingdom Symbiotic Space [PDF]
Molecular Plant-Microbe Interactions, 2017 The functional role of the periplasm of nitrogen-fixing bacteroids has not been determined. Proteins were isolated from the periplasm and cytoplasm of Bradyrhizobium diazoefficiens bacteroids and were analyzed using liquid chromatography tandem mass ...
Kent N. Strodtman +6 more
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Distribution of Alkaline Phosphatase Within the Periplasmic Space of Gram-Negative Bacteria [PDF]
Journal of Bacteriology, 1972 Both reaction-product localization and ferritin-coupled antibody studies have shown that alkaline phosphatase is evenly distributed throughout the peri-plasmic space of Escherichia coli and a marine pseudomonad. This space is not locally enlarged except in cases where plasmolysis has occurred.
T J MacAlister +4 more
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Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space. [PDF]
Comput Struct Biotechnol J, 2022 Okoye JC +5 more
europepmc +2 more sources