Results 181 to 190 of about 23,652 (216)

Colicins are not transiently accumulated in the periplasmic space before release from colicinogenic cells

Biology of the Cell, 1984
Colicins are released into the spent medium from colicinogenic cells. The pathway of release has been investigated in this study. The localization in producing cells of colicins A, E3 and of cloacin DF13 has been determined at various times after mitomycin C addition: no transient accumulation in the periplasmic space of colicinogenic E.
D, Cavard, A, Bernadac, J M, Pages, C, Lazdunski   +3 more
openaire   +2 more sources

Relocation of dehydroquinate dehydratase to the periplasmic space improves dehydroshikimate production with Gluconobacter oxydans strain NBRC3244

Applied Microbiology and Biotechnology, 2021
3-Dehydroshikimate (3-DHS) is a key intermediate for the synthesis of various compounds, including the antiviral drug oseltamivir. The Gluconobacter oxydans strain NBRC3244 intrinsically oxidizes quinate to produce 3-dehydroquinate (3-DHQ) in the periplasmic space. Even though a considerable activity is detected in the recombinant G.
Kentaro Nakamura, Kakeru Nagaki, Minenosuke Matsutani, Osao Adachi, Naoya Kataoka, Yoshitaka Ano, Gunjana Theeragool, Kazunobu Matsushita, Toshiharu Yakushi   +8 more
openaire   +2 more sources

Targeting of Active Human Cytochrome P4501A1 (CYP1A1) to the Periplasmic Space of Escherichia coli

Biochemical and Biophysical Research Communications, 2000
Native human cytochrome P4501A1 (CYP1A1) was appended at its amino terminus to the secretory signal of Escherichia coli alkaline phosphatase. The chimeric P450 construct was placed under the transcriptional control of the native phoA promoter in a prokaryotic expression vector. Induction of the hemoprotein by heterologous expression in E.
M A, Kaderbhai, C C, Ugochukwu, D C, Lamb, S L, Kelly   +3 more
openaire   +2 more sources

High-yield purification of biosynthetic human growth hormone secreted in Escherichia coli periplasmic space

Journal of Chromatography A, 1999
A six-step, high-yield purification procedure for the preparation of clinical grade recombinant human growth hormone (rhGH) secreted in bacterial periplasmic space is described. Particular emphasis is given to hormone recovery yields and maximum contaminant host cell elimination.
J E, de Oliveira, C R, Soares, C N, Peroni, E, Gimbo, I M, Camargo, L, Morganti, M H, Bellini, R, Affonso, R R, Arkaten, P, Bartolini, M T, Ribela   +10 more
openaire   +2 more sources

Improving the stability of a foreign protein in the periplasmic space of Escherichia coli

Biochimie, 1988
An efficient expression/export vector comprising the entire phoS (phosphate binding protein) gene fused to a synthetic gene encoding the human growth hormone releasing factor (mhGRF) has recently been constructed [1]. The hybrid protein (PhoS-mhGRF) was exported to the periplasmic space. However, in this location proteolytic degradation occurred at the
Anba, Jamila, Bernadac, Alain, Lazdunski, Claude, Pagès, Jean-Marie   +3 more
openaire   +3 more sources

Studies on cyclic β-1,2-glucan obtained from periplasmic space ofRhizobium trifolii cells

Plant and Soil, 1982
A glycan was obtained from the periplasmic space ofRhizobium trifolii cells by saccharose-osmotic shock method. This glycan was composed of only β-1,2-linked glucose, having no end groups (reducing and non-reducing) and branching points. It was concluded that this glucan has a structure of unbranched cyclic form.
Mikiko Abe, Akinori Amemura, Shiro Higashi   +2 more
openaire   +1 more source

Expression, purification, and characterization of authentic mouse prolactin obtained in Escherichia coli periplasmic space

Biotechnology and Applied Biochemistry, 2012
AbstractProlactin (PRL) is a pleiotropic hormone produced by lactotroph cells of the anterior pituitary gland and is mainly related to lactation control and reproduction. Recombinant mouse prolactin (r‐mPRL), never obtained in its authentic form, can be very useful for research and tests in animal models, in which human prolactin (hPRL) is usually ...
Miriam F, Suzuki, Fernanda S, Arthuso, João E, Oliveira, Nélio A J, Oliveira, Herbert R, Goulart, Marcos V N, Capone, Maria Teresa C P, Ribela, Paolo, Bartolini, Carlos R J, Soares   +8 more
openaire   +2 more sources

Secretion of mature human interferon alpha 2 into the periplasmic space of Escherichia coli

Journal of Biotechnology, 1986
Abstract A series of plasmids which direct the synthesis of human interferon alpha 2 (HuIFN- α 2 ) under the control of the lipoprotein-lactose promoter has been constructed. Interferon was secreted across the cytoplasmic membrane of Escherichia coli when the mature protein was fused to the signal peptide of OmpA, a major outer membrane protein of
JoséL. Barbero, JoséM. Buesa, Miguel A. Peñalva, Agustín Pérez-Aranda, JoséL. García   +4 more
openaire   +1 more source

[Expression of recombinant h-FGF21 in periplasmic space of Escherichia coli].

Yao xue xue bao = Acta pharmaceutica Sinica, 2018
Fibroblast growth factor 21 (FGF21) is a novel metabolic regulator of glucose and lipid, which is safe, effective and independent on insulin. FGF21 is considered as a prospective anti-diabetic drug. The aim of this study was to express recombinant h-FGF21 in periplasmic space of Escherichia coli.
Shu-jie, Li, Xian-long, Ye, Qiang, Wu, Yin-hang, Yu, Dan, Yu, Gui-ping, Ren, De-shan, Li   +6 more
openaire   +1 more source

Identification of Bradyrhizobium Japonicum malonamidase E2 in the periplasmic space of soybean nodule bacteroids

Journal of Plant Physiology, 1996
Summary MalonamidaseE2 (MAE2) was found both in the plant cytosolic fraction and in the Bradyrhizobium japonicum bacteroids of soybean nodules, and was proposed to play an important role in the transfer of fixed nitrogen (NH 3 ) to plant cells. The enzyme in the cytosol was identical in its molecular size and was immunologically homologous with ...
Sang Won Kang, Yu Sam Kim
openaire   +1 more source