Folding and aggregation of beta-lactamase in the periplasmic space of Escherichia coli.
Journal of Biological Chemistry, 1990 High level expression of TEM beta-lactamase results in the accumulation of precursor and mature protein in the insoluble fraction of Escherichia coli. The mature polypeptide is sequestered in protein aggregates (inclusion bodies) located within the periplasmic space whereas the insoluble precursor is present in the cytoplasm.
Gregory A. Bowden, George Georgiou
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Protein folding in the periplasm in the absence of primary oxidant DsbA: modulation of redox potential in periplasmic space via OmpL porin [PDF]
The EMBO Journal, 2004 Disulfide bond formation in Escherichia coli is a catalyzed reaction accomplished by DsbA. We found that null mutations in a new porin gene, ompL, allowed a total bypass of the DsbA requirement for protein oxidation. These mutations acted as extragenic null suppressors for dsbA, and restored normal folding of alkaline phosphatase and relieved ...
Claire Dartigalongue +2 more
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Probing the mechanism of peptidoglycan amidase activation by FtsEX-EnvC [PDF]
mBioThe FtsEX-EnvC-AmiA/B system is a key component of the E. coli cell division machinery that directs breakage of the peptidoglycan layer during separation of daughter cells.
Jonathan Cook, Allister Crow
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Synthesis of Human Bone Morphogenetic Protein-2 (hBMP-2) in E. coli Periplasmic Space: Its Characterization and Preclinical Testing. [PDF]
Cells, 2021 Oliveira JE +9 more
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Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli. [PDF]
3 Biotech, 2016 Malik A.
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The Pseudomonas aeruginosa homeostasis enzyme AlgL clears the periplasmic space of accumulated alginate during polymer biosynthesis. [PDF]
J Biol Chem, 2022 Gheorghita AA +11 more
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.BETA.-Glucosidase and .BETA.-galactosidase from the periplasmic space of Rhizobium trifolii cells.
The Journal of General and Applied Microbiology, 1982 High amounts of β-glucosidase (MW 74, 000) and β-galactosidase (MW 122, 000) were isolated from the periplasmic space of Rhizobium trifolii 4S (infectious strain), compared with the cell homogenate. The characterization of β-glucosidase and β-galactosidase was determined.
Mikiko Abe, Shiro Higashi
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The Transport of Group 2 Capsular Polysaccharides across the Periplasmic Space in Escherichia coli [PDF]
Journal of Biological Chemistry, 2001 Carlos Arrecubieta +6 more
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Antibiotics, 2021 The cell envelope structure of Gram-negative bacteria is unique, composed of two lipid bilayer membranes and an aqueous periplasmic space sandwiched in between.
Olaniyi Alegun +4 more
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Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System [PDF]
Advanced Pharmaceutical Bulletin, 2016 Purpose: Recombinant human endostatin (rhEs) is an angiogenesis inhibitor which is used as a specific drug in the treatment of non-small-cell lung cancer.
Abbas Mohajeri +5 more
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