Results 31 to 40 of about 23,652 (216)

ENGINEERED FLUORESCENT PROTEINS ILLUMINATE THE BACTERIAL PERIPLASM

Computational and Structural Biotechnology Journal, 2012
The bacterial periplasm is of special interest whenever cell factories are designed and engineered. Recombinantely produced proteins are targeted to the periplasmic space of Gram negative bacteria to take advantage of the authentic N-termini, disulfide ...
Thorben Dammeyer, Philip Tinnefeld
doaj   +3 more sources

Influence of Foreign DNA Introduction and Periplasmic Expression of Recombinant Human Interleukin-2 on Hydrogen Peroxide Quantity and Catalase Activity in Escherichia coli [PDF]

Advanced Pharmaceutical Bulletin, 2013
Purpose: Oxidative stress is generated through imbalance between composing and decomposing of reactive oxygen species (ROS). This kind of stress was rarely discussed in connection with foreign protein production in Escherichia coli. Effect of cytoplasmic
Lena Mahmoudi Azar, Elnaz Mehdizadeh Aghdam, Farrokh Karimi, Babak Haghshenas, Abolfazl Barzegari, Parichehr Yaghmaei, Mohammad Saeid Hejazi   +6 more
doaj   +1 more source

How Teichoic Acids Could Support a Periplasm in Gram-Positive Bacteria, and Let Cell Division Cheat Turgor Pressure

Frontiers in Microbiology, 2021
The cytoplasm of bacteria is maintained at a higher osmolality than the growth medium, which generates a turgor pressure. The cell membrane (CM) cannot support a large turgor, so there are two possibilities for transferring the pressure to the ...
Harold P. Erickson
doaj   +1 more source

Spirochete Flagella and Motility

Biomolecules, 2020
Spirochetes can be distinguished from other flagellated bacteria by their long, thin, spiral (or wavy) cell bodies and endoflagella that reside within the periplasmic space, designated as periplasmic flagella (PFs).
Shuichi Nakamura
doaj   +1 more source

Effect of TAT-signaling fusion system along with co-expression of GroEL/ES chaperones on secretory expression of somatropin

BioTechnologia, 2020
Human growth hormone (somatropin) is one of the most widely used recombinant proteins that stimulates growth, cell reproduction, and cell regulation in humans.
Mohammad Rabbani, Razieh Ghasemi, Mohammad Reza Bagherinejad, Ali Jahanian   +3 more
doaj   +1 more source

The gram-negative bacterial periplasm: Size matters.

PLoS Biology, 2018
Gram-negative bacteria are surrounded by two membrane bilayers separated by a space termed the periplasm. The periplasm is a multipurpose compartment separate from the cytoplasm whose distinct reducing environment allows more efficient and diverse ...
Samuel I Miller, Nina R Salama
doaj   +1 more source

Construction and periplasmic expression of a bispecific tandem scFv for dual targeting of immune checkpoints

Advanced Biomedical Research, 2023
Background: Immune checkpoints are molecules that act as regulators of immune system pathways. However, some tumor cells can express the ligands of immune checkpoints to escape from antitumor immune responses. Some agents, such as antibodies, can inhibit
Amirreza Rashti, Vajihe Akbari
doaj   +1 more source

Effect of Twine-arginine Translocation-signaling Fusion System and Chaperones Co-expression on Secretory Expression of Somatropin

Advanced Biomedical Research, 2018
Background: Twine-arginine translocation (TAT) system is one of the exporting systems in Escherichia coli which could transport fully/semi-correctly folded proteins outside the reductive cytoplasmic space.
Mohammad Reza Bagherinejad, Hamid Mir-Mohammad Sadeghi, Daryoush Abedi, Fateme Moazen, Mohammad Rabbani   +4 more
doaj   +1 more source

Electron Flow From the Inner Membrane Towards the Cell Exterior in Geobacter sulfurreducens: Biochemical Characterization of Cytochrome CbcL

Frontiers in Microbiology, 2022
Exoelectrogenic microorganisms are in the spotlight due to their unique respiratory mechanisms and potential applications in distinct biotechnological fields, including bioremediation, bioenergy production and microbial electrosynthesis.
Jorge M. A. Antunes, Jorge M. A. Antunes, Marta A. Silva, Marta A. Silva, Carlos A. Salgueiro, Carlos A. Salgueiro, Leonor Morgado, Leonor Morgado   +7 more
doaj   +1 more source

Cloning, optimization of periplasmic expression and purification of recombinant granulocyte macrophage-stimulating factor in Escherichia coli BL21 (DE3)

Advanced Biomedical Research, 2019
Background: Molgramostim, a nonglycosylated version of recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF), can be produced in a high level by Escherichia coli.
Elham Taherian, Elmira Mohammadi, Ali Jahanian-Najafabadi, Fatemeh Moazen, Vajihe Akbari   +4 more
doaj   +1 more source