Results 41 to 50 of about 23,652 (216)
CONSTRUCTION OF RECOMBINANT PLASMIDS FOR PERIPLASMIC EXPRESSION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI UNDER T7 AND LAC PROMOTERS [PDF]
Journal of Sciences, Islamic Republic of Iran, 2003 In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7 ...
A. Zomorodipour
doaj
Microbial Cell Factories, 2010 Background Escherichia coli as a frequently utilized host organism for recombinant protein production offers different cellular locations with distinct qualities.
Paal Michael +3 more
doaj +1 more source
A Positive Selection for Nucleoside Kinases in E. coli. [PDF]
PLoS ONE, 2016 Engineering heterologous nucleoside kinases inside E. coli is a difficult process due to the integral role nucleosides play in cell division and transcription.
Nirav Y Shelat +2 more
doaj +1 more source
Lipoprotein Lpp regulates the mechanical properties of the E. coli cell envelope
Nature Communications, 2020 Lipoprotein Lpp provides a covalent crosslink between the outer membrane and the peptidoglycan in E. coli. Here, the authors use atomic force microscopy to show that Lpp contributes to cell envelope stiffness by covalently connecting the two layers and ...
Marion Mathelié-Guinlet +3 more
doaj +1 more source
In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS
FEBS Letters, EarlyView.We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka +11 more
wiley +1 more source
The Cell Envelope Structure of Cable Bacteria
Frontiers in Microbiology, 2018 Cable bacteria are long, multicellular micro-organisms that are capable of transporting electrons from cell to cell along the longitudinal axis of their centimeter-long filaments.
Rob Cornelissen +21 more
doaj +1 more source
Multivalent Protein Nanorings for Broad and Potent SARS‐CoV‐2 Neutralization
Advanced Healthcare Materials, EarlyView.A protein‐only, modular multivalent nanoscaffold displaying 20 anchor points, decorated with two different binders (10 of each), targeting the SARS‐CoV‐2 receptor‐binding domain is presented. The construct self‐assembles into stable, biocompatible, homogeneous nanoparticles, exhibit synergistic binding with fM IC50 values. It also detects spike at 9 ng
Molood Behbahanipour +11 more
wiley +1 more source
Twin arginine translocation system in secretory expression of recombinant human growth hormone
Research in Pharmaceutical Sciences, 2016 Recombinant protein production in E. coli has several advantages over other expression systems. Misfolding, inclusion body formation, and lack of eukaryotic post translational modification are the most disadvantages of this system. Exporting of correctly
Mohammad Reza Bagherinejad +5 more
doaj +1 more source
Secretion of Bacillus subtilis -Amylase in the Periplasmic Space of Escherichia coli
Microbiology, 1987 The Bacillus subtilis alpha-amylase structural gene (amyE) lacking its own signal peptide coding sequence was joined to the end of the Escherichia coli alkaline phosphatase (phoA) signal peptide coding sequence by using the technique of oligonucleotide-directed site-specific deletion. On induction of the phoA promoter, the B. subtilis alpha-amylase was
K, Tachibana +7 more
openaire +2 more sources
Advanced Sensor Research, EarlyView.This Review focuses on assessing and providing perspective on the field of rationally‐designed optical sensors constructed with single‐walled carbon nanotubes. The literature is reviewed and evaluated for SWCNT‐based sensors constructed with biomolecular recognition elements, including proteins, peptides, and oligonucleotides, as well as their methods ...
Amelia K. Ryan +4 more
wiley +1 more source