Results 51 to 60 of about 23,652 (216)

Dynamics of Intact MexAB-OprM Efflux Pump: Focusing on the MexA-OprM Interface

Scientific Reports, 2017
Antibiotic efflux is one of the most critical mechanisms leading to bacterial multidrug resistance. Antibiotics are effluxed out of the bacterial cell by a tripartite efflux pump, a complex machinery comprised of outer membrane, periplasmic adaptor, and ...
Cesar A. López, Timothy Travers, Klaas M. Pos, Helen I. Zgurskaya, S. Gnanakaran   +4 more
doaj   +1 more source

Computational prediction of secreted proteins in gram-negative bacteria

Computational and Structural Biotechnology Journal, 2021
Gram-negative bacteria harness multiple protein secretion systems and secrete a large proportion of the proteome. Proteins can be exported to periplasmic space, integrated into membrane, transported into extracellular milieu, or translocated into ...
Xinjie Hui, Zewei Chen, Junya Zhang, Moyang Lu, Xuxia Cai, Yuping Deng, Yueming Hu, Yejun Wang   +7 more
doaj   +1 more source

Increasing the succinate yield in Actinobacillus succinogenes by fumigation of H2 in a bioreactor

Biofuels, Bioproducts and Biorefining, EarlyView.
Abstract The microbial conversion of CO2 into value‐added chemicals offers a promising and scalable route for sustainable bioproduction, particularly under mild reaction conditions and with technically straightforward reactor configurations. Actinobacillus succinogenes, a natural succinate producer, can fix CO2 through anaplerotic carboxylation but its
Julian Tix, Gustav Zimpel, Roland Ulber, Nils Tippkötter   +3 more
wiley   +1 more source

Development of a Jacketed Breathable Shake Flask With Process Monitoring, Control, and Bioreactor‐Like Performance

Biotechnology and Bioengineering, EarlyView.
This article presents the design and evaluation of a gas‐control jacket integrated with a breathable shake flask, enabling precise regulation of dissolved oxygen and carbon dioxide to improve scalability, process control, and culture performance in shaking bioreactors.
Vikash Kumar, Chad Sundberg, Venkatesh Srinivasan, Aaron Thole, Michael Tolosa, Govind Rao   +5 more
wiley   +1 more source

A Novel Periplasmic Protein, VrpA, Contributes to Efficient Protein Secretion by the Type III Secretion System in Xanthomonas spp.

Molecular Plant-Microbe Interactions, 2015
Efficient secretion of type III effector proteins from the bacterial cytoplasm to host cell cytosol via a type III secretion system (T3SS) is crucial for virulence of plant-pathogenic bacterium.
Xiaofeng Zhou, Xiufang Hu, Jinyun Li, Nian Wang   +3 more
doaj   +1 more source

Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking

Mass Spectrometry Reviews, EarlyView.
ABSTRACT The exploration of protein structure and function stands at the forefront of life science and represents an ever‐expanding focus in the development of proteomics. As mass spectrometry (MS) offers readout of protein conformational changes at both the protein and peptide levels, MS‐based structural proteomics is making significant strides in the
Haiyan Lu, Zexin Zhu, Lauren Fields, Hua Zhang, Lingjun Li   +4 more
wiley   +1 more source

Tumor Cell Migration May Be an Inherent “Foraging” Behavior

Medicine Advances, EarlyView.
Tumor cells gradually form pseudopodia, migrate to necrotic cells, make contact with them, and absorb necrotic cell debris. During this migration, small vesicles formed by dying tumor cells also gradually migrate toward living tumor cells. Once the nutrients from the necrotic cells have been completely absorbed, the living tumor cells will leave ...
Fuqian Zhao, Hongmei Niu, Lusi Zhang, Daofu Shen, Lei Wang   +4 more
wiley   +1 more source

Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli

Microbial Cell Factories, 2011
Background The eukaryotic RNase ribonuclease/angiogenin inhibitors (RI) are a protein group distinguished by a unique structure - they are composed of hydrophobic leucine-rich repeat motifs (LRR) and contain a high amount of reduced cysteine residues ...
Neubauer Peter, Šiurkus Juozas
doaj   +1 more source

Proteome Analysis of Corynebacterium diphtheriae–Macrophage Interaction

PROTEOMICS, EarlyView.
ABSTRACT Contact of Corynebacterium diphtheriae with macrophages induces adaptations on both bacterial and cellular sides. The study presented here was aiming to shed light on the simultaneous intracellular adaptation of the bacteria and changes in the proteome of the phagocytes in response to the internalization of C. diphtheriae.
Luca Musella, Jens Möller, Christopher Lischer, Julio Vera‐Gonzalez, Jörg Hofmann, Lisa Ott, Andreas Burkovski   +6 more
wiley   +1 more source

Bacteroides fragilis requires the ferrous‐iron transporter FeoAB and the CobN‐like proteins BtuS1 and BtuS2 for assimilation of iron released from heme

MicrobiologyOpen, 2019
The intestinal commensal and opportunistic anaerobic pathogen Bacteroides fragilis has an essential requirement for both heme and free iron to support growth in extraintestinal infections. In the absence of free iron, B.
Edson R. Rocha, Hector A. Bergonia, Svetlana Gerdes, Charles Jeffrey Smith   +3 more
doaj   +1 more source