Results 71 to 80 of about 23,652 (216)

Prefolding and quality control of OMPs in periplasmic space (224.1)

The FASEB Journal, 2014
The outer membrane proteins (OMPs) of Gram‐negative bacteria have to be translocated through the periplasmic space before reaching their final destination. The aqueous environment of the periplasmic space and high permeability of the outer membrane engender such a translocation process inevitably challenging. In
openaire   +1 more source

A Hanks‐type bacterial kinase, PknS, directly phosphorylates the alternative sigma factor EcfK to promote resistance to protist predation

The FEBS Journal, EarlyView.
The Xanthomonas citri Hanks‐type kinase PknS autophosphorylates and directly phosphorylates the alternative sigma factor EcfK at five residues. Besides the conserved residue T51 in the σ2 domain, phosphorylation of a residue in the linker between σ2 and σ4 is critical for EcfK activation by promoting its interaction with a positively charged pocket in ...
Lídia dos Passos Lima, Dev Sriranganadane, Daiane Laise da Silva, Natália C. Drebes Dörr, Enzo Breviglieri Sichi Mello, Caio Vinicius dos Reis, Rogério Ferreira Lourenço, José Felipe Teixeira da Silva Santos, Anita Salmazo, Brenno Wendler Miranda, Katlin B. Massirer, Rafael M. Couñago, Cristina E. Alvarez‐Martinez   +12 more
wiley   +1 more source

Conserved substrate binding by chaperones in the bacterial periplasm and the mitochondrial intermembrane space

Biochemical Journal, 2007
Mitochondria were derived from intracellular bacteria and the mitochondrial intermembrane space is topologically equivalent to the bacterial periplasm. Both compartments contain ATP-independent chaperones involved in the transport of hydrophobic membrane proteins.
Alcock, Felicity H., Grossmann, J. Gunter., Gentle, Ian Edward., Likic, Vladimir A., Lithgow, Trevor., Tokatlidis, Kostas.   +5 more
openaire   +3 more sources

The Keystone‐Pathogen Hypothesis Updated: The Role of Porphyromonas gingivalis in Periodontitis

Journal of Periodontal Research, EarlyView.
Porphyromonas gingivalis orchestrates a coordinated manipulation of immune and inflammatory responses in periodontal tissues which leads to the generation of a dysbiotic, subgingival biofilm community, and progression of periodontitis. The type 9 secretion system, lipid A modification, and the formation of outer membrane vesicles are important ...
Mike A. Curtis, James A. Garnett, Richard P. Darveau   +2 more
wiley   +1 more source

Optimization of Tris/EDTA/Sucrose (TES) periplasmic extraction for the recovery of functional scFv antibodies

AMB Express, 2020
Single-chain variable fragments (scFvs) have gained increased attention among researchers in both academic and industrial fields owing to simple production in E. coli. The E. coli periplasm has been the site of choice for the expression of scFv molecules
Elham Ghamghami, Marjan Abri Aghdam, Mohammad Reza Tohidkia, Asadollah Ahmadikhah, Morteza Khanmohammadi, Tayebeh Mehdipour, Ahad Mokhtarzadeh, Behzad Baradaran   +7 more
doaj   +1 more source

Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose Epimerase

Molecular Microbiology, EarlyView.
Model for the non‐PTS utilization of manno‐oligosaccharides by Cellvibrio japonicus. The Mgp130A and EpiA enzymes are essential for the utilization of mannose residues. ABSTRACT Manno‐oligosaccharides and their metabolism play important roles in gut health, pharmaceutical development, and renewable chemical production.
Jessica K. Novak, Jeffrey G. Gardner
wiley   +1 more source

A novel single‐cell NAD‐ME C4 subtype integrated with CAM and bicarbonate use in an aquatic plant

New Phytologist, EarlyView.
Summary Many plants maximize photosynthesis by using a CO2‐concentrating mechanism (CCM). Based on physiology, the freshwater plant Ottelia alismoides has three CCMs: C4 metabolism (NAD‐malic enzyme (NAD‐ME) subtype) and bicarbonate‐use during the day plus crassulacean acid metabolism (CAM) at night and lacks Kranz anatomy. Here, we combined a range of
Hong Sheng Jiang, Wenmin Huang, Shijuan Han, Pengpeng Li, Zuying Liao, Liyuan Wei, Lei Zhao, Shuping Gu, Jun Ding, Brigitte Gontero, Stephen C. Maberly, Wei Li   +11 more
wiley   +1 more source

Cobalt(II) bioaccumulation and distribution in Rhodopseudomonas palustris

Biotechnology & Biotechnological Equipment, 2017
Bioaccumulation by growing cells is a potential technique for the removal of heavy metals from wastewater. Cobalt bioaccumulation by Rhodopseudomonas palustris via different metabolic pathways was investigated for various pH levels, temperatures, co ...
Rui Gao, Yiming Wang, Yefei Zhang, Jin Tong, Wei Dai   +4 more
doaj   +1 more source

Cloning, expression and purification of recombinant streptokinase: partial characterization of the protein expressed in Escherichia coli

Brazilian Journal of Medical and Biological Research, 1997
We cloned the streptokinase (STK) gene of Streptococcus equisimilis in an expression vector of Escherichia coli to overexpress the profibrinolytic protein under the control of a tac promoter. Almost all the recombinant STK was exported to the periplasmic
L. Avilán, A. Yarzábal, C. Jürgensen, M. Bastidas, J. Cruz, J. Puig   +5 more
doaj   +1 more source

High‐Efficiency and Eco‐Friendly Management of Multiple Fungal Diseases by a Novel Carbon Dots sCDP Delivered RNA Nano‐Pesticides

Plant Biotechnology Journal, EarlyView.
ABSTRACT RNA‐based pesticides represent a transformative approach in agricultural disease management, offering an environmentally sustainable alternative to conventional chemical fungicides. However, key challenges such as rapid RNA degradation in the environment and inefficient uptake hinder widespread adoption.
Chunhao Jiang, Jijie Cui, Yumeng Wang, Ziyi Zhang, Yi Yang, Tao Tang, Junjie Wang, Daijing Li, Zihan Zhao, Kuaibing Wang, Dongdong Niu   +10 more
wiley   +1 more source